中国姬蜂科(膜翅目)二新种(英文)

本文报道采自河南省伏牛山区的姬蜂科Ichneumonidae二新种 :暗足大食姬蜂ZabrachypusatripedalisSheng ,sp .nov .和斑三钩姬蜂TriancyramaculataSheng ,sp .nov .各新种分别与其近似种作了比较。标本保存在国家林业局森林病虫害防治总站     

镰尾姬蜂属一新种(膜翅目:姬蜂科)(英文)

本文记述镰尾姬蜂属Grypocentrus Ruthe一新种:卡镰尾姬蜂Grypocentrus kasparyani sp.nov.本种与室镰尾姬蜂G.areolaris Kasparyan相似,以下特征可与后者区别:唇基宽为长的2.7-3.0倍:眼颚距为上颚基部宽的0.10-0.15倍;触角鞭节16-18节(绝大多数为17节);卵柄较长也较细。正模,1991-06-09;配模,1993-05-30;副模:1,1991-06-09,2,2,1993-05-30;辽宁沈阳,盛茂领采。     

Agrobacterium tumefaciens-mediated genetic transformation of haptophytes (Isochrysis species)

Isochrysis galbana and Isochrysis sp. are economically important microalgae from the division of haptophytes. Here, we report Agrobacterium-mediated stable DNA transfer into their nuclear genomes. Initial studies were performed to standardize co-cultivation media and determine the sensitivity of the microalgae to selective agents. Up to 1 mg/ml of the antibiotic hygromycin did not inhibit growth, whereas both the haptophytes bleached in artificial seawater (ASW) medium containing micromolar concentrations of the herbicide norflurazon. Co-cultivation of Isochrysis sp. and I. galbana with Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector pCAMBIA 1380-pds-L504R yielded norflurazon-resistant (NR) colonies visible on selective plates after 20–30 days. pCAMBIA 1380-pds-L540R was constructed by cloning a mutated genomic phytoene desaturase (pds) gene from Haematococcus pluvialis as a selectable marker gene into the binary vector system pCAMBIA 1380. Co-cultivation of Isochrysis sp. with A. tumefaciens in ASW medium containing 200 μM of acetosyringone for 72 h produced the highest number of NR cells. For I. galbana, 100 μM of acetosyringone, ASW medium, and 48 h co-cultivation period appeared to be optimum co-cultivation parameters. The NR colonies kept their resistance phenotype for at least 24 months, even in the absence of selective pressure. The transfer of the pds gene in NR cells was shown by PCR amplification of the T-DNA sequences from the genomic DNA of NR cells and Southern blot analysis using T-DNA sequences as probes. The genetic manipulation described here will allow metabolic engineering and a better understanding of several biochemical pathways in the future.