Managing calcareous grassland for the declining Duke of Burgundy <Emphasis Type="Italic">Hamearis lucina</Emphasis> butterfly: effects of grazing management on <Emphasis Type="Italic">Primula</Emphasis> host plants

Many butterfly species are declining in range and abundance, sometimes to the point of becoming vulnerable to extinction. Several traits increase a species’ vulnerability to population decline through stochastic processes, including high larval specificity and poor dispersal rate. The Duke of Burgundy Hamearis lucina relies on Primula as its sole larval host plant. This monophagus dependency, coupled with susceptibility to environmental stochasticity, low dispersal and poor recolonization potential, means it is vital that sites supporting this rapidly-declining species are managed optimally. Here, we use two calcareous grassland sites in the UK with different grazing systems to identify optimal grazing management for Primula abundance and, for the first time, Primula characteristics linked previously to Duke oviposting preference and success: size, condition, succulence and surrounding sward height. We find that autumn and winter grazing intensity are both positively associated with Primula abundance, but there is a trade-off for winter grazing with negative effects on plant size, condition and succulence. Winter grazing also decreased the sward height below the optimum. Plants were bigger and better at the site managed using continuous (free-roaming) grazing versus the site managed using rotational (paddock-based) grazing. We recommend moderately high grazing intensity during autumn using a free-roaming system where possible to attain abundant Primula, with a reduction in grazing intensity or grazing removal during winter to ensure suitability of individual plants. This management would also benefit other host plants, such as bird’s foot trefoil Lotus corniculatus, which are vital for other declining butterflies that frequently co-occur with Dukes.     

Synergism of thymol,carvacrol and eugenol in larvae of the cattle tick,Rhipicephalus microplus,and brown dog tick,Rhipicephalus sanguineus

The effects of combinations of the monoterpenes thymol and carvacrol and the phenylpropanoid eugenol in larvae of Rhipicephalus microplus (Canestrini, 1888) (Acari: Ixodidae) and Rhipicephalus sanguineus sensu lato (s.l.) (Acari: Ixodidae) were assessed by the larval packet test. The CompuSyn program was used to make qualitative assessments of the effects (synergistic, additive and antagonistic) of the associations. The effects of all combinations tested against R. microplus larvae were synergistic, with combination indices (CIs) <0.70. When tested against R. sanguineus, eight of the mixtures showed a synergistic effect (CI < 0.70); only the carvacrol + thymol mixture at LC₅₀ presented a moderate synergistic effect, with CIs between 0.70–0.90. This study is the first to determine the effects of the interactions of these substances in the control of these two tick species. The combinations of carvacrol + thymol, carvacrol + eugenol and thymol + eugenol have synergistic effects in R. microplus and R. sanguineus s.l. larvae.     

Fertility table and rate of population growth of the predator Supputius cincticeps (Heteroptera: Pentatomidae) on one plant of Eucalyptus cloeziana in the field

The reproductive potential and population growth (r_m and λ) of the predator Supputius cincticeps (Heteroptera: Pentatomidae) were evaluated using a life and fertility table. S. cincticeps was reared on one plant of Eucalyptus cloeziana in the field and fed with Tenebrio molitor (Coleoptera: Tenebrionidae) pupae. Females of this predator had a net reproductive rate (R₀) of 21.02 females/female; an intrinsic rate of population increase (r_m) of 0.041 and finite rate of increase (λ) of 1.042. This resulted in population growth of S. cincticeps in the eucalyptus plant with a doubling time of 17.01 days. This natural enemy can be reared under field conditions with alternative prey for use in biological control. Such individuals of S. cinticeps will be better adapted to field conditions when they are liberated.     

Brood size: a major factor influencing male dimorphism in the non‐pollinating fig wasp Sycobia sp.

• 1 Male polymorphisms have been described in some non‐pollinating fig wasps, as well as in other animals. The proximal basis and the maintenance of alternative male reproductive strategies are either genetic or environmental.
• 2 Here we studied male dimorphism in the non‐pollinating fig wasp Sycobia sp. We conducted experimental manipulations to study the factors influencing offspring male morph allocations and explore a possible basis for the determination and maintenance of male dimorphism in Sycobia sp.
• 3 The results showed that brood size was the major and underlying factor influencing the male morph ratio. When the brood size increases, the wingless male ratio also increases.
• 4 Also, our results indicated that there was no direct maternal control on offspring male morph allocation.
• 5 Male dimorphism in Sycobia sp. probably represents an environmentally determined conditional strategy, which responded to offspring population density at the level of the individual fig.

     

Seed germination of Pilosocereus arrabidae (Cactaceae) from a semiarid region of south‐east Brazil

We evaluated the effect of temperature regimes (six constant and four alternating temperatures), light qualities (five red : far red ratios) and water potentials (ΨW; seven NaCl and polyethylene glycol 6000 [PEG] solutions) on the percentage and germination rate, as well as the post‐seminal development morphology, that allow Pilosocereus arrabidae seeds to germinate in a hot semiarid climate on the south‐eastern Brazilian coast. The results showed that seeds germinated similarly between constant and alternating temperatures, with an optimal germination at 25/20°C and 20°C. Pilosocereus arrabidae seeds were photoblastic positive and the final germination percentage was inhibited at low red : far red ratios. Maximum germination was obtained in distilled water (0 MPa) and decreases of Ψ_W in the solutions reduced the germination, which was lower in NaCl than in iso‐osmotic PEG solutions. Germination inhibition appears to be osmotic because the recovery response was high when non‐germinated seeds from both iso‐osmotic solutions were transferred to water. Seeds of P. arrabidae are small and germination is phaneroepigeal. Despite the slow growth typically seen in seedlings and adults of Cactaceae, germination in this species depends on the ability of the seeds to appropriately sense and react to environmental cues that correlate with times and places under low‐risk growth conditions.     

Acanthamoeba Interaction with Extracellular Matrix Glycoproteins: Biological and Biochemical Characterization and Role in Cytotoxicity and Invasiveness

ABSTRACT. Acanthamoeba are free-living amoebae that are dispersed in most environments. Occasionally, Acanthamoeba cause serious human infections, such as keratitis and encephalitis. During the infection process, amoebic adhesion to, and degradation of, host cells and their extracellular matrix (ECM) appear to be important requirements. We examined the interaction of Acanthamoeba with the ECM, and related this event to host cell destruction and tissue invasion. Pathogenic Acanthamoeba culbertsoni differentially attached on the ECM glycoproteins laminin-1, collagen-I, and fibronectin, as compared with non-pathogenic Acanthamoeba astronyxis . Binding to collagen-I and laminin-1 induced A. culbertsoni to become flattened and elongated. Because attachment on laminin-1 was higher in A. culbertsoni , laminin-1 was chosen for further analysis. A 55-kDa laminin-binding protein was identified in pathogenic amoebae, but it was not found in non-pathogenic amoebae. No differential cytotoxicity against distinct cell types was observed between A. culbertsoni incubated with or without ECM. On the other hand, binding on collagen-I or matrigel scaffolds induced a differential effect where A. culbertsoni invaded collagen-I matrices more rapidly. These results indicate that ECM recognition, as an antecedent to tissue invasion, may be a trait characteristic of pathogenic Acanthamoeba .