全文获取类型
收费全文 | 789篇 |
免费 | 87篇 |
专业分类
876篇 |
出版年
2023年 | 8篇 |
2022年 | 9篇 |
2021年 | 16篇 |
2019年 | 13篇 |
2018年 | 14篇 |
2017年 | 10篇 |
2016年 | 11篇 |
2015年 | 29篇 |
2014年 | 21篇 |
2013年 | 39篇 |
2012年 | 52篇 |
2011年 | 50篇 |
2010年 | 27篇 |
2009年 | 27篇 |
2008年 | 41篇 |
2007年 | 43篇 |
2006年 | 40篇 |
2005年 | 21篇 |
2004年 | 20篇 |
2003年 | 30篇 |
2002年 | 14篇 |
2001年 | 21篇 |
2000年 | 19篇 |
1999年 | 10篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1993年 | 6篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 17篇 |
1989年 | 7篇 |
1988年 | 13篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 8篇 |
1980年 | 6篇 |
1979年 | 13篇 |
1978年 | 6篇 |
1977年 | 10篇 |
1976年 | 12篇 |
1975年 | 15篇 |
1974年 | 19篇 |
1973年 | 13篇 |
1972年 | 9篇 |
1971年 | 7篇 |
1970年 | 10篇 |
1969年 | 5篇 |
1965年 | 6篇 |
排序方式: 共有876条查询结果,搜索用时 0 毫秒
41.
Koffler Christoph Amor Ben Carbajales-Dale Michael Cascio Joseph Conroy Alison Fava James A. Gaudreault Caroline Gloria Thomas Hensler Connie Horvath Arpad Humbert Sebastien Manzardo Alessandro Margni Manuele Osset Philippe Sinistore Julie Vigon Bruce Wallace Michele L Wang Michael Prox Martina 《The International Journal of Life Cycle Assessment》2020,25(3):478-482
The International Journal of Life Cycle Assessment - 相似文献
42.
Jeremy J. Ramsden Robert Horvath 《Journal of receptor and signal transduction research》2013,33(3-4):211-223
Planar optical waveguides offer an ideal substratum for cells on which to reside. The materials from which the waveguides are made—high refractive index transparent dielectrics—correspond to the coatings of medical implants (e.g., the oxides of niobium, tantalum, and titanium) or the high molecular weight polymers used for culture flasks (e.g., polystyrene). The waveguides can furthermore be modified both chemically and morphologically while retaining their full capability for generating an evanescent optical field that has its greatest strength at the interface between the solid substratum and the liquid phase with which it is invariably in contact (i.e., the culture medium bathing the cells), decaying exponentially perpendicular to the interface at a rate controllable by varying the material parameters of the waveguide. Analysis of the perturbation of the evanescent field by the presence of living cells within it enables their size, number density, shape, refractive index (linked to their constitution) and so forth to be determined, the number of parameters depending on the number of waveguide lightmodes analyzed. No labeling of any kind is necessary, and convenient measurement setups are fully compatible with maintaining the cells in their usual environment. If the temporal evolution of the perturbation is analyzed, even more information can be obtained, such as the amount of material (microexudate) secreted by the cell while residing on the surface. Separation of parallel effects simultaneously contributing to the perturbation of the evanescent field can be accomplished by analysis of coupling peak shape when a grating coupler is used to measure the propagation constants of the waveguide lightmodes. 相似文献
43.
Z. Horvath P. Saiko C. Illmer S. Madlener T. Hoechtl W. Bauer 《Nucleosides, nucleotides & nucleic acids》2013,32(9-11):1019-1024
Resveratrol (RV), a naturally occurring stilbene derivative, is a potent free radical scavenger causing a number of biochemical and antineoplastic effects. It was shown to induce differentiation and apoptosis in leukemia cells and was also identified as an inhibitor of ribonucleotide reductase (RR), a key enzyme of DNA synthesis. In this study, we report about the biochemical effects of RV in HL-60 human promyelocytic leukemia cells. RV effectively inhibited in situ RR activity. Furthermore, incubation of HL-60 cells with RV significantly decreased intracellular dCTP, dTTP, dATP and dGTP concentrations. In growth inhibition and clonogenic assays, RV acted synergistically with both Ara-C and tiazofurin in HL-60 cells. We conclude that RV could become a viable candidate as one compound in the combination chemotherapy of leukemia and therefore deserves further in vitro and in vivo testing. 相似文献
44.
Vivienne C.M. Neeve Angela Pyle Veronika Boczonadi Aurora Gomez-Duran Helen Griffin Mauro Santibanez-Koref Ulrike Gaiser Peter Bauer Andreas Tzschach Patrick F. Chinnery Rita Horvath 《Mitochondrion》2013,13(6):743-748
Exome sequencing identified compound heterozygous mutations in the recently discovered mitochondrial methionyl-tRNA formyltransferase (MTFMT) gene in two sisters with mild Leigh syndrome and combined respiratory chain deficiency. The mutations lead to undetectable levels of the MTFMT protein. Blue native polyacrylamide gel electrophoresis showed decreased complexes I and IV, and additional products stained with complex V antibodies, however the overall steady state level of mt-tRNAMet was normal. Our data illustrate that exome sequencing is an excellent diagnostic tool, and its value in clinical medicine is enormous, however it can only be optimally exploited if combined with detailed phenotyping and functional studies. 相似文献
45.
46.
Daniela Niemeyer Thomas Zillinger Doreen Muth Florian Zielecki Gabor Horvath Tasnim Suliman Winfried Barchet Friedemann Weber Christian Drosten Marcel A. Müller 《Journal of virology》2013,87(22):12489-12495
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory infection with as yet unclear epidemiology. We previously showed that MERS-CoV counteracts parts of the innate immune response in human bronchiolar cells. Here we analyzed accessory proteins 3, 4a, 4b, and 5 for their abilities to inhibit the type I interferon response. Accessory protein 4a was found to block interferon induction at the level of melanoma differentiation-associated protein 5 (MDA5) activation presumably by direct interaction with double-stranded RNA. 相似文献
47.
A Rita Horvath 《The Clinical biochemist. Reviews / Australian Association of Clinical Biochemists》2013,34(2):47-60
Laboratory tests offer value if they provide benefit to patients at acceptable costs. Laboratory testing is one of the most widely used diagnostic interventions supporting medical decisions, yet evidence demonstrating its value and impact on health outcomes is limited. This contributes to wide variations in test utilisation including underdiagnosis, overdiagnosis and misdiagnosis, which may impact the quality and the clinical- and cost-effectiveness of care and patient safety. Therefore implementing evidence into the care of patients is a moral and social imperative to laboratory professionals and all health care staff.This review investigates the reasons research does not get into practice, or only does with a very long delay. Apart from reviewing the common barriers to implementation, it also discusses the drivers of inappropriate test utilisation. By reviewing the theoretical and practical aspects of implementation science, recommendations are made for approaches that are thought to be most effective and that can be adopted to close the gap between evidence and practice, and to facilitate evidence-based laboratory medicine. Passive dissemination of the evidence and educational interventions are insufficient and do not offer sustainable solutions. A multifaceted and individualised implementation strategy, including individually tailored academic detailing, reminder systems, clinical decision support systems, feedback on performance, and participation of doctors and laboratory professionals in quality improvement activities addressing test selection and interpretation and in clinical audits, has greater potential for success. Examples of these initiatives at the laboratory and clinical interface are provided with links to valuable resources.
‘Knowing is not enough; we must apply.Willing is not enough; we must do.’JW von Goethe相似文献
48.
49.
50.