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21.
M.H. TVEY T.A. DOWNS M.C. HART D.C. GRAVES 《The Journal of eukaryotic microbiology》1989,36(1):38S-41S
The sequential appearance of Pneumocystis carinii (Pc) antigens during the progression of immunosuppression in rats was studied using the immunobloiting technique and specific immunologic probes. Putative Pc soluble antigens, with molecular weights of approximately 70 and 90 kd. were delected as early as 2 wk after initiation of immunosuppression in rats using a pool of monoclonal antibodies produced to I c isolated from lungs using enzymatic digestion. Monoclonal antibodies produced to Pc isolated by massaging the lung tissue using a Stomacher apparatus and infection-derived sera did not detect soluble antigens until at least the 6th wk of immunosuppression. Analysis of Pc pellets obtained from Stomacher- and lavage-processcd lungs revealed that the lower molecular weight antigens (≤ 40, 45 and possibly 55–60 kd) were recognized early during the immunosuppression process. 相似文献
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In a paper by Zimmermann and colleagues in this issue of Arthritis Research & Therapy, results of extended laboratory research with the drug combination of prednisolone and dipyridamole are reported. There seems
to be a boost and extension of the glucocorticoid effect by the combination, without a clear increase of adverse effects,
potentially allowing the application of lower dosages. However, laboratory models are not patients and the glucocorticoid
mechanisms leading to effects and adverse effects are manifold. The next required step will be to demonstrate the improved
therapeutic window in patients in adequate comparative clinical trials, assessing predefined beneficial effects and adverse
effects in a standardized way. 相似文献
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Kumaresan Kavitha Subramanian Mathiyazhagan Vaithiyanathan Sendhilvel Sevagaperumal Nakkeeran GOPAL Chandrasekar WG Dilantha Fernando 《Archives Of Phytopathology And Plant Protection》2013,46(1):69-76
Antifungal antibiotic from Pseudomonas chlororaphis isolate PA23 was identified as Phenazine using TLC and HPLC. Phenazine recorded the highest inhibition zone of 21?mm with 35.55% percent inhibition of mycelial growth of Pythium aphanidermatum over control. It had a significant effect on the hyphal morphology of P. aphanidermatum and on spore germination of Botryodiplodia theobromae and Alternaria solani. Disorganization of hyphal morphology of P. aphanidermatum includes vacuolization, cell content degeneration and hyphal lysis. Similarly interaction of phenazine with Rhizoctonia solani resulted in abnormal swelling of hyphal tips was noticed in the hyphal tips. Similarly the germination of sclerotia of Macrophomina phaseolina, R. solani and Sclerotium rolfsii were completely inhibited by phenazine at a concentration 50?μl. Incubation of the eggs of the root knot nematode Meloidogyne incognita in 30?μl concentration of phenazine, completely suppressed the hatching of juveniles. 相似文献
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