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161.
JENNIFER M. BLAIR BRENDAN J. HICKS ROBERT J. PITKETHLEY NICHOLAS LING ILIA OSTROVSKY DAVID K. ROWE 《Freshwater Biology》2013,58(5):967-985
Summary
- 1 To investigate the carrying capacity and factors affecting growth of rainbow trout in Lake Rotoiti, we employed a bioenergetics model to assess the influence of stocking rates, timing of releases and prey abundance on growth and prey consumption. We hypothesised that stocking rates and prey abundance would affect growth and prey consumption by influencing per‐capita prey availability, and that the environmental conditions encountered by fish at the time of stocking would affect growth and consumption.
- 2 Prey consumption of stocked rainbow trout was calculated with the Wisconsin bioenergetics model. We calculated growth trajectories of released trout based on data from stocked trout that were released in spring and autumn from 1993 to 2009 and then re‐captured by anglers. Diet, prey energy density, body mass lost during spawning and lake temperature were measured locally.
- 3 Stocking timing had no effect on return rates to anglers or length or weight of caught fish. Although trout released in autumn were smaller than those released in spring, autumn‐released trout grew at a faster rate and had similar lengths and weights to spring cohorts after 2 years of growth in the lake. Modelled consumption parameters were negatively correlated with trout population size, suggesting that stocking rates (347–809 fish ha?1 year?1) caused density‐dependent effects on growth. Although common smelt (Retropinna retropinna) accounted for 85% of total prey consumption, no significant relationship was found between prey consumption by individual trout and adult smelt abundance, possibly because trout are targeting smaller smelt that our abundance estimate did not account for.
- 4 Releasing trout in autumn appears to be advantageous for growth, possibly because (i) temperature is more suitable for growth in autumn–winter than in spring–summer and (ii) prey for small trout is abundant in autumn. Mild winter conditions appear to enhance overwinter survival and growth of rainbow trout in warm‐temperate lakes compared to higher latitudes. This implies that moderately productive warm‐temperate lake ecosystems are highly suitable for trout growth in winter, but less so in summer, when lake stratification and high nutrient levels may create conditions suitable for algal blooms and hypolimnetic deoxygenation. High growth rates of trout in warm‐temperate lakes can therefore be supported by timing releases to coincide with favourable winter conditions.
162.
ABSTRACT Attraction of flying mole crickets to individual males calling in an outdoor arena was influenced significantly by the relative intensity of the males calling in the arena. Louder males attracted more individuals than males whose calling songs were less intense. Making simple assumptions about the acoustic output from calling males and about the flight pattern and response of flying females, the differential attraction can be explained by a mathematical model. Computer simulation of the model was used to examine the importance of a male's intensity relative to others and the effect of distance between males on the attraction of females. The model and its relation to active female choice and passive attraction are discussed. The model makes predictions about differences in spacing behaviour of males that maximize attraction relative to other males. 相似文献
163.
JAMES W. BORRONE J. STEVEN BROWN DAVID N. KUHN JUAN C. MOTAMAYOR RAYMOND J. SCHNELL 《Molecular ecology resources》2007,7(2):236-239
Theobroma cacao L. expressed sequence tags (ESTs) were converted into useful genetic markers for fingerprinting individuals and genetic linkage mapping. Primers were designed to microsatellite‐containing ESTs. Twenty‐two T. cacao accessions, parents of various mapping populations segregating for disease resistance and crop yield characteristics, were tested. Twenty‐seven informative loci were discovered with 26 primer pairs. The number of detected alleles ranged from two to 11 and averaged 4.4 per locus. All 27 markers could be mapped into at least one of the existing F1 or F2 populations segregating for agronomically important traits. 相似文献
164.
DAVID E. RICHARDSON JEFFREY D. VANWYE AMY M. EXUM ROBERT K. COWEN DOUGLAS L. CRAWFORD 《Molecular ecology resources》2007,7(2):199-207
Ichthyoplankton collections provide a valuable means to study fish life histories. However, these collections are greatly underutilized, as larval fishes are frequently not identified to species due to their small size and limited morphological development. Currently, there is an effort underway to make species identification more readily available across a broad range of taxa through the sequencing of a standard gene. This effort requires the development of new methodologies to both rapidly produce and analyse large numbers of sequences. The methodology presented in this paper addresses these issues with a focus on the larvae of large pelagic fish species. All steps of the methodology are targeted towards high‐throughput identification using small amounts of tissue. To accomplish this, DNA isolation was automated on a liquid‐handling robot using magnetic bead technologies. Polymerase chain reaction and a unidirectional sequencing reaction followed standard protocols with all template cleanup and transferring also automated. Manual pipetting was thus reduced to a minimum. A character‐based bioinformatics program was developed to handle the large sequence output. This program incorporates base‐call quality scores in two types of sample to voucher sequence comparisons and provides suggested identifications and sequence information in an easily interpreted spreadsheet format. This technique when applied to tuna and billfish larvae collected in the Straits of Florida had an 89% success rate. A single species (Thunnus atlanticus) was found to dominate the catch of tuna larvae, while billfish larvae were more evenly divided between two species (Makaira nigricans and Istiophorus platypterus). 相似文献
165.
ALAN D. CHRISTIAN EMY M. MONROE ALLISON M. ASHER JEANNETTE M. LOUTSCH DAVID J. BERG 《Molecular ecology resources》2007,7(4):570-573
Freshwater mussels (Unionidae) are among North America's most imperilled organisms. Mussels produce small larvae (glochidia) that parasitize aquatic vertebrates. We modified the Epicentre QuickExtract protocol to extract DNA from a single glochidium, collected directly from the marsupium of a female mussel, to use as template in polymerase chain reactions (PCRs). Yield per glochidium in a 40 µL extraction volume provided enough DNA for ≥ 15 PCRs per individual. We were successful in using this DNA for microsatellite analysis of up to three loci per individual. Offspring from one female showed evidence for multiple paternity within her brood. Our results are the first documentation of this phenomenon in freshwater mussels. 相似文献
166.
LAURA M. BOYKIN RUTH ANN BAGNALL DONALD R. FROHLICH DAVID G. HALL WAYNE B. HUNTER CATHERINE S. KATSAR CINDY L. MCKENZIE ROSEMARIE C. ROSELL ROBERT G. SHATTERS JR. 《Molecular ecology resources》2007,7(6):1202-1204
Twelve polymorphic microsatellite markers were developed from microsatellite‐enriched DNA libraries and mined from an expressed sequence tags library of Diaphorina citri, the vector of the citrus greening disease (huanglongbing). Analysis of 288 individuals from Florida, Texas, and Brazil showed that allelic diversity ranged from three to eight alleles per locus and observed and expected heterozygosities ranged from 0.014 to 0.569 and from 0.052 to 0.653, respectively. These variable microsatellite loci can provide means for assessing overall genetic variation and migration patterns for this agriculturally important pest species. This information can be used to aid in developing successful management strategies. 相似文献
167.
CLARE J. HOPKINS NOEL O. I. COGAN MELANIE HAND ERICA JEWELL JATINDER KAUR XI LI GERALDINE A. C. LIM ALISON E. LING CHRISTOPHER LOVE HAYLEY MOUNTFORD MARIJA TODOROVIC MEGAN VARDY GERMAN C. SPANGENBERG DAVID EDWARDS JACQUELINE BATLEY 《Molecular ecology resources》2007,7(4):697-700
The availability of expressed sequence data derived from gene discovery programs enables mining for simple sequence repeats (SSR), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development and characterization of 16 expressed sequence tags (EST)‐SSR markers from Brassica juncea and their cross‐amplification across Brassica species. Sixteen primer pairs were assessed for polymorphism in all genomes of the diploid and amphidiploid Brassica species. The markers show reliable amplification, considerable polymorphism and high transferability across species, demonstrating the utility of EST‐SSRs for genetic analysis of brassicas. 相似文献
168.
JACQUELINE BATLEY CLARE J. HOPKINS NOEL O. I. COGAN MELANIE HAND ERICA JEWELL JATINDER KAUR SUKHJIWAN KAUR XI LI ALISON E. LING CHRISTOPHER LOVE HAYLEY MOUNTFORD MARIJA TODOROVIC MEGAN VARDY MARZENA WALKIEWICZ GERMAN C. SPANGENBERG DAVID EDWARDS 《Molecular ecology resources》2007,7(5):886-889
The availability of expressed sequence data derived from gene discovery programs enables mining for simple sequence repeats (SSR), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development and characterization of 24 expressed sequence tags (EST)‐SSR markers from Brassica napus and their cross‐amplification across Brassica species. The markers show reliable amplification, genome specificity and considerable polymorphism, demonstrating the utility of EST‐SSRs for genetic analysis of wild Brassica populations and commercial Brassica germplasm. 相似文献
169.
HALVOR KNUTSEN DAVID FIANI HANNE SANNS A. RUS HOELZEL 《Molecular ecology resources》2007,7(5):851-853
We developed polymerase chain reaction primers for nine dinucleotide microsatellite loci in the marine deep‐sea fish, the tusk (Brosme brosme). All markers were obtained from a partial genomic DNA library, and characterized in 100 unrelated individuals from one putative population. The number of alleles ranged from two to 60 with an average of 15/locus. The observed heterozygosity ranged from 0.020 to 0.826 (average 0.438). Several of the markers amplified multiple alleles from the two other gadoid species tested. 相似文献
170.
Twenty members of the fly families Apioceridae and Mydidae are compared in terms of 77 adult characters. Cladistic analysis of 91 synapomorphies provides a well corroborated reconstruction of lineage relationships, and reveals that the Apioceridae is paraphyletic with respect to the Mydidae. In order to render the Apioceridae monophyletic, the genus Rhaphiomidas and the subfamily Megascelinae of the Apioceridae are transferred to the Mydidae. The subfamily Rhaphiomidinae is reinstated to accommodate Rhaphiomidas , comprising the most plesiomorphic mydids. The relationships of the remaining subfamilies of Mydidae are discussed in the context of these findings. The genus Apiocera is divided into four subgenera, the type subgenus Apiocera Westwood in Australia, Pyrocera subgen. nov. in North America, and the subgenera Ripidosyrma Hermann and Anypenus Philippi are applied to the South African and South American species, respectively. A key to the four subgenera of Apiocera is provided. The biogeographic relationships of the subgenera and genera of the Apioceridae and the Megascelinae are discussed. Although considered an example of a transantarctic or Gondwanan group, we argue that the distribution of the Apioceridae predates the Mesozoic supercontinent Gondwanaland and extends onto sections of Pangaea, and should be termed 'Pangaean'. The cladistic relationships between the genera of Apioceridae and Megascelinae are consistent with the geological vicariance of the fragments of Pangaea on which they now occur. 相似文献