全文获取类型
收费全文 | 842009篇 |
免费 | 104369篇 |
国内免费 | 362篇 |
专业分类
946740篇 |
出版年
2016年 | 9276篇 |
2015年 | 13152篇 |
2014年 | 15468篇 |
2013年 | 22200篇 |
2012年 | 24537篇 |
2011年 | 25073篇 |
2010年 | 16653篇 |
2009年 | 15619篇 |
2008年 | 22209篇 |
2007年 | 23107篇 |
2006年 | 21459篇 |
2005年 | 20965篇 |
2004年 | 20492篇 |
2003年 | 20109篇 |
2002年 | 19324篇 |
2001年 | 36304篇 |
2000年 | 36860篇 |
1999年 | 29475篇 |
1998年 | 10805篇 |
1997年 | 11517篇 |
1996年 | 10981篇 |
1995年 | 10458篇 |
1994年 | 10382篇 |
1993年 | 10350篇 |
1992年 | 25557篇 |
1991年 | 25246篇 |
1990年 | 24460篇 |
1989年 | 24021篇 |
1988年 | 22217篇 |
1987年 | 21489篇 |
1986年 | 20181篇 |
1985年 | 20307篇 |
1984年 | 17055篇 |
1983年 | 14974篇 |
1982年 | 11645篇 |
1981年 | 10658篇 |
1980年 | 10126篇 |
1979年 | 16825篇 |
1978年 | 13219篇 |
1977年 | 12181篇 |
1976年 | 11541篇 |
1975年 | 12729篇 |
1974年 | 13225篇 |
1973年 | 13063篇 |
1972年 | 12151篇 |
1971年 | 10810篇 |
1970年 | 9345篇 |
1969年 | 8884篇 |
1968年 | 8084篇 |
1967年 | 7104篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
901.
The complete amino acid sequence of histone H3 (135 residues) from the nematode Caenorhabditis elegans has been established. Microheterogeneity occurs at positions 96 and 100 of the chain. The sequences of the nematode H3 isoforms are very similar to the major chain of calf thymus H3 with which they show 4 substitutions in total. The major variant has cysteine in position 96. This is the first report of cysteine in this position in H3 from non-mammalian tissue. An exceptional methylation site has been detected at position 79. Various other sites of secondary modification are of a conservative nature. 相似文献
902.
The effect of selection for methidathion resistance on fitness components of aP. persimilis strain was analysed by different means. The resistant strain was compared with the susceptible one from which it was selected. The life history and some parameters essential to the successful use of this species in biological control (voracity, resistance to starvation and drought) were analysed. Methidathion resistance was followed for 6 months under rearing conditions free of insecticide in the resistant and in a mixed strain. The investigations showed that the fitness of resistant mites did not seem altered by methidathion selection. It thus appears possible to increase pesticide resistance in beneficial arthropods without adversely affecting their main fitness components. 相似文献
903.
904.
905.
906.
907.
908.
R Druga 《Folia morphologica》1989,37(1):57-63
The existence of ipsilateral claustrocortical projection to the occipital area Oc 2.2 (Wree et al. 1981) was demonstrated in four guinea pigs. The neurons labelled by retrogradely transported HRP were localized in the caudal half of the claustrum dorsale (in its dorsomedial and central part); small and medium-sized oval and multipolar neurons preponderated. 相似文献
909.
In an effort to facilitate studies of the reaction involved in the removal of fatty acids from acyl proteins, we have synthesized an octanoic acid ester of doubly blocked serine, specifically octanoyl N-carbobenzoxy-L-serine-benzyl ester (octanoyl boc-serine), and used it as a substrate to guide the purification of an esterase from rat lung. The esterase was purified 228-fold by column chromatography on DE-52 cellulose, hydroxylapatite, octyl-Sepharose, and concanavalin A-Sepharose and by HPLC gel filtration. The final enzyme preparation ran as a single 77,000-Da band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and exhibited a single symmetrical peak (sedimentation coefficient, 4.5 S) when centrifuged through a sucrose density gradient (empirical Mr, 63,000). The esterase is an acidic protein, pI 4.1, and is very active against p-nitrophenyl esters comprised of C4-C14 fatty acids; the highest specific activity (26.5 mumol/min/mg) was obtained using p-nitrophenyl caprylate as substrate. The pH optimum of the lung esterase is near 8.0 and the activity on octanoyl boc-serine is maximum when 0.3% (w/v) Myrj-52 is included in the assay medium. The activity of the esterase is not dependent on calcium ions. The enzyme does not remove acyl groups from the G-protein of vesicular stomatitis virus or the proteolipid of bovine brain. The possible role of the esterase in the metabolism of acylated proteins is considered. 相似文献
910.