全文获取类型
收费全文 | 29149篇 |
免费 | 3689篇 |
国内免费 | 3篇 |
专业分类
32841篇 |
出版年
2021年 | 290篇 |
2018年 | 287篇 |
2017年 | 259篇 |
2016年 | 477篇 |
2015年 | 756篇 |
2014年 | 833篇 |
2013年 | 1068篇 |
2012年 | 1299篇 |
2011年 | 1199篇 |
2010年 | 773篇 |
2009年 | 743篇 |
2008年 | 1100篇 |
2007年 | 1087篇 |
2006年 | 1003篇 |
2005年 | 967篇 |
2004年 | 946篇 |
2003年 | 949篇 |
2002年 | 917篇 |
2001年 | 916篇 |
2000年 | 915篇 |
1999年 | 777篇 |
1998年 | 420篇 |
1997年 | 381篇 |
1996年 | 366篇 |
1995年 | 321篇 |
1994年 | 320篇 |
1993年 | 335篇 |
1992年 | 682篇 |
1991年 | 630篇 |
1990年 | 611篇 |
1989年 | 660篇 |
1988年 | 563篇 |
1987年 | 602篇 |
1986年 | 475篇 |
1985年 | 564篇 |
1984年 | 478篇 |
1983年 | 385篇 |
1982年 | 399篇 |
1981年 | 361篇 |
1980年 | 328篇 |
1979年 | 436篇 |
1978年 | 384篇 |
1977年 | 336篇 |
1976年 | 313篇 |
1975年 | 334篇 |
1974年 | 382篇 |
1973年 | 361篇 |
1972年 | 305篇 |
1971年 | 285篇 |
1969年 | 247篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
992.
T Smith 《BMJ (Clinical research ed.)》1979,1(6157):182-183
993.
N Smith A T Matheson M Yaguchi G E Willick R N Nazar 《European journal of biochemistry》1978,89(2):501-509
A 5-S RNA . protein complex has been isolated from the 50-S ribosomal subunit of an extreme halophile, Halobacterium cutirubrum. The 50-S ribosomal subunit from the extreme halophile requires 3.4 M K+ and 100 mM Mg2+ for stability. However, if the high K+ concentration is maintained but the Mg2+ concentration lowered to 0.3 mM, the 5-S RNA . protein complex is selectively extracted from the subunit. After being purified on an Agarose 0.5-m column the complex had a molecular weight of about 80000 and contained 5-S RNA and two proteins, HL13 and HL19, with molecular weights (by sedimentation equilibrium) of 18700 and 18000, respectively. No ATPase or GTPase activity could be detected in the 5-S RNA . protein complex. The amino acid composition and electrophoretic mobility on polyacrylamide gels indicated both proteins were much more acidic than the equivalent from Escherichia coli or Bacillus stearothermophilus. Partial amino acid sequence data suggest HL13 is homologous to EL18 and HL19 to EL5. 相似文献
994.
995.
996.
997.
Restoration of activity to catalytically deficient mutants of ribulosebisphosphate carboxylase/oxygenase by aminoethylation 总被引:3,自引:0,他引:3
Substitutions for active-site lysyl residues at positions 166 and 329 in ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum have been shown to abolish catalytic activity. Treatment of the Cys-166 and Cys-329 mutant proteins with 2-bromoethylamine partially restores enzyme activity, presumably as a consequence of selective aminoethylation of the thiol group unique to each protein. Amino acid analyses, slow inactivation of the wild-type carboxylase by bromoethylamine, and the failure of bromoethylamine to restore activity to the corresponding glycyl mutant proteins support this interpretation. The observed facile, selective aminoethylations may reflect an active site microenvironment not dissimilar to that of the native enzyme. Catalytic constants of these novel carboxylases, which contain a sulfur atom in place of a specific lysyl gamma-methylene group, are significantly lower than that of the wild-type enzyme. Furthermore, the aminoethylated mutant proteins form isolable complexes with a transition state analogue, but with compromised stabilities. These detrimental effects by such a modest structural change underscore the stringent requirement for lysyl side chains at positions 166 and 329. In contrast, the aminoethylated mutant proteins exhibit carboxylase/oxygenase activity ratios and Km values that are unperturbed relative to those for the native enzyme. 相似文献
998.
999.
1000.