Excirolana braziliensis, a Pan-American sand beach isopod: taxonomic status, zonation and distribution

Excirolana braziliensis Richardson is shown to be the senior synonym of Cirolana salvadorensis Schuster and C. koepckei Bott. The latter two species, incorrectly assigned to Cirolana , were formerly regarded as distinct and were separated on the basis of size-dependent characters. Sampling of sandy beaches along the eastern Pacific from Mexico to Chile (21 localities) and the western Atlantic from Mexico to Brazil, including the West Indies (20 localities), demonstrates that E. braziliensis is commonly the most abundant macroscopic organism present in this habitat. Marked differences in zonation of young and adults are illustrated from data in Panama.     

A comparative morphometric study on the ultrastructure of adherent cells in long-term bone marrow culture from normal and congenitally anemic mice

The relationship between structure and function of bone marrow stromal tissue in adherent layers of long-term bone marrow cultures (LTBMCs) from normal and congenital anemic mice (C57BL, Sl/Sld, Sl+/Sl+, W/Wv, and W+/W+) was investigated. Many previously reported features were confirmed. However, in LTBMC from all strains of mice examined, isolated cilia with the axonemal structure of a 9 + O pattern with obvious dynein arms were observed in the blanket cells. The frequency of cilia was approximately 2%-5% of total number of profiles of blanket cells examined. Crystalloid inclusions (CI) were observed in cultured macrophages similar to those reported in vivo in all strains of murine LTBMC. The CI could be classified into four types according to their structure in the same way as in vivo (type A to type D), with a predominance of type A in the cultures. Viral particles were also apparent in adherent cells of all strains (except W/Wv and W+/W+), which were compatible with a type C retrovirus. Gap junctions occurred regularly between the adherent cells of LTBMC, particularly between blanket cells and preadipocytes. The most frequent appearance of gap junctions was found in Sl/Sld cultures. The phenomena of normal and abnormal hematopoiesis appear to be accurately reproduced in culture, thus retaining the same relationship between function and structure as occurs in vivo. The surface of isolated cilia of blanket cells, CI of macrophages, viral particles among adherent cells, and gap junctions between blanket cells and preadipocytes is discussed.     

Can evolutionary theories of dispersal and senescence predict postrelease survival,dispersal, and body condition of a reintroduced threatened mammal?

1. Theories of dispersal and senescence (or aging) predict that dispersal, and ongoing survival and body condition, are influenced by evolutionary drivers, along with intrinsic and extrinsic factors. Such theories are relevant to translocations of animals where high mortality, loss of body condition, and dispersal beyond the area of release are commonly reported. However, these theories have rarely been tested using data from translocations.
2. We explore whether theories of dispersal and senescence, together with biological knowledge and management interventions, can predict rates of postrelease dispersal, survival and change in body condition of a translocated endangered meso‐predator, the eastern quoll Dasyurus viverrinus.
3. Captive‐bred quolls (n = 60) from three sanctuaries were translocated to an unfenced, predator‐managed reserve (Booderee National Park) over 2 years (2018, 2019). Survival, dispersal and body mass were monitored via GPS/VHF tracking and targeted trapping for 45 days postrelease.
4. We found support for the “social subordinate” hypothesis, with smaller quolls dispersing further. Consistent with theories of senescence and the biology of our species, survival was marginally greater for females, and females regained losses in body mass in both years following release. In contrast, males recovered body condition in the first but not the second release as this coincided with breeding. Quolls that originated from the mainland sanctuary were on average heavier at release and, after accounting for weight, dispersed further.
5. Synthesis and applications. Using theory to test outcomes of wildlife translocations can provide insights into patterns across taxa and under different conditions, enabling useful improvements to future fauna translocations. This allows for better predictions to be made about the likelihood of success from proposed translocations, changes to planning to improve outcomes (e.g., modifying sex ratios, individual selection and release cohort), and improved animal welfare as fewer animals are subjected to trials.

     

Circular dichroism of nucleic acid monomers. II. Derivation and application to polymers of a consistent set of guanine and cytosine transition parameters

An approximate semiempirical procedure has been developed in order to derive nucleic acid monomer π → π* electronic transition moment parameters. Using the approximate procedure, guanine (G) and cytosine (C) transition moment parameters have been derived from agreement found between calculated weight-averaged and measured CD spectra of cyclic-GMP and cyclic-CMP. The derived base transition moment parameters have been assessed in CD spectral calculations on some G- and C-containing nucleic acids for which reasonably good structural information exists. An attempt was also made at evaluating the likely CD spectral contributions of G and C electric n → π* transition moments whose magnitudes were taken to be the maximum expected. Overall, the results indicate that the derived G and C π → π* transition moment parameters are more successful in nucleic acid CD spectral calculations than those used in previous DeVoe theory CD calculations. In addition, the results indicate that electric n → π* transitions may be of importance in understanding nucleic acid monomer CD spectra but appear to be relatively unimportant in understanding nucleic acid polymer CD spectra. It is concluded that the derived G and C π → π* parameters are more useful in DeVoe theory CD calculations than parameters used previously.     

Historical aspects of F. W. putnam's systematic studies on fishes

Summary As a student and collaborator of Louis Agassiz on the study of fishes, F. W. Putnam gave promise of becoming a leading ichthyologist with special interest in taxonomy generally and the Etheostomidae in particular. While he was noted briefly in these fields, contributed a number of minor papers, and aided in the posthumous publications of some of Agassiz's work on fishes, he neither reached his original goal nor completed his major projected works. For in 1874 he switched careers and was appointed Curator of the Peabody Museum of American Archaeology and Ethnology at Harvard University, and is remembered today primarily as a founder of American archaeology rather than as a systematic ichthyologist.Paper read at the 19th annual meeting of the Society of Systematic Zoology, New York City, 27 December 1967. Quotations are taken from the F. W. Putnam papers in the Archives of Harvard University, with permission of the Archives and the Putnam family.     

Marrow grown in long-term culture can be used for autologous transplantation in the treatment of acute myeloid leukaemia

Leukaemic cells can be selectively depleted in the conditions of long-term bone marrow culture (LTBMC). Use can be made of LTBMC to purge bone marrow of leukaemic cells in autologous bone marrow transplantation (ABMT).     

Desalting of nucleotides by reverse-phase high-performance liquid chromatography

Chromatography of AMP, NAD⁺, or NADH on a reverse-phase C₁₈ Porasil B column rapidly removes ammonium formate or potassium phosphate from 90% of the nucleotide. Earlier reports showed these salts could not be separated from nucleotides by conventional desalting using gel filtration.