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31.
Therapeutic concentrations of varenicline in the presence of nicotine increase action potential firing in human adrenal chromaffin cells
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Evidence for sexual dimorphism is extremely limited in the non‐avian dinosaurs despite their high diversity and disparity, and despite the fact that dimorphism is very common in vertebrate lineages of all kinds. Using body‐size data from both Alligator mississippiensis and Rhea americana, which phylogenetically bracket the dinosaurs, we demonstrate that even when there is strong dimorphism in a species, random sampling of populations of individuals characterized by sustained periods of growth (as in the alligator and most dinosaurs) can result in the loss of this signal. Dimorphism may be common in fossil taxa but very hard to detect without ontogenetic age control and large sample sizes, both of which are hampered by the limitations of the fossil record. Signal detection may be further hindered by Type III survivorship, whereby increased mortality among the young favours the likelihood that they will be sampled (unless predation or taphonomic bias against small size acts against this). These, and other considerations relating to behaviour and ecology, provide powerful reasons to suggest that sexual dimorphism in dinosaurs may be very difficult to detect in almost all currently available samples. Similar issues are likely also to be applicable to many fossil reptiles, or animals more generally. 相似文献
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Vaccination with a Shigella DNA vaccine vector induces antigen-specific CD8(+) T cells and antiviral protective immunity
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A prototype Shigella human immunodeficiency virus type 1 (HIV-1) gp120 DNA vaccine vector was constructed and evaluated for immunogenicity in a murine model. For comparative purposes, mice were also vaccinated with a vaccinia virus-env (vaccinia-env) vector or the gp120 DNA vaccine alone. Enumeration of the CD8(+)-T-cell responses to gp120 after vaccination using a gamma interferon enzyme-linked spot assay revealed that a single intranasal dose of the Shigella HIV-1 gp120 DNA vaccine vector elicited a CD8(+) T-cell response to gp120, the magnitude of which was comparable to the sizes of the analogous responses to gp120 that developed in mice vaccinated intraperitoneally with the vaccinia-env vector or intramuscularly with the gp120 DNA vaccine. In addition, a single dose of the Shigella gp120 DNA vaccine vector afforded significant protection against a vaccinia-env challenge. Moreover, the number of vaccinia-env PFU recovered in mice vaccinated intranasally with the Shigella vector was about fivefold less than the number recovered from mice vaccinated intramuscularly with the gp120 DNA vaccine. Since the Shigella vector did not express detectable levels of gp120, this report confirms that Shigella vectors are capable of delivering passenger DNA vaccines to host cells and inducing robust CD8(+) T-cell responses to antigens expressed by the DNA vaccines. Furthermore, to our knowledge, this is the first documentation of antiviral protective immunity following vaccination with a live Shigella DNA vaccine vector. 相似文献
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Cope's Rule is the tendency for organisms in evolving lineages to increase in size over time. The concept is detailed in many textbooks, but has rarely been demonstrated. Many suggestions of the benefits of large body size exist, but none has yet been confirmed empirically. Using a large-scale analysis of recent studies, Kingsolver and Pfennig have now shown how size benefits survival, mating success and fecundity, and they provide convincing arguments for a mechanism that is capable of driving Cope's Rule. 相似文献
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D. Clarke I. Gorman F. Ringholz M. McDermott DW. Cox P. Greally B. Linnane P. Mc Nally 《Respiratory research》2018,19(1):255
Pulmonary aspiration of gastric refluxate (PAGR) has been demonstrated in association with pulmonary inflammation in school aged children with Cystic Fibrosis (CF). We sought to determine if similar findings were present in preschool children. Pepsin was measured in Broncho-alveolar lavage (BAL) fluid collected from clinically stable preschool children with CF and controls. Elevated pepsin levels were found in a subgroup of children with CF, but this was not found to be associated with pulmonary infection, pulmonary inflammation or respiratory or gastrointestinal symptoms. 相似文献
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Nils C. Gauthier Olivier M. Rossier Anurag Mathur James C. Hone Michael P. Sheetz 《Molecular biology of the cell》2009,20(14):3261-3272
The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and dynamic assays to follow exocytosis, endocytosis, and PM area changes during fibroblast spreading. Because the PM area cannot increase by stretch, spreading proceeds by the flattening of membrane folds and/or by the addition of new membrane. Using laser tweezers, we found that PM tension progressively decreases during spreading, suggesting the addition of new membrane. Next, we found that exocytosis increases the PM area by 40–60% during spreading. Reducing PM area reduced spread area, and, in a reciprocal manner, reducing spreadable area reduced PM area, indicating the interconnection between these two parameters. We observed that Golgi, lysosomes, and glycosylphosphatidylinositol-anchored protein vesicles are exocytosed during spreading, but endoplasmic reticulum and transferrin receptor-containing vesicles are not. Microtubule depolymerization blocks lysosome and Golgi exocytosis but not the exocytosis of glycosylphosphatidylinositol-anchored protein vesicles or PM area increase. Therefore, we suggest that fibroblasts are able to regulate about half of their original PM area by the addition of membrane via a glycosylphosphatidylinositol-anchored protein compartment. 相似文献
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Nils Gauthier Nicolas Biais Wynn Vonnegut Marc‐Antoine Fardin Philip Avigan Evan R Heller Anurag Mathur Saba Ghassemi Michael S Koeckert James C Hone Michael P Sheetz 《The EMBO journal》2010,29(6):1055-1068
Extracellular matrices in vivo are heterogeneous structures containing gaps that cells bridge with an actomyosin network. To understand the basis of bridging, we plated cells on surfaces patterned with fibronectin (FN)‐coated stripes separated by non‐adhesive regions. Bridges developed large tensions where concave cell edges were anchored to FN by adhesion sites. Actomyosin complexes assembled near those sites (both actin and myosin filaments) and moved towards the centre of the non‐adhesive regions in a treadmilling network. Inhibition of myosin‐II (MII) or Rho‐kinase collapsed bridges, whereas extension continued over adhesive areas. Inhibition of actin polymerization (latrunculin‐A, jasplakinolide) also collapsed the actomyosin network. We suggest that MII has distinct functions at different bridge regions: (1) at the concave edges of bridges, MIIA force stimulates actin filament assembly at adhesions and (2) in the body of bridges, myosin cross‐links actin filaments and stimulates actomyosin network healing when breaks occur. Both activities ensure turnover of actin networks needed to maintain stable bridges from one adhesive region to another. 相似文献
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David W. E. Hone John M. Ratcliffe Daniel K. Riskin John W. Hermanson Robert R. Reisz 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2021,54(1):106-112
Rhamphorhynchus muensteri is one of the best‐known flying reptiles, represented by >130 well‐preserved fossil specimens, from hatchlings to full adults. The life history of this pterosaur remains controversial as to when in ontogeny they took flight. Here, we assess the growth of these animals based on the lengths of numerous key elements. We show that changes in the skeletal anatomy of this reptile across its post‐hatch size range reveal that R.muensteri exhibited overall near isometric growth in the wings, with slightly negative allometry in the humerus, radius and stronger negative allometry in the fourth metacarpal compared to body length, and slightly positive allometry in the second and third phalanges compared to body length. This pattern is near unique among flying vertebrates and suggests R.muensteri flew soon after hatching. In bats and birds, offspring do not typically fly until nearly adult sized. Conversely, near isometric growth in Rhamphorhynchus suggests it was a precocial flier and that individuals may have inhabited several sequential foraging niches over their lifespan, as some terrestrial and aquatic vertebrates do today. 相似文献