Coevolution study of mitochondria respiratory chain proteins: toward the understanding of protein--protein interaction

Coevolution can be seen as the interdependency between evolutionary histories. In the context of protein evolution, functional correlation proteins are ever-present coordinated evolutionary characters without disruption of organismal integrity. As to complex system, there are two forms of protein-protein interactions in vivo, which refer to inter-complex interaction and intra-complex interaction. In this paper, we studied the difference of coevolution characters between inter-complex interaction and intra-complex interaction using "Mirror tree" method on the respiratory chain (RC) proteins. We divided the correlation coefficients of every pairwise RC proteins into two groups corresponding to the binary protein-protein interaction in intra-complex and the binary protein-protein interaction in inter-complex, respectively. A dramatical discrepancy is detected between the coevolution characters of the two sets of protein interactions (Wilcoxon test, p-value = 4.4 x 10-6). Our finding reveals some critical information on coevolutionary study and assists the mechanical investigation of protein-protein interaction.Furthermore, the results also provide some unique clue for supramolecular organization of protein complexes in the mitochondrial inner membrane. More detailed binding sites map and genome information of nuclear encoded RC proteins will be extraordinary valuable for the further mitochondria dynamics study.     

用于变应原疫苗分析和质控的实验技术

建立和应用一种可靠的质量分析方法是所有生物制品质量和工艺一致性的保障。一种准确反应药物中特异组成成分的活性和结构,并且优化和验证了的方法,是所有生物医药领域研发和新药上市的一个核心目标。变应原疫苗是天然物质的混合物,每一种变应原疫苗含有多种活性成分和与变应原无关未知的非活性成分,其中,具有变应原活性的物质只占一小部分,而且主要变应原成分作用在患者与患者之间差异性很大。本文介绍了一种变应原疫苗分析和质控的方法,包括变应原疫苗总蛋白含量、总蛋白组分、主要致敏蛋白组成、主要致敏蛋白含量、总生物活性几方面。     

2010年中国植物科学若干领域重要研究进展

2010年中国植物科学研究继续快速发展。中国科学家在植物科学各领域中取得了大量的具有原创意义的研究成果, 尤其是在水稻产量决定的遗传机制和作物基因组研究方面获得了一系列突破, 农业生态学研究等方面也取得了重大进展, 获得国内外的高度评价。该文对2010年中国本土植物生命科学若干领域取得的重要研究进展进行概括性评述, 旨在全面追踪当前中国植物科学领域发展的最新前沿和热点事件, 并展现我国科学家所取得的杰出成就。     

孕期应激对子代大鼠骨髓淋巴干细胞发育的影响

孕期应激对子代产生的影响是多方面的,这种影响是复杂的。研究表明,出生前的应激经历可导致出生后子代长期的免疫功能改变。这些改变追其根源与骨髓淋巴干细胞的改变有关。本文综述了大鼠孕期经历应激的子代骨髓淋巴干细胞所受的影响及免疫系统的相关改变,并根据现有的研究提出假说,为进一步研究孕期应激导致子代免疫系统改变的机理研究提供新的思路。     

利用黑曲霉β-葡萄糖苷酶催化香兰素葡萄糖苷水解

本文通过在香兰素葡萄糖苷溶液中加入黑曲霉菌种发酵制取的β-葡萄糖苷酶,进行酶促反应实验,定时取样进行高效液相分析检测,结果表明在反应过程中,溶液中香兰素葡萄糖苷的含量呈逐步下降趋势,香兰素的量呈逐步增加趋势;而在没有加β-葡萄糖苷酶的对照实验中,整个反应过程中香兰素葡萄糖苷的含量基本没有出现什么变化,在反应液中也没有检测到香兰素,这说明黑曲霉β-葡萄糖苷酶能够催化香兰素葡萄糖苷分解为香兰素的反应.     

重组人血小板生成素早期干预救治重症急性放射病猴的实验研究

重组人血小板生成素（rhTPO）是一种能促进巨核系祖细胞增殖、分化生成血小板的造血因子,研究表明它能促进射线照射小鼠造血功能恢复,前期工作证明rhTPO早期干预可显著提高致死剂量照射小鼠的活存率.本文以7.0Gy照射恒河猴为重度骨髓型急性放射病（ARS）模型,研究了rhTPO早期干预对重症ARS的治疗作用,并与WR2721和＂500＂的辐射防护作用进行了比较,结果发现rhTPO早期干预可明显促进ARS猴造血功能恢复,改善ARS猴症状,简化对症治疗措施,提高重度骨髓型ARS猴活存率,其对重度骨髓型ARS的防治作用优于现有的辐射防护药WR2721和＂500＂,有望开发成安全有效的新型辐射防治药物.     

水牛MyD88cDNA的克隆与原核表达

采用RT-PCR方法从水牛外周血白细胞总RNA中扩增出髓样分化因子88 (mydoid differentiation factor 88,MyD88) cDNA序列,PCR产物分离纯化后,与pMD20-T载体连接,重组质粒经PCR、酶切鉴定后测序,并进行生物信息学分析;构建pET28a-MyD88表达载体,并将其转化至E.coli BL21 (DE3),经IPTG诱导表达后,进行SDS-PAGE、镍柱亲和层析纯化和Western blotting分析.结果显示,克隆到的水牛MyD88 cDNA全长为1 189 bp,含有1个891 bp的开放阅读框,编码296个氨基酸,理论等电点为5.65.经IPTG诱导表达后,得到一个带His·Tag的约39 kD的重组融合蛋白.用抗His单克隆抗体进行Western blotting,得到1条约39 kD特异性抗体结合带,表明水牛MyD88原核表达载体成功构建并表达.本研究为进一步开展水牛MyD88的结构功能分析奠定了基础.     

Mental health system in China: history, recent service reform and future challenges

This paper summarizes the history of the development of Chinese mental health system; the current situation in the mental health field that China has to face in its effort to reform the system, including mental health burden, workforce and resources, as well as structural issues; the process of national mental health service reform, including how it was included into the national public health program, how it began as a training program and then became a treatment and intervention program, its unique training and capacity building model, and its outcomes and impacts; the barriers and challenges of the reform process; future suggestions for policy; and Chinese experiences as response to the international advocacy for the development of mental health.