全文获取类型
收费全文 | 103790篇 |
免费 | 7667篇 |
国内免费 | 382篇 |
专业分类
111839篇 |
出版年
2014年 | 52篇 |
2013年 | 51篇 |
2012年 | 12382篇 |
2011年 | 13765篇 |
2010年 | 2090篇 |
2009年 | 971篇 |
2008年 | 10268篇 |
2007年 | 10549篇 |
2006年 | 9690篇 |
2005年 | 9088篇 |
2004年 | 8495篇 |
2003年 | 7802篇 |
2002年 | 6616篇 |
2001年 | 5141篇 |
2000年 | 6586篇 |
1999年 | 2633篇 |
1998年 | 335篇 |
1997年 | 213篇 |
1996年 | 160篇 |
1995年 | 163篇 |
1994年 | 150篇 |
1993年 | 170篇 |
1992年 | 140篇 |
1991年 | 123篇 |
1990年 | 85篇 |
1989年 | 110篇 |
1988年 | 113篇 |
1987年 | 93篇 |
1986年 | 86篇 |
1985年 | 75篇 |
1984年 | 79篇 |
1983年 | 78篇 |
1982年 | 64篇 |
1981年 | 53篇 |
1974年 | 38篇 |
1972年 | 42篇 |
1971年 | 36篇 |
1970年 | 44篇 |
1959年 | 135篇 |
1958年 | 315篇 |
1957年 | 270篇 |
1956年 | 221篇 |
1955年 | 247篇 |
1954年 | 260篇 |
1953年 | 222篇 |
1952年 | 186篇 |
1951年 | 209篇 |
1950年 | 180篇 |
1949年 | 80篇 |
1948年 | 80篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
93.
94.
An arginase, purified from the leaf of the jack bean, Canavalia ensiformis, can effectively hydrolyze both l- and d-arginine. Arginases, examined from a number of other plant and animal sources, exhibit marked substrate stereospecificity and fail to catabolize d-arginine. In order to provide essential nitrogen, jack bean leaf arginase also catabolizes l-canavanine, an arginine analog that is a predominant nitrogen-storing metabolite of this legume. The ability of arginase to metabolize both stereoisomers of arginine may result from the requirement for this enzyme to exhibit limited substrate specificity in order to hydrolyze both arginine and canavanine. 相似文献
95.
Gadkari D Schricker K Acker G Kroppenstedt RM Meyer O 《Applied and environmental microbiology》1990,56(12):3727-3734
The novel thermophilic CO- and H(2)-oxidizing bacterium UBT1 has been isolated from the covering soil of a burning charcoal pile. The isolate is gram positive and obligately chemolithoautotrophic and has been named Streptomyces thermoautotrophicus on the basis of G+C content (70.6 +/- 0.19 mol%), a phospholipid pattern of type II, MK-9(H(4)) as the major quinone, and other chemotaxonomic and morphological properties. S. thermoautotrophicus could grow with CO (t(d) = 8 h), H(2) plus CO(2) (t(d) = 6 h), car exhaust, or gas produced by the incomplete combustion of wood. Complex media or heterotrophic substrates such as sugars, organic acids, amino acids, and alcohols did not support growth. Molybdenum was required for CO-autotrophic growth. For growth with H(2), nickel was not necessary. The optimum growth temperature was 65 degrees C; no growth was observed below 40 degrees C. However, CO-grown cells were able to oxidize CO at temperatures of 10 to 70 degrees C. Temperature profiles of burning charcoal piles revealed that, up to a depth of about 10 to 25 cm, the entire covering soil provides a suitable habitat for S. thermoautotrophicus. The K(m) was 88 mul of CO liter and V(max) was 20.2 mul of CO h mg of protein. The threshold value of S. thermoautotrophicus of 0.2 mul of CO liter was similar to those of various soils. The specific CO-oxidizing activity in extracts with phenazinemethosulfate plus 2,6-dichlorophenolindophenol as electron acceptors was 246 mumol min mg of protein. In exception to other carboxydotrophic bacteria, S. thermoautotrophicus CO dehydrogenase was able to reduce low potential electron acceptors such as methyl and benzyl viologens. 相似文献
96.
Kaiden D 《The Einstein quarterly journal of biology and medicine》1996,12(4):134-141
"This paper will discuss the following: why population policies and programs have traditionally focused on women; what the limitations of current male contraceptives are and why research into new methods [has] proved unfruitful; and why these policies and programs would be more productive if they were to direct more attention toward men." 相似文献
97.
Living yeast cells can be selectively stained with the lipophilic cationic cyanine dye DiOC6(3) in a mitochondrial membrane potential-dependent manner. Our study extends the use of flow cytometric analysis and sorting to DiOC6(3)-stained yeast cells. Experimental conditions were developed that prevented the toxic side effect of the probe and gave a quantitative correlation between fluorescence and mitochondrial membrane potential, without any staining of other membranes. The localization of the fluorochrome was checked by confocal microscopy and image cytometry. The mitochondrial membrane alterations were also tested through cardiolipin staining with nonyl acridine orange. Differences in light scattering and in fluorescence were detected in mutants (rho-, rho degrees, mit-, or pet-) and wild-type (rho+mit+) populations of yeast. The dye uptake of respiratory-deficient yeast strains was significantly reduced as compared to that of the wild-type. Application of an uncoupler (mCICCP), which collapsed the mitochondrial membrane potential (alphapsi(m)), led to a drastic reduction of the dye uptake. It was observed that a decrease in deltapsi(m), was usually correlated with a decrease in cardiolipin stainability by nonyl acridine orange (NAO). Quantitative flow cytometry is a fast and reproducible technique for rapid screening of yeast strains that might be suspected of respiratory dysfunction and/or mitochondrial structural changes. We give evidence that it is an adequate method to characterize and isolate respiratory mutants through sorting procedure, with selective enrichment of the population studied in respiring or non-respiring yeast cells. Confocal microscopy and image cytometry corroborate the flow cytometry results. 相似文献
98.
99.
Root growth and cell differentiation were analysed in lentil seedlings grown (1) in microgravity (F microg), (2) on the 1 x g centrifuge (F1 x g), (3) in microgravity and placed on the 1 x g centrifuge for 4 h [F(microg + 1 x g)], (4) on the 1 x g centrifuge and placed in microgravity for 4 h [F(1 x g + microg)]. In microgravity, there were strong oscillations of the root tip, even when the seedlings were grown first on the 1 x g centrifuge [F(1 x g + microg)]. In the [F(microg + 1 x g)] sample, the roots grown in microgravity were oblique with respect to the 1 x g acceleration when the seedlings were placed on the centrifuge. They were therefore gravistimulated. However, root length was similar in the 4 samples after 29 h of growth and growth rate of the root was the same between 25 h and 29 h although it appeared to be slightly greater in the [F(microg + 1 x g)] sample. Cell elongation was analysed as a function of the distance from the root cap junction. Cell length was similar in the seedlings grown in microgravity or on the 1 x g centrifuge. The transfer from the 1 x g centrifuge to microgravity [F(1 x g + microg)] did not modify cell elongation in the roots. Cell length in the roots which were grown in microgravity and gravistimulated [F(microg + 1 x g)] was different from that observed in microgravity but this was only due to gravistimulation. Thus, gravity does not have an effect on cell elongation when the roots are strictly oriented in the vertical position but it does as soon as the root tip deviates from this orientation. 相似文献
100.
Na+-K+ Exchange at the Xylem/Symplast Boundary (Its Significance in the Salt Sensitivity of Soybean) 总被引:2,自引:0,他引:2 下载免费PDF全文
We investigated the mechanism of Na+ reabsorption in exchange for K+ at the xylem/symplast boundary of soybean roots (Glycine max var Hodgson). The xylem vessels of excised roots were perfused with solutions of defined composition to discriminate between entry of ions into or reabsorption from the xylem vessels. In the presence of NaCl, the transport systems released K+ into the xylem sap and reabsorbed Na+. The Na+-K+ exchange was energized by proton-translocating ATPases, enhanced by external K+ concentration, and dependent on the anion permeability. Evidence was presented for the operation of H+/Na+ and H+/K+ antiporters at the xylem/symplast interface. 相似文献