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941.
942.
特异切割马铃薯纺锤形块茎类病毒负链的多价核酶的构建和体外活性测定 总被引:4,自引:0,他引:4
根据锤头型核酶的作用模式 ,设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒 (PSTVd)负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录 ,将PSTVd负链RNA分别与双价和三价核酶混合 ,37℃温育 2h。结果表明 ,双价核酶和三价核酶均表现出较高的切割活性 ,其中双价核酶处理的切割产物的大小与理论值相符合。三价核酶虽表现出较高的切割活性 ,但只是其中一价核酶在起作用。讨论了二价和三价核酶的应用前景。 相似文献
943.
944.
视黄醇结合蛋白的结构与功能 总被引:7,自引:0,他引:7
金宏 《生物化学与生物物理进展》1996,23(2):126-129
视黄醇结合蛋白(RBP)是视黄醇转运的载体蛋白,作为结合小分子流水物质的载体蛋白家族(lipocalin)的一个重要成员,其结构与功能的研究正受到国外学者的重视,文章介绍了视黄醇结合蛋白的性质、结构研究进展,讨论了视黄醇结合蛋白与前蛋白和受体相互作用的位点和结构特点. 相似文献
945.
用PEG—高Ca高PH法诱导抗卡那霉素的烟草(Nicotianatabacum)品系N364+Km+花粉原生质体和黄花烟草(Nicotiarustica)叶肉原生质体融合。幼嫩花粉原生质体和叶肉原生质体之间的融合体培养启动胚胎发生分裂,经卡那霉素筛选后,少数多细胞团存活并形成小愈伤组织。成熟花粉原生质体与叶肉原生质体之间的融合体则仅产生管状结构。这一结果表明,作为融合一方的花粉原生质体的发育时期对融合产物的发育途径有重要影响。 相似文献
946.
梅花鹿甲烷能代谢规律的研究 总被引:1,自引:1,他引:1
本文应用KB-1型呼吸测热装置,结合消化、代谢试验,对梅花鹿(Cervusnippon)甲烷能代谢规律进行了研究。结果表明,梅花鹿甲烷能的产生量随其采食量的增加而增加;也随着果食后时间的推移而减少,而且减少的幅度又随采食量的增加而下降;甲烷能的产生量分别占总能食入量、消化能食入量和体增热的6.61%、8.83%和10.88%;甲烷能的产生量随着日粮蛋白质水平的提高而降低,日粮蛋白质水平每提高1个百分点,甲烷能产生量就降低58.58kJ/d;分别以总能食入量(GEI)和干物质食入量(DMI)为自变量所建立的甲烷能(CH4E)估计分别为:CH4E(kJ/d)=0.07CEJ(kJ/d)-101.04(n=12,r=0.944,P<0.01)CH4E(kJ/d)=98.78+1.05DMI(g/d)(n=12,r=0.942,P<0.01) 相似文献
947.
Purification and characterization of catabolic mannopine cyclase encoded by the Agrobacterium tumefaciens Ti plasmid pTi15955. 下载免费PDF全文
Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography. The enzyme catalyzed the lactonization of MOP to AGR without the need for any cofactors. The enzyme also converted AGR to MOP with the lactonizing activity being predominant over the reverse reaction. MOP cyclase is specific for imine conjugates of D-hexose and L-glutamine and was not inhibited by sugars or amino acids. The enzyme lactonized deoxyfructosyl glutamine, a natural intermediate of MOP synthesis and catabolism, to a product indistinguishable from chrysopine, a newly discovered crown gall opine. The enzyme also lactonized N-l-(1,2-dideoxy-D-mannityl)-L-glutamine, indicating that a hydroxyl group at carbon atom 2 of the sugar moiety is not required for the enzymatic reaction. 相似文献
948.
949.
Xiahui Zhu Hong Zhang Tamo Fukamizo S. Muthukrishnan Karl J. Kramer 《Insect biochemistry and molecular biology》2001,31(12)
Manduca sexta (tobacco hornworm) chitinase is a molting enzyme that contains several domains including a catalytic domain, a serine/threonine-rich region, and a C-terminal cysteine-rich domain. Previously we showed that this chitinase acts as a biopesticide in transgenic plants where it disrupts gut physiology. To delineate the role of these domains further and to identify and characterize some of the multiple forms produced in molting fluid and in transgenic plants, three different forms with variable lengths of C-terminal deletions were generated. Appropriately truncated forms of the M. sexta chitinase cDNA were generated, introduced into a baculovirus vector, and expressed in insect cells. Two of the truncated chitinases (Chi 1-407 and Chi 1-477) were secreted into the medium, whereas the one with the longest deletion (Chi 1-376) was retained inside the insect cells. The two larger truncated chitinases and the full-length enzyme (Chi 1-535) were purified and their properties were compared. Differences in carbohydrate compositions, pH–activity profiles, and kinetic constants were observed among the different forms of chitinases. All three of these chitinases had some affinity for chitin, and they also exhibited differences in their ability to hydrolyze colloidal chitin. The results support the hypothesis that multiple forms of this enzyme occur in vivo due to proteolytic processing at the C-terminal end and differential glycosylation. 相似文献
950.
C S Hong Y G Kwak J H Ji S W Chae Do H Kim 《Biochemical and biophysical research communications》2001,289(4):882-887
Junctate is a newly identified integral ER/SR membrane calcium binding protein, which is an alternative splicing form of the same gene generating aspartyl beta-hydroxylase and junctin. Screening a mouse heart cDNA library using canine junctin cDNA as a probe yielded three complete mouse heart cDNAs. One of the cDNAs is homologous to the previously reported human junctate. The three mouse junctate proteins are composed of 270, 259, and 215 amino acids (we named them junctate-1, -2, and -3). The apparent molecular masses of the mouse junctates in SDS-PAGE were in the range between 40 and 53 kDa. Northern and Western blot analyses indicate that mouse junctates are expressed in heart, brain, spleen, lung, liver, kidney, and stomach, but not in skeletal muscle. The apparent molecular weights of junctates from heart and brain were somewhat different from those from the other tissues tested, suggesting that there are tissue-specific expression patterns of the different junctate isoforms. Immunohistochemical studies showed that junctates were expressed both in ventricular and atrial tissues. This is the first study that shows the presence of 3 distinct cardiac junctate isoforms expressed in various mammalian tissues. 相似文献