全文获取类型
收费全文 | 461篇 |
免费 | 40篇 |
国内免费 | 32篇 |
出版年
2022年 | 4篇 |
2020年 | 6篇 |
2019年 | 4篇 |
2018年 | 10篇 |
2017年 | 8篇 |
2016年 | 11篇 |
2015年 | 15篇 |
2014年 | 11篇 |
2013年 | 19篇 |
2012年 | 22篇 |
2011年 | 28篇 |
2010年 | 37篇 |
2009年 | 26篇 |
2008年 | 23篇 |
2007年 | 14篇 |
2006年 | 31篇 |
2005年 | 16篇 |
2004年 | 12篇 |
2003年 | 12篇 |
2002年 | 7篇 |
2001年 | 14篇 |
2000年 | 6篇 |
1999年 | 7篇 |
1998年 | 17篇 |
1997年 | 9篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 9篇 |
1992年 | 9篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 6篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1977年 | 5篇 |
1963年 | 3篇 |
1959年 | 3篇 |
1958年 | 4篇 |
1957年 | 4篇 |
1956年 | 4篇 |
1955年 | 8篇 |
1954年 | 4篇 |
1953年 | 7篇 |
1952年 | 9篇 |
1951年 | 12篇 |
1949年 | 5篇 |
排序方式: 共有533条查询结果,搜索用时 78 毫秒
1.
2.
3.
At-sea metabolism (CO2 production) and water turnover of six breeding Grey-headed Albatrosses Diomedea chrysostoma were measured, using the doubly labelled water method, at Bird Island, South Georgia, Mean food consumption (estimated from a water influx rate of 1.01 1 d-1 and data on dietary composition) was 1200gd-1 or 50.4 W. At-sea metabolism (derived from a rate of CO2 production of 3.98 1 h-1 ) was 27.7 W, 2.5 times the estimated basal metabolic rate (BMR). On average the birds ingested nearly twice as much food energy as they expended to obtain it. The metabolic rate during flight (estimated from at-sea metabolism and activity budget data) was 36.3 W (range 34.7–39.0 W) or 3.2 (range 3.0–3.4) times the predicted BMR. This is the lowest cost of flight yet measured, but consistent with the highly developed adaptations for economic flight shown by albatrosses. These results are briefly compared with data for other polar vertebrates (penguins, fur seals) exploiting similar prey. 相似文献
4.
Payant V; Abukashawa S; Sasseville M; Benkel BF; Hickey DA; David J 《Molecular biology and evolution》1988,5(5):560-567
Nuclear DNA was extracted from each of the eight species comprising the
Drosophila melanogaster species subgroup. Southern hybridization of this
DNA by using a molecular probe specific for the alpha-amylase coding region
showed that the duplicated structure of the amylase locus, first found in
D. melanogaster, is conserved among all species of the melanogaster
subgroup. Evidence is also presented for the concerted evolution of the
duplicated genes within each species. In addition, it is shown that the
glucose repression of amylase gene expression, which has been extensively
studied in D. melanogaster, is not confined to this species but occurs in
all eight members of the species subgroup. Thus, both the duplicated gene
structure and the glucose repression of Drosophila amylase gene activity
are stable over extended periods of evolutionary time.
相似文献
5.
JOÃO BATISTA TAVARES DA SILVA ISAAC ROITMAN 《The Journal of eukaryotic microbiology》1990,37(6):521-523
ABSTRACT. Three strains of Phytomonas serpens two from tomatoes, Lycopersicon esculentum one from the insect Phtia picta (Hemiptera, Coreidae), were cultivated in a chemically defined medium developed from a defined medium for cultivating insect flagellates. Besides organic growth factors required by other insect trypanosomatids this flagellate requires, serine and inositol. Glutamine stimulates growth, and, surprisingly, does not require heme. 相似文献
6.
Borrelia burgdorferi is a spirochete pathogen transmitted among warm-
blooded hosts by ixodid ticks. Frequency-dependent selection for variant
outer-surface proteins might be expected to arise in this species, since
rare variants are more likely to avoid immune surveillance in previously
infected hosts. We sequenced the OspA and OspB genes of nine North American
strains and compared them with nine strains previously described. For each
gene, the mean number of synonymous substitutions per synonymous site and
the mean number of nonsynonymous substitutions per nonsynonymous site show
only a twofold excess of silent mutations. Synonymous rates vary widely
along the OspB protein. Some regions show a significant excess of silent
substitutions, while divergence in other regions is constrained by biased
base composition or selection. The presence, in antigenically important
regions of the protein, of significant variation among strains, as well as
evidence for recombination among strains, should be considered in attempts
to develop vaccines against this disease.
相似文献
7.
M P ROBINSON G BUTCHER R H CURTIS K G DA VIES K EVANS 《The Annals of applied biology》1993,123(2):337-347
Two monoclonal antibodies, which differentially recognise the two species of potato cyst nematodes (PCN), Globodera pallida and G. rostochiensis, are described. They have been shown to have potential for quantification of these two species, recognising proteins of the same molecular weight (34 kD) in both species. Further investigation showed these proteins to have isoelectric points at pH values of 5.7 in G. pallida and 5.9 in G. rostochiensis, in common with the proteins used by Fleming & Marks (1983) to differentiate the species of PCN. They are likely to be structurally very similar, with the same physiological function (and therefore similar concentrations) in the two species. In cross-reactivity tests with a wide range of soil nematode species, the antibodies reacted strongly only with species of the genus Globodera, and thereby confirmed their potential as the basis of a quantitative immunoassay likely to be useful in management of PCN populations. 相似文献
8.
9.
10.