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981.
【背景】拉鲁湿地是我国海拔最高、面积最大的城市天然湿地。开展拉鲁湿地微生物群落结构分析,可为青藏高原生物资源的开发利用和保护提供理论依据,并为湿地生态系统的微生物多样性研究奠定基础。【目的】开展拉鲁湿地水体丝状真菌的多样性研究,探究影响该环境丝状真菌群落分布特征的主要理化因子。【方法】从拉鲁湿地11个水样中分离丝状真菌,应用nr DNA ITS序列分析,并结合经典分类学方法对获得的菌株进行鉴定,运用SPSS和CANOCO软件分析丝状真菌群落结构及其与环境因子的关系。【结果】从拉鲁湿地水体分离的丝状真菌菌株分属于6属13种。拉鲁湿地冬季水体丝状真菌优势属为Mucor、Cladosporium和Galactomyces,优势种为M.hiemalis、M.racemosus和G.geotrichum。总氮与拉鲁湿地水体丝状真菌数量呈显著负相关(P0.05),总氮和总磷对于丝状真菌的分布具有较明显的影响,这2种理化因子与M.racemosus的多度呈正相关性,而与M.hiemalis呈较强的负相关性。【结论】环境因子是影响高原湿地生态系统微生物群落结构的主要因素之一,探讨西藏典型脆弱生态环境中微生物多样性与环境因子的关系具有重要意义。 相似文献
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Four African species of true water bugs (Nepomorpha: Hemiptera: Heteroptera) are studied by mass spectrometry and biological assays to gain information on the presence, structure and function of peptides from the adipokinetic hormone (AKH) family, which are produced in the corpora cardiaca (CC). The water scorpion Laccotrephes fabricii Stål (Nepidae) has the peptide code‐named Peram‐CAH‐I with the sequence pGlu‐Val‐Asn‐Phe‐Ser‐Pro‐Asn‐Trp amide, whereas Appasus grassei Poisson (Belostomatidae) produces Anaim‐AKH, which is a Ser7 analogue of Peram‐CAH‐I (pGlu‐Val‐Asn‐Phe‐Ser‐Pro‐Ser‐Trp amide). The giant water bug Hydrocyrius columbiae Spinola (Belostomatidae) has two adipokinetic hormone family members: Anaim‐AKH and Letin‐AKH, which again differ only at position 7 (Ser7 versus Tyr7). When the sequence data are compared with current molecular phylogenetic analyses of Nepomorpha, they are essentially in agreement with the newest ideas on phylogenetic relationships among the families. Functional investigation of these peptides reveals a mainly lipid‐based energy metabolism in these insects, as demonstrated by a hyperlipaemic response after injecting crude CC extract or the appropriate peptide into the respective species. The carbohydrate concentration in the haemolymph is not affected by such injections, and the carbohydrate level in most cases is lower than that of the circulating lipids. During physical exercise, such as swimming for 1 h, carbohydrates may contribute to some extent to the provision of energy; the substantial increase in the concentration of lipids in the haemolymph, however, is a strong indicator that the peptides are released from the CC and act primarily as true adipokinetic hormones during this period of intense muscular activity. 相似文献
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CARLOS PEREIRA DE BRITO 《Biological journal of the Linnean Society. Linnean Society of London》1992,46(1-2):145-151
Oxychilus (Drouetia) atlanticus is an endemic terrestrial mollusc from the Azores that shows a high degree of both anatomical and morphological variability. In order to support a biochemical systematic study, several potential taxonomic isoenzymes were tested over non-congeneric, congeneric, consubgeneric and infraspecific taxa. 相似文献
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MARIANA MEERHOFF CARLOS IGLESIAS FRANCO TEIXEIRA DE MELLO JUAN M. CLEMENTE ELISABETH JENSEN TORBEN L. LAURIDSEN ERIK JEPPESEN 《Freshwater Biology》2007,52(6):1009-1021
1. Structural complexity may stabilise predator–prey interactions and affect the outcome of trophic cascades by providing prey refuges. In deep lakes, vulnerable zooplankton move vertically to avoid fish predation. In contrast, submerged plants often provide a diel refuge against fish predation for large‐bodied zooplankton in shallow temperate lakes, with consequences for the whole ecosystem. 2. To test the extent to which macrophytes serve as refuges for zooplankton in temperate and subtropical lakes, we introduced artificial plant beds into the littoral area of five pairs of shallow lakes in Uruguay (30°–35°S) and Denmark (55°–57°N). We used plants of different architecture (submerged and free‐floating) along a gradient of turbidity over which the lakes were paired. 3. We found remarkable differences in the structure (taxon‐richness at the genus level, composition and density) of the zooplankton communities in the littoral area between climate zones. Richer communities of larger‐bodied taxa (frequently including Daphnia spp.) occurred in the temperate lakes, whereas small‐bodied taxa characterised the subtropical lakes. More genera and a higher density of benthic/plant‐associated cladocerans also occurred in the temperate lakes. The density of all crustaceans, except calanoid copepods, was significantly higher in the temperate lakes (c. 5.5‐fold higher). 4. Fish and shrimps (genus Palaemonetes) seemed to exert a stronger predation pressure on zooplankton in the plant beds in the subtropical lakes, while the pelagic invertebrate Chaoborus sp. was slightly more abundant than in the temperate lakes. In contrast, plant‐associated predatory macroinvertebrates were eight times more abundant in the temperate than in the subtropical lakes. 5. The artificial submerged plants hosted significantly more cladocerans than the free‐floating plants, which were particularly avoided in the subtropical lakes. Patterns indicating diel horizontal migration were frequently observed for both overall zooplankton density and individual taxa in the temperate, but not the subtropical, lakes. In contrast, patterns of diel vertical migration prevailed for both the overall zooplankton and for most individual taxa in the subtropics, irrespective of water turbidity. 6. Higher fish predation probably shapes the general structure and dynamics of cladoceran communities in the subtropical lakes. Our results support the hypothesis that horizontal migration is less prevalent in the subtropics than in temperate lakes, and that no predator‐avoidance behaviour effectively counteracts predation pressure in the subtropics. Positive effects of aquatic plants on water transparency, via their acting as a refuge for zooplankton, may be generally weak or rare in warm lakes. 相似文献