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971.
The use of a mixture model in the analysis of count data 总被引:1,自引:0,他引:1
A mixture model is presented for the analysis of data on premature ventricular contractions. The analysis is shown to be straightforward and the conclusions relatively simple. 相似文献
972.
V. Cencora F. Challoner S. Gooch A. Livingstone D. Widgery 《BMJ (Clinical research ed.)》1988,297(6646):481-483
973.
Structural analysis of human profilin has revealed two tryptophan residues, W3 and W31, which interact with polyproline. The codons for these residues were mutated to encode phenylalanine and the mutant proteins overexpressed in Eschericia coli. The isolated proteins were diminished in their ability to bind polyproline, whereas phosphatidylinositol 4,5-bisphosphate (PIP2) binding remained unchanged. In many strains of Saccharomyces cerevisiae, disruption of the gene encoding profilin, PFY1, is lethal. It was found that expression of the gene for human profilin is capable of suppressing this lethality. The polyproline-binding mutant alleles of the human gene were cloned into various yeast expression vectors. Each of the mutant genes resulted in suppression of the lethality of pfy1Delta. It was observed that the mutant protein expression levels paralleled the growth rates of the strains. The severity of various morphological abnormalities of the strains was also attenuated with increased protein levels, suggesting that profilin polyproline-binding mutations are deleterious to cell growth unless overexpressed. Both tryptophan mutations were combined to give a third mutant allele that was found both unable to bind polyproline and to suppress the lethality of a pfy1 deletion. Immunoprecipitation experiments suggested that the mutants were unaltered in their affinity for actin and PIP2. These data strongly suggest that polyproline binding is an essential function of profilin. 相似文献
974.
975.
976.
A tracer method is described that uses the stable isotope 30Si to measure rates of silicic acid uptake by diatom cultures and natural populations of marine phytoplankton. The method involves (i) incubation of organisms requiring silicic acid for growth in the presence of 30Si-labeled silicic acid, (ii) collection of the resulting particulate silicon, (iii) conversion of the particulate silicon to BaSiF6, (iv) determination of the 30Si content of BaSiF6 by solid sample mass spectrometry, and (v) calculation of the uptake rate from the 30Si enrichment of the particulate matter during the incubation. The maximum overall error in the uptake rate measurement is ±10%. 相似文献
977.
The uptake of L-glutamate into BHK21-C13 cells in culture has been studied. This amino acid appears to be transported via a relatively high affinity, low capacity, Na+-dependent transport system capable of the rapid accumulation of substrate amino acids. Kinetic studies of the inhibition of L-glutamate uptake has provided information as to the substrate and the molecular configuration required for transport via the glutamate transport system. This system exhibited marked substrate specificity and was only capable of transporting L-glutamate and aspartate and certain closely related acidic amino acid analogues. 相似文献
978.
K. D. Elsey 《Journal of nematology》1977,9(4):338-342
Howardula dominicki n. sp. is described from specimens collected from the tobacco flea beetle, Epitrix hirtipennis (Melsheimer), at Oxford, North Carolina , and is distinguished from other members of the genus . Parasitism by H. dominicki sterilized female flea beetles and often led to the death of larvae. 相似文献
979.
980.