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141.
Summary In the Epiponini, queen number may vary from many to few during the colonial cycle. This cyclical oligogyny may be important for the maintenance of high genetic relatedness found in several taxa of epiponines. Even though queen reduction in accord with the colony cycle has either been demonstrated or inferred for many taxa, the factors responsible for queen number reduction remain largely unknown. In this study, behavioral interactions (queen-queen and queen-worker) were analyzed in Asteloeca ujhelyii. Conflicts among queens leading to queen elimination were more intense during colony establishment, and queens behavioral performances were significantly correlated with their potential reproductive capacity. Frequency of workers testing behaviors toward queens did not relate as a function of the colony cycle. Thus, it seems that in Asteloeca ujhelyii cyclical oligogyny is indeed behaviorally mediated.Received 16 January 2003; revised 1 June 2003; accepted 3 July 2003. 相似文献
142.
Genotoxicity of diphenyl diselenide in bacteria and yeast 总被引:2,自引:0,他引:2
Rosa RM Sulzbacher K Picada JN Roesler R Saffi J Brendel M Henriques JA 《Mutation research》2004,563(2):107-115
Diphenyl diselenide (DPDS) is an electrophilic reagent used in the synthesis of a variety of pharmacologically active organic selenium compounds. This may increase the risk of human exposure to the chemical at the workplace. We have determined its mutagenic potential in the Salmonella/microsome assay and used the yeast Saccharomyces cerevisiae to assay for putative genotoxicity, recombinogenicity and to determine whether DNA damage produced by DPDS is repairable. Only in exponentially growing cultures was DPDS able to induce frameshift mutations in S. typhimurium and haploid yeast and to increase crossing over and gene conversion frequencies in diploid strains of S. cerevisiae. Thus, DPDS presents a behavior similar to that of an intercalating agent. Mutants defective in excision-resynthesis repair (rad3, rad1), in error-prone repair (rad6) and in recombinational repair (rad52) showed higher than WT-sensitivity to DPDS. It appears that this compound is capable of inducing single and/or double strand breaks in DNA. An epistatic interaction was shown between rad3-e5 and rad52-1 mutant alleles, indicating that excision-resynthesis and strand-break repair may possess common steps in the repair of DNA damage induced by DPDS. DPDS was able to enhance the mutagenesis induced by oxidative mutagens in bacteria. N-acetylcysteine, a glutathione biosynthesis precursor, prevented mutagenesis induced by DPDS in yeast. We have shown that DPDS is a weak mutagen which probably generates DNA strand breaks through both its intercalating action and pro-oxidant effect. 相似文献
143.
144.
Martinez ML Machado MA Nascimento CS Silva MV Teodoro RL Furlong J Prata MC Campos AL Guimarães MF Azevedo AL Pires MF Verneque RS 《Genetics and molecular research : GMR》2006,5(3):513-524
Losses caused by bovine tick burdens in tropical countries have a tremendous economic impact on production systems. Besides reducing production, this parasite can cause death in the most susceptible animals. The use of commercial acaricides has been the major method of control, but their misuse has led to tick resistance to many chemicals. More recently, vaccines have been used in some countries without solving the problem completely. An alternative could be the development of resistant animals and the use of genetic markers and candidate genes that could help with the enormous task of selecting resistant animals. The bovine lymphocyte antigen genes (BoLA) have been shown to be associated with some parasitic infestations and disease incidence. Thus, the objective of the present study was to determine the association of BoLA-DRB3.2 alleles with tick resistance in cattle. The study was conducted on 231 F2 (Gyr x Holstein) animals that were artificially infested with 10,000 tick larvae. Log of tick count +1 was used as the dependent variable in a mixed animal model with allele substitution effects in addition to fixed effects of year and season at tick count, sex of calves, age of animal at tick count, hair type (short-straight, short-curl, long-straight, and long-curl), coat color (white, >75% white, 50- 75% white, and 25-50% white), and additive genetic, permanent environmental and residual effects as random. Females showed fewer ticks than males. Animals with short-straight hair were more resistant to tick infestation than animals with long-curl hair, and animals with whiter coat color also had fewer ticks. An association between BoLA alleles and lower tick number was found for alleles DRB3.2 *18, *20 and *27 at the 5% significance level. Also, one allele (DRB3.2*16) showed an association at the 10% level. Allele *27 was the most frequent in the population (30.7%), followed by alleles *16 (10.8%), *20 (8.7%) and *18 (2.4%). These results suggest that BoLA-DRB3.2 alleles could be used to help in the selection of animals resistant to tick infestation. However, further studies involving a larger population of cattle in combination with other BoLA genes may help to understand the mechanisms of resistance to parasites. 相似文献
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146.
Valdirene Neves Monteiro Roberto do Nascimento Silva Andrei Stecca Steindorff Fabio Teles Costa Eliane Ferreira Noronha Carlos André Ornelas Ricart Marcelo Valle de Sousa Marilene Henning Vainstein Cirano José Ulhoa 《Current microbiology》2010,61(4):298-305
Trichoderma harzianum ALL42 were capable of overgrowing and degrading Rhizoctonia solani and Macrophomina phaseolina mycelia, coiling around the hyphae with formation of apressoria and hook-like structures. Hyphae of T. harzianum ALL42 did not show any coiling around Fusarium sp. hyphae suggesting that mycoparasitism may be different among the plant pathogens. In this study, a secretome analysis
was used to identify some extracellular proteins secreted by T. harzianum ALL42 after growth on cell wall of M. phaseolina, Fusarium sp., and R. solani. The secreted proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. A total of 60 T. harzianum ALL42 secreted proteins excised from the gel were analyzed from the three growth conditions. While seven cell wall-induced
proteins were identified, more than 53 proteins spots remain unidentified, indicating that these proteins are either novel
proteins or proteins that have not yet been sequenced. Endochitinase, β-glucosidase, α-mannosidase, acid phosphatase, α-1,3-glucanase,
and proteases were identified in the gel and also detected in the supernatant of culture. 相似文献
147.
Studies of ant–plant relationships elucidate how top-down effects of the third trophic level can affect the biomass, richness, and/or species composition of plants. Although widespread in the neotropics, few studies have so far examined the direct effects of ants on plant fitness. Here, through experimental manipulation (ant-exclusion) under natural conditions, we examined the effect of ant visitation to extrafloral nectaries on leaf herbivory and fruit set in Chamaecrista debilis in the Brazilian savanna. As opposed to other Chamaecrista species, our results showed that visiting ants (15 species) significantly reduce herbivory and increase fruit set by more than 50% compared to plants from which ants were excluded. This mutualistic system is facultative in nature, and corroborates the potential beneficial role of exudate-feeding ants as anti-herbivore agents of tropical plants. 相似文献
148.
Juliana Maria Motta Clarissa Rodrigues Nascimento Vivian Mary Rumjanek 《Cancer immunology, immunotherapy : CII》2010,59(11):1645-1653
The microenvironment produced by solid tumors is inhibitory to the immune system, inducing dendritic cell (DC) alterations,
but there is a paucity of information regarding haematological malignances. The aim of this study was to investigate DC differentiation
under the influence of leukemic cell products. Monocytes from healthy volunteers were cultured in the presence of IL-4 and
GM-CSF for the generation of immature DCs. Supernatants from leukemic cultures were added to monocyte cultures during differentiation.
The lineages used were K562, a chronic myeloid leukemia, HL-60, a promyelocytic leukemia and DAUDI, originated from Burkitt
lymphoma. It was observed that the expression of CD14 remained high and the CD1a was low in the presence of tumor supernatants,
while non-malignant supernatants did not affect these parameters. Furthermore, IL-1β and TNF-α production by monocytes during
differentiation was increased by the presence of tumor supernatants. The modifications on CD14 and CD1a expressions could
be mimicked by the addition of exogenous IL-1β and partially inhibited by the neutralization of IL-1β. These results suggest
that soluble products from leukemic cells interfere with DC differentiation and, in the present work, this effect could be
mediated by monocyte-derived IL-1β in response to tumor supernatants. 相似文献
149.
Joana Rocha-Pereira Ricardo Cunha Diana C.G.A. Pinto Artur M.S. Silva Maria São José Nascimento 《Bioorganic & medicinal chemistry》2010,18(12):4195-4201
Human noroviruses (NoV) are now recognized as the most frequent cause of outbreaks and sporadic cases of acute gastroenteritis. Despite the significant economic impact and considerable morbidity of norovirus disease, no drug or vaccine is currently available to treat or prevent this disease, therefore the discovery of anti-norovirus drugs is urgent.In the present work, a total of 12 structure related chromone and (E)-2-styrylchromones were evaluated for their potential anti-norovirus activity using the murine norovirus (MNV) as a surrogate model for human NoV.From the 12 compounds studied, six (E)-2-styrylchromones were found to have with interesting anti-norovirus activity. The best compounds of the series were (E)-5-hydroxy-2-styrylchromone and (E)-4′-methoxy-2-styrylchromone with an IC50 ≈ 7 μM. A first insight into the mechanism of action of these compounds was possible. An interesting relationship between the anti-norovirus activity and the chemical structure was observed. The present study points out that the (E)-2-styrylchromones skeleton is an important one which deserves to be developed and further explored as new antiviral drugs against NoV. 相似文献