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81.
Witold Gadkowski Magorzata Grabarczyk Katarzyna Wiska Barbara Ratu Agata Biaoska Zbigniew Ciunik Czesaw Wawrzeczyk 《Journal of Molecular Catalysis .B, Enzymatic》2007,49(1-4):79-87
The strain Absidia cylindrospora was chosen among eight fungal strains for the biotransformation of unsaturated lactones 1a–c. The processes were carried out by means of shaken cultures. The compounds 1a and 1b were efficiently converted into the corresponding trans-epoxylactones (2a and 2b), whereas the transformation of 1c gave the unsaturated hydroxylactone 3, with the tertiary hydroxy group introduced in the allylic position. The compound 2b was obtained with 100% ee. The structures of compounds 2a and 2b were fully confirmed by the X-ray analysis, which showed the half boat and half chair conformation of cyclohexane ring in these molecules, respectively. All the products were not reported previously in the literature. 相似文献
82.
Darzynkiewicz ZM Bojarska E Stepinski J Jemielity J Jankowska-Anyszka M Davis RE Darzynkiewicz E 《Nucleosides, nucleotides & nucleic acids》2007,26(10-12):1349-1352
Eukaryotic cells utilize scavenger decapping enzymes to degrade cap structure following 3'-5' mRNA decay. Human DcpS recently has been described as a highly specific hydrolase (a member of the HIT family) that catalyses the cleavage of m(7)GpppG and short capped oligoribonucleotides. We have demonstrated here that cap-1 (m(7)GpppGm) is a preferred substrate among several investigated dinucleotide cap analogues m(7)Gp(n)N (n = 3-5, N is a purine or pyrimidine base) and m(7)GMP is always one of the reaction product. Cap analogues containing pyrimidine base instead of guanine or diphosphate chain are resistant to hydrolysis catalyzed by human scavenger. Contrary to the other enzymes of HIT family, hDcpS activity is not stimulated by Mg(2+). 相似文献
83.
A reversible decrease in ribulose 1,5‐bisphosphate carboxylase/oxygenase carboxylation activity caused by the aggregation of the enzyme's large subunit is triggered in response to the exposure of moderate irradiance‐grown plants to low irradiance
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Magda Grabsztunowicz Zbigniew Górski Robert Luciński Grzegorz Jackowski 《Physiologia plantarum》2015,154(4):591-608
Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) is highly regulated in response to fluctuations in the environment, including changes in irradiance. However, no complex data are available on Rubisco regulatory mechanisms triggered in plants which are submitted to moderate–low irradiance shift. Therefore, we investigated in a comprehensive way the changes at the level of amount of Rubisco protein, its structural organization and carboxylase activity of the holoenzyme as triggered by exposure of moderate irradiance‐grown Arabidopsis thaliana plants to low irradiance conditions. An exposure of moderate irradiance‐grown plants to low irradiance for a single photoperiod caused the exclusion of a certain pool of Rubisco under altered conditions owing to oxidative modifications resulting in the formation of protein aggregates involving Rubisco large subunit (LS). As a result, both initial and total Rubisco carboxylase activities were reduced, whereas Rubisco activation state remained largely unchanged. The results of the determination of reactive oxygen species indicated that a moderate/low irradiance transition had stimulated 1O2 accumulation and we strongly suggest that Rubisco oxidative modifications leading to formation of aggregates encompassing Rubisco‐LS were triggered by 1O2. When moderate irradiance regime was resumed, the majority of Rubisco‐LS containing aggregates tended to be resolubilized, and this allowed Rubisco carboxylation activities to be largely recovered, without changes in the activation state of the enzyme. In the longer term, these results allow us to better understand a complexity of Rubisco regulatory mechanisms activated in response to abiotic stresses and during recovery from the stresses. 相似文献
84.
85.
N‐terminal guanidinylation of the cyclic 1,4‐ureido‐deltorphin analogues: the synthesis,receptor binding studies,and resistance to proteolytic digestion
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Krzysztof Bańkowski Olga M. Michalak Anna Leśniak Katarzyna E. Filip Piotr Cmoch Zbigniew Szewczuk Piotr Stefanowicz Jan Izdebski 《Journal of peptide science》2015,21(6):467-475
The synthesis of a series of N‐guanidinylated cyclic ureidopeptides, analogues of 1,4‐ureido‐deltorphin/dermorphine tetrapeptide is described. The δ‐ and μ‐opioid receptor affinity of new guanidinylated analogues and their non‐guanidinylated precursors was determined by the displacement radioligand binding experiments. Our results indicate that the guanidinylation of cyclic 1,4‐ureidodeltorphin peptide analogues does not exhibit a uniform influence on the opioid receptor binding properties, similarly as reported earlier for some linear peptides. All analogues were also tested for their in vitro resistance to proteolysis during incubation with large excess of chymotrypsin, pepsin, and papain by means of mass spectroscopy. Guanidinylated ureidopeptides 1G–4G showed mixed μ agonist/δ agonist properties and high enzymatic stability indicating their potential as therapeutic agents for treatment of pain. Copyright © 2015 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
86.
Lekka M Gil D Dąbroś W Jaczewska J Kulik AJ Lekki J Stachura Z Stachura J Laidler P 《Journal of molecular recognition : JMR》2011,24(5):833-842
The expression of N‐cadherin, characteristic of various cancers, very often leads to changes in the cells' adhesive properties. Thus, we sought to find out if N‐cadherin expressed in various, but cancer‐related cells, differs in its functional properties that could contribute to variations in cells' phenotypes. In our work, measurements of an unbinding force of a single N‐cadherin molecule, probed with the same antibody both on a surface of living non‐malignant (HCV29) and malignant cells (T24) of bladder cancer, were carried out with the use of an atomic force microscopy. The results show the enhanced N‐cadherin level in T24 malignant cells (8.7% vs. 3.6% obtained for non‐malignant one), confirmed by the Western blot and the immunohistochemical staining. The effect was accompanied by changes in unbinding properties of an individual N‐cadherin molecule. Lower unbinding force values (26.1 ± 7.1 pN) in non‐malignant cells reveal less stable N‐cadherin complexes, as compared to malignant cells (61.7 ± 14.6 pN). This suggests the cancer‐related changes in a structure of the binding site of the antibody, located at the extracellular domain of N‐cadherin. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
87.
Katarzyna Kapczyńska Piotr Stefanowicz Łukasz Jaremko Mariusz Jaremko Alicja Kluczyk Zbigniew Szewczuk 《Amino acids》2011,40(3):923-932
Protein glycation is often a cause of diabetes-associated complications. The isotopically labeled peptide-derived Amadori
products may serve as standards for quantitative determination of the glycated proteins. In this paper, we discussed various
approaches to the synthesis of Amadori products labeled selectively with stable isotopes 2H, 13C and 18O. 相似文献
88.
Liyan Hu Xin Liu Yana Chervona Feikun Yang Moon-shong Tang Zbigniew Darzynkiewicz Wei Dai 《Cell cycle (Georgetown, Tex.)》2011,10(14):2373-2379
Disruption of cell cycle checkpoints and interference with the normal cell cycle progression frequently result in cell death or malignant transformation. Hexavalent chromium [Cr(VI)] is a well-known carcinogen that has been implicated in the occurrence of many types of human malignancies, including lung cancer. However, the exact mechanism by which Cr(VI) causes malignant transformation in the lung remains unknown. We have demonstrated that chronic exposure to a noncytotoxic concentration of Cr(VI) induced a variety of chromosomal abnormalities, including premature sister chromatid separation, chromosomal breakage and the presence of lagging/misaligned chromosomes. After treatment with nocodazole, both HeLa and normal lung bronchial epithelial cells were arrested at mitosis. However, Cr(VI) significantly compromised M-phase arrest induced by nocodazole. Cr(VI) suppressed BubR1 activation and reduced expression of Emi1, leading to an unscheduled activation of APC/C. Consistent with this observation, Cr(VI) treatment caused enhanced polyubiquitination of geminin during mitotic release, while it deregulated the activity of Cdt1, a DNA replication licensing factor. Combined, these results suggest that Cr(VI)-induced chromosomal instability is partly due to a perturbation of APC/C activities, leading to chromosomal instability.Key words: chromium, checkpoint, chromosome instability, APC/C, BubR1, Emi1 相似文献
89.
Bilayer lipid membranes composed of phosphatidylcholine and decanoic acid or phosphatidylcholine and decylamine were investigated
using electrochemical impedance spectroscopy. Interaction between membrane components causes significant deviations from the
additivity rule. Area, capacitance, and stability constant values for the complexes were calculated based on the model assuming
1:1 stoichiometry, and the model was validated by comparison of these values to experimental results. We established that
phosphatidylcholine and decylamine form highly stable 1:1 complexes. In the case of decanoic acid-modified phosphatidylcholine
membranes, complexes with stoichiometries other than 1:1 should be taken into consideration. 相似文献
90.
Nineteen compounds isolated from field samples of cyanobacteria Woronichinia naegeliana (Unger) Elenkin were identified. They fell into four classes of peptides: cyanopeptolins (cyanopeptolin B, cyanopeptolin C, cyanopeptolin D, cyanopeptolin 880, micropeptin 88D, micropeptin 478‐B, micropeptin SD999, micropeptin T2, planktopeptin BL1061), microginins (microginin 478, microginin 757, microginin 51A, microginin 91E, microginin FR3, microginin FR4), anabaenopeptins (oscillamide B) and possibly microcystins (trace amounts of microcystin‐LR) showing respectively fragment patterns in their electrospray ion source‐MS spectra. The molecular masses of the determined peptides range from 700 to 1100 Da. These results confirm the remarkable ability of cyanobacteria to synthesize a wide array of peptides. 相似文献