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241.
Potential efficacy of zileuton, a 5-LOX inhibitor, was evaluated for the reduction of pulmonary adenomas in the A/J murine
model when administered via nose-only inhalation. Development of pulmonary adenomas was induced with benzo(a)pyrene. Animals were treated with a zileuton solution (5 mg/mL in 85:15 ethanol/water) either twice weekly or five times
a week via nose-only inhalation; The placebo solution (85:15 EtOH/H2O, no active) was also evaluated. Dose delivered was calculated to be 1.2 mg/kg per exposure for each zileuton group. After
20 weeks of treatment, surface tumors were enumerated and histologically assessed. A significant reduction in tumor count
was noted for both the twice weekly administration (40%) and the five times a week administration (59%). The data also showed
a significant reduction for the group, which received the placebo (approximately 58%). The treatment groups were also found
to have an impact on the histological stages of adenoma development. 相似文献
242.
D C Flenley 《BMJ (Clinical research ed.)》1983,286(6369):955-959
243.
D H Doherty 《Journal of virology》1982,43(2):641-654
The T4 mutation ptg19-80 affects the mechanism of capsid-length determination. It is located in gene 23, which encodes the major structural protein of the capsid. The mutation results in the production of abnormal-length capsids in high frequencies. This paper describes the isolation and partial characterization of second-site revertants of ptg19-80. In the course of their analysis, it was discovered that ptg19-80 is itself a double mutation consisting of a gene 23 mutation (ptg19-80c), which causes the morphogenetic defect, and a suppressor mutation located near the lysozyme gene. Phenotypic characterization of nine pseudo-wild-type revertants of this double-mutation revealed that these revertants all produced lower frequencies of abnormal capsids than did ptg19-80. Seven of these revertants were shown to contain two suppressor mutations, one mapping in or near gene 22 and done mapping in or near gene 24. Both mutations were required for suppression. These suppressors displayed no discernible phenotype in the absence of ptg19-80c. 相似文献
244.
H. Levesque P. Delepelaire P. Rouzé J. Slightom D. Tepfer 《Plant molecular biology》1988,11(6):731-744
Analysis of published sequences for Ri TL-DNA (root-inducing left-hand transferred DNA) of Agrobacterium rhizogenes revealed several unsuspected structural features. First, Ri TL-DNA genes are redundant. Using redundancy as a criterion, three regions (left, middle and right) were discerned. The left one, ORFs (open reading frames) 1–7, contains no detectable redundancy. In the middle region a highly diverged gene family was detected in ORFs 8, 11, 12, 13 and 14. The right region contains an apparently recent duplication (ORF 15 =18+17). We interpret the phenomenon of redundancy, particularly in the central region that encodes the transformed phenotype, to be an adaptation that ensures function in a variety of host species. Comparison of Ri TL-DNA and Ti T-DNAs from Agrobacterium tumefaciens revealed common structures, unpredicted by previous nucleic acid hybridization studies. Ri TL-DNA ORF 8 is a diverged Ti T-DNA tms1. Both Agrobacterium genes consist of a member of the diverged gene family detected in the central part of the Ri TL-DNA, but fused to a sequence similar to iaaM of Pseudomonas savastonoi. Other members of this gene family were found scattered throughout Ti T-DNA. We argue that the central region of Ri and the part of Ti T-DNA including ORFs 5–10 evolved from a common ancestor. We present the hypothesis that the gene family encodes functions that alter developmental plasticity in higher plants. 相似文献
245.
I N Trakht I D Grozdova N N Guliaev E S Severin N V Gnuchev 《Biokhimii?a (Moscow, Russia)》1980,45(5):788-792
The true slime mould Physarum polycephalum was treated with various agents by spraying them upon the cell surface 4 hrs before the second synchronous mitosis. The onset of mitosis was considerably approximated after the plasmodium treatment with protein kinases from rat hepatoma or Ph. polycephalum at the late G2 phase. The catalytic and regulatory subunits of cAMP-dependent pig brain protein kinase caused retardation of mitosis, while the holoenzyme, casein kinase and alkaline phosphatase did not affect the timing of mitosis. The cyclic nucleotides and inhibitors of their metabolic enzymes were used to investigate the role of phosphorylation processes in the mitotic cycle. 相似文献
246.
Lucia Di Giambattista P. Grimaldi S. Gaudenzi D. Pozzi M. Grandi S. Morrone I. Silvestri A. Congiu Castellano 《European biophysics journal : EBJ》2010,39(6):929-934
We have made a preliminary analysis of the results about the effects on tumoral cell line (lymphoid T cell line Jurkat) induced
by UVB radiation (dose of 310 mJ/cm2) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy
(FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identification of the vibrational modes of some
of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique
has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction
of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations
between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et
al. in Radiat Res 168:698–705, 2007). In the present work, the results have shown significant changes in the absorbance and
spectral pattern in the wavenumber protein and nucleic acids regions after the treatments. 相似文献
247.
248.
M Traczewska D Chmielewska M Ochocka 《Folia haematologica (Leipzig, Germany : 1928)》1985,112(4):525-536
The in vivo effect of thymus factor X (TFX) on the E-rosetting capacity, the absolute peripheral blood lymphocytes and T-cell number per microliter, the skin test reactivity to recall antigens, and the immunoglobulin production was evaluated in 20 children with acute lymphoblastic leukaemia. The in vitro effect of TFX was also tested. The mean percentage of E-rosettes in these patients increased from 50 to 64%. Although absolute peripheral blood lymphocyte and T-cell number per microliter rose significantly, the mean values did not reach those in healthy children. The tests with recall antigens were positive in 13% of patients prior to immunotherapy and 32% following the therapy. The influence of immunotherapy on infectious episodes and on the stabilisation of remission was also evaluated. TFX in vivo appears to restore immunocompetence, decrease infections, and prolong remissions in children with ALL in remission. 相似文献
249.
Mandana Nikpour Dafna D Gladman Dominique Ibanez Paula J Harvey Murray B Urowitz 《Arthritis research & therapy》2010,12(3):R125
Introduction
Total cholesterol (TC) and blood pressure (BP) are likely to take a dynamic course over time in patients with systemic lupus erythematosus (SLE). This would have important implications in terms of using single-point-in-time measurements of these variables to assess coronary artery disease (CAD) risk. The objective of this study was to describe and quantify variability over time of TC and BP among patients with SLE and to determine their correlates. 相似文献250.