球孢白僵菌对象虫金小蜂安全性评价和亚致死效应研究

为明确球孢白僵菌Beauveria bassiana对烟草甲Lasioderma serricorne(Fabricius)幼虫、成虫的致病力以及对象虫金小蜂Anisopteromalus calandrae(Howard)的安全性,本研究采用浸渍法测定球孢白僵菌对烟草甲和象虫金小蜂的LC₁₀、LC₂₅、LC₅₀,以球孢白僵菌亚致死浓度LC₁₀和LC₂₅胁迫象虫金小蜂成虫后,分析其F₁代实验种群生存、发育和繁殖的变化。结果表明：(1)在1×10⁹孢子/mL的球孢白僵菌浓度下,烟草甲幼虫的致死率可达到92.62%,烟草甲成虫的致死率为100.00%,象虫金小蜂成虫的致死率可达98.73%,亚致死浓度LC₁₀和LC₂₅分别为2.125×10⁵和7.114×10⁵孢子/mL。(2)受LC₁₀和LC₂₅胁迫处理象...     

On the circumscription of Paeonia kesrouanensis, an east Mediterranean peony

Two populations of Paeonia turcica, including one in the type locality, and two populations of P. kesrouanensis were sampled and observed, in addition to examination of a large number of herbarium specimens. As a result of extensive comparison, we found that there are no distinct differences between P. turcica and P. kesrouanensis, and thus P. turcica is treated as a synonym of the latter. The relationships of P. kesrouanensis with its allies are discussed.     

小型猪动脉粥样硬化斑块稳定性模型研究

目前已有的动物模型在研究动脉粥样硬化斑块破裂、破裂的可控性及量化研究方面均不能满足研究的需要.为了建立类似于人类动脉粥样硬化病变的斑块模型,体外研究斑块稳定性,应用传统的高脂高胆固醇膳食诱导建立了小型猪动脉粥样硬化模型,并从血脂水平和斑块病理形态学特征方面加以了证实.该模型中斑块与人类成熟斑块的高度相似性使其成为研究斑块稳定性和斑块破裂的较好模型.从量化比较这一出发点着手,建立了一个体外可控可量化诱导斑块破裂模型,方法简单易行,是一个较好的量化研究斑块破裂和破裂相关因素间关系的实验模型.     

合成生物学发展现状与前景

合成生物学是综合了科学与工程的一个崭新的生物学研究领域,为生命现象及其运动规律的解析提供了一种采用“白下而上”合成策略的正向工程学的研究思路和方法手段,在经济和社会发展中具有巨大的应用开发潜力。近年来,DNA合成与系统生物学技术的发展使生命系统复杂基因回路的设计、合成与组装逐步成为可能,并应用于生物基化学品、生物燃料、医药中间体、保健产品的生产和环境保护等领域。但是,合成生物学的研究仍然面临科学、技术和伦理的挑战,只有积极地应对这些问题,在加大研究开发支持力度的同时,做好必要的风险监管,才能真正把握合成生物学发展带来的历史机遇。     

Identification and Assessing the Cultivars of Laminaria Lamx. （Phaeophyceae） with Molecular Markers

Molecular markers were used to identify and assess cultivars ofLaminaria Lamx. and to delineate their phylogenetic relationships. Random amplified polymorphic DNA (RAPD) analysis was used for detection. After screening, 11 primers were selected and they yielded 133 bands in all, of which approximately 99.2% were polymorphic. The genetic distances between gametophytes ranged from 0.412 to 0.956.Two clusters were formed with the unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the simple matching coefficient. All cultivars ofLaminariajaponica Aresch. used for breeding in China fell into one cluster. L.japonica from Japan, L. saccharina (L.) Lam., and L. angustata Kjellm.formed the other cluster and showed higher genetic variation than L. japonica from China. Nuclear ribosomal DNA (rDNA) sequences, including internal transcribed spacers (ITS1 and ITS2) were studied and aligned. The nucleotides of the sequences ranged from 634 to 668, with a total of 692 positions including ITS1, ITS2, and the 5.8S coding region. The phylogenetic tree obtained by the neighbor-joining method favored, to some extent, the results revealed by RAPD analysis. The present study indicates that RAPD and ITS analyses could be used to identify and assess Laminaria germplasm and to distinguish some species and, even intraspecies, in Laminaria.     

来源嗜碱性芽孢杆菌N-227β-环糊精葡基转移酶基因分析及在大肠杆菌中的诱导表达

很多细菌可以产生环糊精葡基转移酶,对来源于嗜碱性芽孢杆菌N-227菌株染色体上一段编码β-环糊精葡基转移酶基因、包含有自己的启动子且能够直接在大肠杆菌中表达的DNA序列进行测序分析,该片断DNA包含有4114bp,从745位点到2883位点包含2139个碱基,为一个推定的编码713个氨基酸的蛋白质阅读框,和来源于Bacillus circulansA11的β-环糊精葡基转移酶氨基酸完全一致;具有环糊精葡基转移酶典型的五个结构域A-E,在A-B结构域中包含有七个属于α-淀粉酶家族的保守区域(I-VII)。对该酶基因进行PCR并克隆到表达载体pET28b上,利用乳糖进行诱导表达,获得了高效表达,环化活性为11.75mg/min/mL。这对于β-环糊精葡基转移酶的应用和降低成本具有重要的价值。     

Plasmon-Induced Transparency in a Surface Plasmon Polariton Waveguide with a Right-Angled Slot and Rectangle Cavity

Plasmonics - The phenomenon of plasmon-induced transparency (PIT) is realized a in surface plasmon polariton waveguide at near-infrared frequencies. The right-angled slot and rectangle cavity...     

γ-Tocotrienol Induces Paraptosis-Like Cell Death in Human Colon Carcinoma SW620 Cells

Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.     

百山祖常绿阔叶林优势种群枯死木的结构特征

为了了解常绿阔叶林优势种群的枯死树木结构特征,对百山祖5 hm2固定样地的10个优势种群10年间的枯死木和现有活立木进行了统计分析。结果表明:(1)枯死木龄级呈"倒J型"分布的种群有厚叶红淡比、水丝梨、凸脉冬青、褐叶青冈、短尾柯、多脉青冈、亮叶水青冈、木荷等8种,而麂角杜鹃和石灰花楸则呈中峰型曲线分布(高峰出现在Ⅱ龄级)。枯死木主要集中在Ⅰ~Ⅲ龄级。(2)群落各优势种群的枯死比例在Ⅰ龄级最大,短尾柯和麂角杜鹃在Ⅰ龄级的枯死比例分别高达50.00%和47.61%。随着龄级的增大,枯死比例下降,在Ⅳ龄级以后各优势种群的枯死比例发生变化。平均枯死比例最大的是石灰花楸(30.42%),最小的是亮叶水青冈(13.70%)。(3)在种群活立木密度较大的生境,其枯死木密度也较大。水丝梨在沟谷的枯死木密度较大,多脉青冈在4种生境中的枯死木密度差别不大,其余8个种群都表现出枯死木密度在山脊大、沟谷小的特点。石灰花楸在陡坡(42.86%)、凸脉冬青在沟谷(29.78%)的枯死比例较大,亮叶水青冈在山脊(12.40%)的枯死比例最小。