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by CLÉMENT COIFFARD BERNARD GOMEZ MÉLANIE THIÉBAUT JIÍ KVAEK† FRÉDÉRIC THÉVENARD DIDIER NÉRAUDEAU‡ 《Palaeontology》2009,52(2):323-336
Abstract: Eucalyptolaurus depreii gen. et sp. nov. is proposed for angiosperm leaves newly collected from uppermost Albian – lowermost Cenomanian of Charente-Maritime (western France). They consist of simple, narrow, elongate laminas with entire margins and intramarginal veins. The epidermal cells of adaxial cuticle shows small, rounded, blunt papillae outward that protrude inward and fuse together as rolls along and parallel to the margins, while the adaxial cuticle bears brachyparacytic stomatal apparatus that exhibit sunken guard cells and hair bases consisting of a thick-walled pore surrounded by radially arranged differentiated cells. Resin bodies occur inside the mesophyll. These characters closely resemble the lauroid taxa ' Myrtophyllum ' and Pandemophyllum from the Cenomanian of the Czech Republic and Dakota (USA) respectively. The narrow angle of basilaminar secondaries and the whole suite of features in the guard cells (sunken guard cells embedded into subsidiary cells and stomatal ledges) strongly support close affinity with the Lauraceae. From the Cenomanian lauraceous reproductive organs and their related leaves already showed high disparity and diversity. In addition they displayed a broad ecological range from freshwater floodplains to brackish swamps. This combined to high diversity of reproductive organs suggest ecological radiation of Lauraceae by the Cenomanian. 相似文献
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LINDA C. GREEN PETER J. DIDIER ELIZABETH S. DIDIER 《The Journal of eukaryotic microbiology》1999,46(4):434-438
Microsporidia are obligate intracellular parasites that are increasingly recognized as a cause of opportunistic infections in immunocompromised individuals. Encephalitozoon cuniculi has been identified in humans with AIDS and infects a wide range of mammalian hosts. Little is known about the metabolic processes that regulate growth and replication of microsporidia. Examination of the individual stages of development will facilitate such studies and reveal possible targets for drug therapy. The purpose of this study was to fractionate and purify stages of the microsporidian life cycle. Encephalitozoon cuniculi were cultured in RK-13 cells. The tissue supernatants containing multiple parasite stages, empty microsporidial husks and host cell debris were collected, washed, and subjected to differential centrifugation in 80% stock isotonic Percoll. Transmission electron microscopy and SDS-polyacrylamide gel electrophoresis were used to compare the content and purity of each fraction. Mature spores formed a band at a density of approximately 1.138 g/ml. Sporoblasts were found at densities between 1.102 g/ml and 1.119 g/ml. A mixture of sporonts, sporoblasts, microsporidial husks, and cell debris remained at the top of the gradient and additional centrifugation in 30% and 50% Percoll resulted in separation of these stages. These results represent the first step toward fractionating stages of microsporidia infecting humans. 相似文献
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MARIE‐NOËLLE VAULTIER MARIE‐PAULE HASENFRATZ‐SAUDER DIDIER LE THIEC PIERRE DIZENGREMEL GRAHAM NOCTOR YVES JOLIVET 《Plant, cell & environment》2013,36(11):1981-1991
Oxidative stress caused by ozone (O3) affects plant development, but the roles of specific redox‐homeostatic enzymes in O3 responses are still unclear. While growth day length may affect oxidative stress outcomes, the potential influence of day length context on equal‐time exposures to O3 is not known. In Arabidopsis Col‐0, day length affected the outcome of O3 exposure. In short‐days (SD), few lesions were elicited by treatments that caused extensive lesions in long days (LD). Lesion formation was not associated with significant perturbation of glutathione, ascorbate, NADP(H) or NAD(H). To investigate roles of two genes potentially underpinning this redox stability, O3 responses of mutants for cytosolic NADP‐isocitrate dehydrogenase (icdh) and glutathione reductase 1 (gr1) were analysed. Loss of ICDH function did not affect O3‐induced lesions, but slightly increased glutathione oxidation, induction of other cytosolic NADPH‐producing enzymes and pathogenesis‐related gene 1 (PR1). In gr1, O3‐triggered lesions, salicylic acid accumulation, and induction of PR1 were all decreased relative to Col‐0 despite enhanced accumulation of glutathione. Thus, even at identical irradiance and equal‐time exposures, day length strongly influences phenotypes triggered by oxidants of atmospheric origin, while in addition to its antioxidant function, the GR‐glutathione system seems to play novel signalling roles during O3 exposure. 相似文献
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The Truth from the Body: Medical Certificates as Ultimate Evidence for Asylum Seekers 总被引:1,自引:0,他引:1
Whether through traditional law or modern torture, the body has always been a privileged site on which to demonstrate the evidence of power. But for immigrants, the poor, and, more generally, the dominated—all of whom have to prove their eligibility to certain social rights—it has also become the place that displays the evidence of truth. In France, as immigration control increases, asylum seekers are more and more submitted to the evaluation of their physical sequels and psychic traumas, as if their autobiographical accounts were not sufficient. In this article, we show how nongovernmental organizations (NGOs) deal with the dilemmas posed by this situation, how they develop protocols standardizing their expertise, and how their medical authority progressively substitutes itself for the asylum seekers' word. In this process of objectification, it is the experience of the victims as political subjects that is progressively erased. 相似文献