全文获取类型
收费全文 | 118341篇 |
免费 | 146168篇 |
国内免费 | 35458篇 |
专业分类
299967篇 |
出版年
2021年 | 3025篇 |
2020年 | 2731篇 |
2019年 | 4875篇 |
2018年 | 4451篇 |
2017年 | 3922篇 |
2016年 | 4504篇 |
2015年 | 5084篇 |
2014年 | 6129篇 |
2013年 | 6167篇 |
2012年 | 7209篇 |
2011年 | 7448篇 |
2010年 | 7677篇 |
2009年 | 12421篇 |
2008年 | 6950篇 |
2007年 | 6640篇 |
2006年 | 5314篇 |
2005年 | 4860篇 |
2004年 | 4522篇 |
2003年 | 3931篇 |
2002年 | 4376篇 |
2001年 | 5368篇 |
2000年 | 2937篇 |
1999年 | 7338篇 |
1998年 | 9202篇 |
1997年 | 9339篇 |
1996年 | 8664篇 |
1995年 | 8958篇 |
1994年 | 8330篇 |
1993年 | 7949篇 |
1992年 | 7965篇 |
1991年 | 7964篇 |
1990年 | 8749篇 |
1989年 | 7989篇 |
1988年 | 7244篇 |
1987年 | 6388篇 |
1986年 | 5893篇 |
1985年 | 5309篇 |
1984年 | 4114篇 |
1983年 | 3314篇 |
1982年 | 3639篇 |
1981年 | 3282篇 |
1980年 | 3188篇 |
1979年 | 3305篇 |
1978年 | 3018篇 |
1977年 | 2937篇 |
1976年 | 2766篇 |
1974年 | 2499篇 |
1973年 | 2522篇 |
1972年 | 2855篇 |
1971年 | 2614篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
951.
Book reviewed in this article: Ecological theory and integrated pest management in practice. M. Kogan, editor World crop pests , Vol. 2. Aphids; their biology, natural enemies and control, PART A (Eds. A. K. Minks and P. Harrewijn) 相似文献
952.
Yu. Yu. Sharovskaja L. M. Chailakhjan L. B. Margolis 《Experimental cell research》1988,175(2):404-408
A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxically it is the standard fluorescent microscopy itself (that is, blue light of 320- to 480-nm wavelength) which induces rapid morphological alterations of cell culture followed by the transfer of fluorescent dye from one cell to another. Thus monitoring cell-cell dye coupling by fluorescent microscopy may itself induce the dye coupling in previously uncoupled epithelial cells. 相似文献
953.
The Maintenance of Single-Locus Polymorphism. I. Numerical Studies of a Viability Selection Model 总被引:5,自引:5,他引:0 下载免费PDF全文
The ability of viability selection to maintain single-locus polymorphism is investigated with two models in which the population is bombarded with a series of mutations with random fitnesses. In the first model, the population is allowed to reach equilibrium before mutation resumes; in the second the iterations and mutation occur simultaneously. Monte Carlo simulations of these models show that viability selection is easily able to maintain stable 6- or 7-allele polymorphisms and that monomorphisms and diallelic polymorphisms are uncommon. The question of how monomorphisms arise is also discussed. 相似文献
954.
955.
Molecular Characterization of the Mitochondrial DNA of a New Stopper Mutant Er-3 of Neurospora Crassa 总被引:4,自引:0,他引:4 下载免费PDF全文
An ethidium bromide-induced stopper mutant of Neurospora crassa is characterized at the molecular level. The mutant has two populations of mitochondrial DNA: a defective predominant mutant molecule and a basal level of the wild-type molecule. The aberrant DNA resulted after a 25-kbp deletion from the wild-type mitochondrial chromosome, which included major genes such as cytb, co1 and oli2. The deletion endpoints are located in the second intron of the ND5 gene, and in a sequence 250 nucleotides upstream of the co2 gene. The recombination has taken place between two nine nucleotide repeats CCCCGCCCC, one of which is close to a PstI palindrome at its 5' end. Thus the mutant ER-3 differs from all the other stopper mutants described previously in the extent and location of the deletions in the mtDNA. 相似文献
956.
Paternal Imprinting of Inversion Uab(1) Causes Homeotic Transformations in Drosophila 总被引:1,自引:0,他引:1 下载免费PDF全文
Paternal transmission of the bithorax-complex (BX-C) rearrangement, inversion Uab(1), causes a specific dominant gain of function phenotype in most abdominal segments. This represents a case of paternal imprinting since the mutant phenotype will occur only if inversion Uab(1) is paternally transmitted. The transformations in males are toward genital arch tissue. For females the transformations are to tissue found on abdominal segment 7 (Ab7) and to structures normally restricted to the genital disc. Ninety-six percent of transformed areas appear on Ab5 and Ab6 in both sexes and on Ab7 in females, coinciding with the Abd-B domain. Four percent of the transformations occurred on Ab1 through Ab4, coinciding with the abd-A domain. The mutant phenotype can be dramatically enhanced by modifying genes such as the posterior BX-C mutant tuh-3. Expressivity is modulated by maternal effect alleles interacting with tuh-3. A region of function within inversion Uab(1) appears to be programmed during spermatogenesis to function in a legacy dependent manner during embryogenesis. 相似文献
957.
Gene organization in the region containing a new gene involved in chromosome partition in Escherichia coli. 总被引:21,自引:9,他引:12 下载免费PDF全文
A new mutation, parC, causing abnormal chromosome segregation was identified in two thermosensitive mutants of Escherichia coli. The thermosensitive growth of the mutants was corrected by pLC4-14 in the Clarke-Carbon collection. This plasmid carries a putative gene which can suppress the cell division defect due to ftsI (pbpB) and has hence been termed sufI (sui). The nearness of parC to metC was confirmed, and cotransduction frequency of parC was 59% with metC and 20% with glc. The parC-sufI region was analyzed by subcloning the chromosome region of pLC4-14. The parC and the sufI gene products were electrophoretically identified as proteins of 75 and 55 kilodaltons (kDa), respectively. The allelism of parC+ on pLC4-14 to parC1215 was confirmed by cloning parC1215. The sufI gene appeared to be dispensable for cell viability, and overproduction of its product caused suppression of ftsI. An essential gene coding for a 25-kDa protein was found between the parC and the sufI gene. These three genes were transcribed in the same direction and may be organized into an operon, with parC to the proximal side and with internal promoters at least for the distal genes. The localization of the gene products was examined in maxicells. The sufI protein was synthesized as a precursor which could be chased into a mature form. The major part of the mature form was found in the soluble fraction. The 25-kDa protein was found almost exclusively in the membrane fraction. The parC protein was associated with the membrane fraction in the presence of Mg2+ but found in the soluble fraction when Mg2+ was sequestered with EDTA. 相似文献
958.
Characterization of the Pseudomonas aeruginosa recA gene: the Les- phenotype. 总被引:3,自引:2,他引:1 下载免费PDF全文
The Les- phenotype (lysogeny establishment deficient) is a pleiotropic effect of the lesB908 mutation of Pseudomonas aeruginosa PAO. lesB908-containing strains are also (i) deficient in general recombination, (ii) sensitive to UV irradiation, and (iii) deficient in UV-stimulated induction of prophages. The P. aeruginosa recA-containing plasmid pKML3001 complemented each of these pleiotropic characteristics of the lesB908 mutation, supporting the hypothesis that lesB908 is an allele of the P. aeruginosa recA gene. The phenotypic effects of the lesB908 mutation may be best explained by the hypothesis that the lesB908 gene product is altered in such a way that it has lost synaptase activity but possesses intrinsic protease activity in the absence of DNA damage. The Les- phenotype is a result of the rapid destruction of newly synthesized phage repressor, resulting in lytic growth of the infecting virus. This hypothesis is consistent with the observations that increasing the number of copies of the phage repressor gene by increasing the multiplicity of infection (i.e., average number of phage genomes per cell) or by introducing the cloned phage repressor gene into a lesB908 mutant will also suppress the Les- phenotype in a phage-specific fashion. 相似文献
959.
Chemotaxis of Rhizobium meliloti to the plant flavone luteolin requires functional nodulation genes. 总被引:23,自引:5,他引:18 下载免费PDF全文
Luteolin is a phenolic compound from plants that acts as a potent and specific inducer of nodABC gene expression in Rhizobium meliloti. We have found that R. meliloti RCR2011 exhibits positive chemotaxis towards luteolin. A maximum chemotactic response was observed at 10(-8) M. Two closely related flavonoids, naringenin and apigenin, were not chemoattractants. The presence of naringenin but not apigenin abolished chemotaxis of R. meliloti towards luteolin. A large deletion in the nif-nod region of the symbiotic megaplasmid eliminated all chemotactic response to luteolin but did not affect general chemotaxis, as indicated by swarm size on semisoft agar plates and chemotaxis towards proline in capillary tubes. Transposon Tn5 mutations in nodD, nodA, or nodC selectively abolished the chemotactic response of R. meliloti to luteolin. Agrobacterium tumefaciens GMI9050, a derivative of the C58 wild type lacking a Ti plasmid, responded chemotactically to 10(-8) M luteolin. The introduction of a 290-kilobase nif-nod-containing sequence of DNA from R. meliloti into A. tumefaciens GMI9050 enabled the recipient to respond to luteolin at concentrations peaking at 10(-6) M as well as at concentrations peaking at 10(-8) M. The response of A. tumefaciens GMI9050 to luteolin was also abolished by the presence of naringenin. 相似文献
960.
The cryptic bgl operon in Escherichia coli K-12 strain 1011A contains a 1.4-kilobase-pair fragment of foreign DNA within the bglF structural gene. The active allele found in its descendant strain, MK1, required the precise excision of that insertion for its activation. Molecular and genetic approaches have shown that strain 1011A possessed an active (bglR+) rather than a silent wild-type (bglR0) allele of the regulatory region and that this change was caused by a point mutation. Our model for the retention of cryptic genes (B. G. Hall, S. Yokoyama, and D. H. Calhoun, Mol. Biol. Evol. 1:109-124, 1983) suggested that the insertion might have been selected to silence a disadvantageous bglR+ allele. We examined the genealogy of strain MK1 and found that the insertion of foreign DNA was not selected for that reason, since it preceded the change to bglR+. This means that the change to bglR+ was also not selected, since the presence of the insertion would not allow expression of the operon. We have calculated the probability of isolating a bglR+ mutation by chance alone as less than 10(-8). We suggest that mutation rates estimated under the usual conditions of exponential growth may be irrelevant to the frequencies of these events under natural conditions. 相似文献