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Six different bioassay methods were evaluated using propargite (Omite 30% wettable powder (WP) and fenbutatin oxide (Torque 50% (WP) and 55% suspension concentrate (SC)) with twospotted spider mite, Tetranychus uriticae Koch (TSM) and European red mite, Panonychus ulmi Koch (ERM) to document their utility and precision for estimating median lethal concentrations (LC). For each method, two post-treatment exposure periods and mortality criteria were used. Post-treatment exposure period and mortality criterion had a significant influence on the precision of LC50 estimates for all tested miticides with all bioassays methods. Twenty four hour (h) post-treatment exposure was found to be the most suitable for the slide dip and Petri dish methods while 48h was the most appropriate for leaf disc methods. Scoring moribund mites as dead was the most satisfactory criterion for ensuring that biossays were as simple and precise as possible. The Petri dish residue-Potter tower method (PDR-PT) estimated the responses of TSM and ERM to propargite with high precision. The same method was not as precise for fenbutatin oxide formulations. Because significant mite run-off occurred with the leaf disc methods, their precision was not fully established. The slide dip method gave less precise estimates of LC50 values for propargite (WP) and fenbutatin oxide (WP), while the same method gave more precise LC50 estimates for fenbutatin oxide (SC) than the PDR-PT method. The toxicity of candidate miticides was found to be method-and species-dependent. 相似文献
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R Jalleh DJ Torpy 《The Clinical biochemist. Reviews / Australian Association of Clinical Biochemists》2021,42(1):17
Direct measurement of the nonapeptide vasopressin has been limited by analyte instability ex vivo and in vivo rapid degradation, low serum concentrations requiring a sensitive assay and inherent secretory pulsatility. Copeptin is a 39 amino acid glycopeptide cleavage product of vasopressin synthesis with high stability, providing a marker of vasopressin secretion. Copeptin measurement has applications in diagnosis of diabetes insipidus and other diseases with altered vasopressin secretion. This review summarises our current understanding of serum copeptin measurement in diabetes insipidus and possible future applications of copeptin assays. As vasopressin is a stress hormone, there is emerging evidence on the use of copeptin for diagnosis and prognostication of disorders such as syndrome of inappropriate anti-diuretic hormone secretion, diabetes mellitus, critical illness, stroke, cardiovascular disease, respiratory disease, renal disease and thermal stress. Copeptin concentration measurement is likely to improve the diagnostic reliability of diabetes insipidus and, as a marker of stress, may have diagnostic or prognostic utility in specific clinical circumstances. Further studies are needed to determine if goal-directed therapy using plasma copeptin concentrations may improve patient outcomes. 相似文献
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