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41.
Evolution of the Adh locus in the Drosophila willistoni group: the loss of an intron, and shift in codon usage 总被引:1,自引:0,他引:1
We report here the DNA sequence of the alcohol dehydrogenase gene (Adh)
cloned from Drosophila willistoni. The three major findings are as follows:
(1) Relative to all other Adh genes known from Drosophila, D. willistoni
Adh has the last intron precisely deleted; PCR directly from total genomic
DNA indicates that the deletion exists in all members of the willistoni
group but not in any other group, including the closely related saltans
group. Otherwise the structure and predicted protein are very similar to
those of other species. (2) There is a significant shift in codon usage,
especially compared with that in D. melanogaster Adh. The most striking
shift is from C to U in the wobble position (both third and first
position). Unlike the codon-usage-bias pattern typical of highly biased
genes in D. melanogaster, including Adh, D. willistoni has nearly 50% G + C
in the third position. (3) The phylogenetic information provided by this
new sequence is in agreement with almost all other molecular and
morphological data, in placing the obscura group closer to the melanogaster
group, with the willistoni group farther distant but still clearly within
the subgenus Sophophora.
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Dott. CL. CH. Mathon 《Plant biosystems》2013,147(2-3):345-356
Resumé Chez les Blés branchus de l'espèce «turgidum» (Triticum turgidum compositum), il existe des sortes à thermostade plutôt froid (Blés d'hiver ou de semi-hiver) et des sortes à thermostade chaud, tièe ou «indifférent» (Blés de printemps). Les Blés branchus de l'espèce «turgidum» apparaissent comme étant des plantes à photostade de jour long. La plus ou moins grande rapidité de l'accomplissement du photostade, par rapport à la rapidité de l'assimilation des matières plastiques, détermine la structure, — non ramifiée ou ramifiée — de l'épi. 相似文献
44.
SGO1 but not SGO2 is required for maintenance of centromere cohesion in Arabidopsis thaliana meiosis
L. Zamariola N. De Storme CL. Tiang S. J. Armstrong F. C. H. Franklin D. Geelen 《Sexual plant reproduction》2013,26(3):197-208
Shugoshin is a protein conserved in eukaryotes and protects sister chromatid cohesion at centromeres in meiosis. In our study, we identified the homologs of SGO1 and SGO2 in Arabidopsis thaliana. We show that AtSGO1 is necessary for the maintenance of centromere cohesion in meiosis I since atsgo1 mutants display premature separation of sister chromatids starting from anaphase I. Furthermore, we show that the localization of the specific centromeric cohesin AtSYN1 is not affected in atsgo1, suggesting that SGO1 centromere cohesion maintenance is not mediated by protection of SYN1 from cleavage. Finally, we show that AtSGO2 is dispensable for both meiotic and mitotic cell progression in Arabidopsis. 相似文献
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Survey of Malassezia sp and dermatophytes in the cutaneous microbiome of free‐ranging golden‐headed lion tamarins (Leontopithecus chrysomelas ‐ Kuhl, 1820)
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AAM Coelho-Castelo AP Trombone RS Rosada RR Santos Jr VLD Bonato A Sartori CL Silva 《Genetic vaccines and therapy》2006,4(1):1-10
In order to assess a new strategy of DNA vaccine for a more complete understanding of its action in immune response, it is important to determine the in vivo biodistribution fate and antigen expression. In previous studies, our group focused on the prophylactic and therapeutic use of a plasmid DNA encoding the Mycobacterium leprae 65-kDa heat shock protein (Hsp65) and achieved an efficient immune response induction as well as protection against virulent M. tuberculosis challenge. In the present study, we examined in vivo tissue distribution of naked DNA-Hsp65 vaccine, the Hsp65 message, genome integration and methylation status of plasmid DNA. The DNA-Hsp65 was detectable in several tissue types, indicating that DNA-Hsp65 disseminates widely throughout the body. The biodistribution was dose-dependent. In contrast, RT-PCR detected the Hsp65 message for at least 15 days in muscle or liver tissue from immunized mice. We also analyzed the methylation status and integration of the injected plasmid DNA into the host cellular genome. The bacterial methylation pattern persisted for at least 6 months, indicating that the plasmid DNA-Hsp65 does not replicate in mammalian tissue, and Southern blot analysis showed that plasmid DNA was not integrated. These results have important implications for the use of DNA-Hsp65 vaccine in a clinical setting and open new perspectives for DNA vaccines and new considerations about the inoculation site and delivery system. 相似文献