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81.
Magdalena Nutu Birgitta Weijdegård Peter Thomas Ann Thurin-Kjellberg Håkan Billig DG Joakim Larsson 《Reproductive biology and endocrinology : RB&E》2009,7(1):89
Background
The controlled beating of cilia of the fallopian tube plays an important role in facilitating the meeting of gametes and subsequently transporting the fertilized egg to its implantation site. Rapid effects of progesterone on ciliary beat frequency have been reported in the fallopian tubes of cows, but the identity of the receptors mediating this non-genomic action of progesterone is not known. We recently identified a member of the non-genomic membrane progesterone receptor family, mPR gamma, as a candidate for mediating these actions of progesterone. Here, we investigated the possible presence of a related receptor, mPR beta, in the fallopian tubes of mice and women as well as the possible hormonal regulation of mPR beta and gamma. 相似文献82.
Damian DG Gessler Gary S Schiltz Greg D May Shulamit Avraham Christopher D Town David Grant Rex T Nelson 《BMC bioinformatics》2009,10(1):309-21
Background
SSWAP (Simple Semantic Web Architecture and Protocol; pronounced "swap") is an architecture, protocol, and platform for using reasoning to semantically integrate heterogeneous disparate data and services on the web. SSWAP was developed as a hybrid semantic web services technology to overcome limitations found in both pure web service technologies and pure semantic web technologies. 相似文献83.
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Ojovan SM Knorre DA Markova OV Smirnova EA Bakeeva LE Severin FF 《Journal of bioenergetics and biomembranes》2011,43(2):175-180
Hydrophobic cations with delocalized charge are used to deliver drugs to mitochondria. However, micromolar concentrations
of such compounds could be toxic due to their excessive accumulation in mitochondria. We studied possible pathophysiological
effects of one such cation, i.e. dodecyltriphenylphosphonium (C12-TPP), in the yeast Saccharomyces cerevisiae. First, we found that C12-TPP induces high-amplitude mitochondrial swelling. The swelling can be prevented by addition of protonophorous uncoupler
FCCP or antioxidant alpha-tocopherol, but not other tested antioxidants (N-acetylcysteine and Trolox). Second, FCCP prevents
ROS-sensitive fluorescent dye (dichlorofluorescein diacetate) staining of yeast treated with C12-TPP. We also showed that all tested antioxidants partially restore the growth inhibited by C12-TPP. The latter points that ROS rather than the mitochondria swelling limit the growth rate. 相似文献
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We present an updated account of breast cancer treatment and of progress toward “precision” cancer therapy; we focus on new developments in diagnostic molecular pathology and breast cancer that have emerged during the past 2 years. Increasing awareness of new prognostic and predictive methodologies, and introduction of next generation sequencing has increased understanding of both tumor biology and clinical behavior, which offers the possibility of more appropriate therapeutic choices. It remains unclear which of these testing methodologies provides the most informative and cost-effective actionable results for predictive and prognostic pathology. It is likely, however, that an integrated “step-wise” approach that uses the traditional clinical-pathologic paradigms coordinated with molecular characterization of breast tumor tissue, will offer the most comprehensive and cost-effective options for individualized, “precision” therapy for patients with breast cancer. 相似文献
88.
Yu. A. Mokrushina S. O. Pipiya A. V. Stepanova O. G. Shamborant V. D. Knorre I. V. Smirnov A. G. Gabibov I. I. Vorobiev 《Molecular Biology》2017,51(6):830-839
Catalytic antibodies are a promising model for creating highly specific biocatalysts with predetermined activity. However, in order to realize the directed change or improve their properties, it is necessary to understand the basics of catalysis and the specificity of interactions with substrates. In the present work, a structural and functional study of the Fab fragment of antibody A5 and a comparative analysis of its properties with antibody A17 have been carried out. These antibodies were previously selected for their ability to interact with organophosphorus compounds via covalent catalysis. It has been established that antibody A5 has exceptional specificity for phosphonate X with bimolecular reaction rate constants of 510 ± 20 and 390 ± 20 min–1M–1 for kappa and lambda variants, respectively. 3D-Modeling of antibody A5 structure made it possible to establish that the reaction residue L-Y33 is located on the surface of the active site, in contrast to the A17 antibody, in which the reaction residue L-Y37 is located at the bottom of a deep hydrophobic pocket. To investigate a detailed mechanism of the reaction, A5 antibody mutants with replacements L-R51W and H-F100W were created, which made it possible to perform stopped-flow kinetics. Tryptophan mutants were obtained as Fab fragments in the expression system of the methylotrophic yeast species Pichia pastoris. It has been established that the effectiveness of their interaction with phosphonate X is comparable to the wild-type antibody. Using the data of the stopped-flow kinetics method, significant conformational changes were established in the phosphonate modification process. The reaction was found to proceed using the induced-fit mechanism; the kinetic parameters of the elementary stages of the process have been calculated. The results present the prospects for the further improvement of antibody-based biocatalysts. 相似文献
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Investigation of activation of phosphate groups in mono- and oligonucleotides with mesitoyl chloride.
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V L Drutsa V F Zarytova D G Knorre A V Lebedev N I Sokolova Z A Shabarova 《Nucleic acids research》1978,5(1):185-193
It has been demonstrated with the use of 31P NMR pulsed spectroscopy that the reaction of mesitoyl chloride (MsCOCl) both with terminal and internucleotide phosphate groups pA, d(MeOTr)TpT and dpTpT (Ac) proceeds in a quantitative fashion within less than 2 min at 0 degrees C with the respective mixed anhydrides being thereby formed. The anhydrides of phosphomonoesters are resistant, unlike those of phosphodiesters which may be readily split by water, alcohol or amine without the internucleotide bonds being broken. Treatment of poly(U) with an excess of MsCOCl leads to rapid cyclization followed by formation of phosphotriesters. A comparatively easy hydrolysis leads to partial cleavage and isomerization of internucleotide bonds. A similar treatment of UpC showed that about 20% of the internucleotide bonds are cleaved, the remaining UpC being a mixture of approximately equal amounts of 3'-5'- and 2'-5'-isomers. 相似文献