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991.
Archie EA Maldonado JE Hollister-Smith JA Poole JH Moss CJ Fleischer RC Alberts SC 《Molecular ecology》2008,17(11):2666-2679
Nonrandom patterns of mating and dispersal create fine-scale genetic structure in natural populations — especially of social mammals — with important evolutionary and conservation genetic consequences. Such structure is well-characterized for typical mammalian societies; that is, societies where social group composition is stable, dispersal is male-biased, and males form permanent breeding associations in just one or a few social groups over the course of their lives. However, genetic structure is not well understood for social mammals that differ from this pattern, including elephants. In elephant societies, social groups fission and fuse, and males never form permanent breeding associations with female groups. Here, we combine 33 years of behavioural observations with genetic information for 545 African elephants ( Loxodonta africana ), to investigate how mating and dispersal behaviours structure genetic variation between social groups and across age classes. We found that, like most social mammals, female matrilocality in elephants creates co-ancestry within core social groups and significant genetic differentiation between groups (ΦST = 0.058). However, unlike typical social mammals, male elephants do not bias reproduction towards a limited subset of social groups, and instead breed randomly across the population. As a result, reproductively dominant males mediate gene flow between core groups, which creates cohorts of similar-aged paternal relatives across the population. Because poaching tends to eliminate the oldest elephants from populations, illegal hunting and poaching are likely to erode fine-scale genetic structure. We discuss our results and their evolutionary and conservation genetic implications in the context of other social mammals. 相似文献
992.
Egídio Torrado Jeffrey J. Fountain Richard T. Robinson Cynthia A. Martino John E. Pearl Javier Rangel-Moreno Michael Tighe Robert Dunn Andrea M. Cooper 《PloS one》2013,8(4)
Cell-mediated immune responses are known to be critical for control of mycobacterial infections whereas the role of B cells and humoral immunity is unclear. B cells can modulate immune responses by secretion of immunoglobulin, production of cytokines and antigen-presentation. To define the impact of B cells in the absence of secreted immunoglobulin, we analyzed the progression of Mycobacterium tuberculosis (Mtb) infection in mice that have B cells but which lack secretory immunoglobulin (AID−/−µS−/−mice). AID−/−µS−/− mice accumulated a population of activated B cells in the lungs when infected and were more susceptible to aerosol Mtb when compared to wild type (C57BL/6) mice or indeed mice that totally lack B cells. The enhanced susceptibility of AID−/−µS−/− mice was not associated with defective T cell activation or expression of a type 1 immune response. While delivery of normal serum to AID−/−µS−/− mice did not reverse susceptibility, susceptibility in the spleen was dependent upon the presence of B cells and susceptibility in the lungs of AID−/−µS−/−mice was associated with elevated expression of the cytokines IL-6, GM-CSF, IL-10 and molecules made by alternatively activated macrophages. Blocking of IL-10 signaling resulted in reversal of susceptibility in the spleens and lungs of AID−/−µS−/− mice. These data support the hypothesis that B cells can modulate immunity to Mtb in an organ specific manner via the modulation of cytokine production and macrophage activation. 相似文献
993.
Human responses are critical to understanding injury biomechanics in blunt ballistic impacts, which are defined as 20-200 g projectiles impacting at 20-250 m/s. 13 human cadavers were exposed to three distinct ballistic impacts of the chest to determine force-time, deflection-time and force-deflection responses. Comparisons were made between biomechanical responses for ballistic impacts and those previously reported for lower speed, higher mass impacts. Impact condition B (140 g at 40 m/s) gave the largest peak force 10,602+/-2226 N and deflection 54.7+/-14.6 mm. Impact condition A (140 g at 20 m/s) involved lower impact energy and produced lower peak force 3383+/-761 N and deflection 25.9+/-3.1 mm, as did impact condition C (40 g at 60 m/s), which gave 3158+/-309 N and 20.1+/-7.8 mm. The results indicate each impact condition gives distinctive responses, which differ from those previously reported in the automotive literature for lower speed impacts. This information provides the foundation for future biomechanical research in the area of blunt ballistic impacts, specifically the development of test surrogates and evaluation of protective equipment. 相似文献
994.
von Jan M Lapidus A Del Rio TG Copeland A Tice H Cheng JF Lucas S Chen F Nolan M Goodwin L Han C Pitluck S Liolios K Ivanova N Mavromatis K Ovchinnikova G Chertkov O Pati A Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Saunders E Brettin T Detter JC Chain P Eichinger K Huber H Spring S Rohde M Göker M Wirth R Woyke T Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP 《Standards in genomic sciences》2010,2(3):327-346
Archaeoglobus profundus (Burggraf et al. 1990) is a hyperthermophilic archaeon in the euryarchaeal class Archaeoglobi, which is currently represented by the single family Archaeoglobaceae, containing six validly named species and two strains ascribed to the genus 'Geoglobus' which is taxonomically challenged as the corresponding type species has no validly published name. All members were isolated from marine hydrothermal habitats and are obligate anaerobes. Here we describe the features of the organism, together with the complete genome sequence and annotation. This is the second completed genome sequence of a member of the class Archaeoglobi. The 1,563,423 bp genome with its 1,858 protein-coding and 52 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
995.
Susan H. Williams Christopher J. Vinyard Kenneth E. Glander Max Deffenbaugh Mark F. Teaford Cynthia L. Thompson 《International journal of primatology》2008,29(6):1441-1453
In vivo laboratory-based studies describing jaw-muscle activity and mandibular bone strain during mastication provide the empirical
basis for most evolutionary hypotheses linking primate masticatory apparatus form to diet. However, the laboratory data pose
a potential problem for testing predictions of these hypotheses because estimates of masticatory function and performance
recorded in the laboratory may lack the appropriate ecological context for understanding adaptation and evolution. For example,
in laboratory studies researchers elicit rhythmic chewing using foods that may differ significantly from the diets of wild
primates. Because the textural and mechanical properties of foods influence jaw-muscle activity and the resulting strains,
chewing behaviors studied in the laboratory may not adequately reflect chewing behaviors of primates feeding in their natural
habitats. To circumvent this limitation of laboratory-based studies of primate mastication, we developed a system for recording
jaw-muscle electromyograms (EMGs) from free-ranging primates so that researchers can conduct studies of primate jaw-muscle
function in vivo in the field. We used the system to record jaw-muscle EMGs from mantled howlers (Alouatta palliata) at Hacienda La Pacifica, Costa Rica. These are the first EMGs recorded from a noncaptive primate feeding in its natural
habitat. Further refinements of the system will allow long-term EMG data collection so that researchers can correlate jaw-muscle
function with food mechanical properties and behavioral observations. In addition to furthering understanding of primate feeding
biology, our work will foster improved adaptive hypotheses explaining the evolution of primate jaw form. 相似文献
996.
Mary A. Robinson Stephen W. Tuttle Cynthia M. Otto Cameron J. Koch 《Free radical biology & medicine》2010,48(2):189-195
Stimulated macrophages produce nitric oxide (NO) via inducible nitric oxide synthase (iNOS) using molecular O2, L-arginine, and NADPH. Exposure of macrophages to hypoxia decreases NO production within seconds, suggesting substrate limitation as the mechanism. Conflicting data exist regarding the effect of pO2 on NADPH production via the oxidative pentose phosphate cycle (OPPC). Therefore, the present studies were developed to determine whether NADPH could be limiting for NO production under hypoxia. Production of NO metabolites (NOx) and OPPC activity by RAW 264.7 cells was significantly increased by stimulation with lipopolysaccharide (LPS) and interferon γ (IFNγ) at pO2 ranging from 0.07 to 50%. OPPC activity correlated linearly with NOx production at pO2 > 0.13%. Increased OPPC activity by stimulated RAW 264.7 cells was significantly reduced by 1400 W, an iNOS inhibitor. OPPC activity was significantly increased by concomitant treatment of stimulated RAW 264.7 cells with chemical oxidants such as hydroxyethyldisulfide or pimonidazole, at 0.07 and 50% O2, without decreasing NOx production. These results are the first to investigate the effect of pO2 on the relationship between NO production and OPPC activity, and to rule out limitations in OPPC activity as a mechanism by which NO production is decreased under hypoxia. 相似文献
997.
Melnick J Lis E Park JH Kinsland C Mori H Baba T Perkins J Schyns G Vassieva O Osterman A Begley TP 《Journal of bacteriology》2004,186(11):3660-3662
The genes encoding thiamine kinase in Escherichia coli (ycfN) and thiamine pyrophosphokinase in Bacillus subtilis (yloS) have been identified. This study completes the identification of the thiamine salvage enzymes in bacteria. 相似文献
998.
Beigneux AP Kosinski C Gavino B Horton JD Skarnes WC Young SG 《The Journal of biological chemistry》2004,279(10):9557-9564
ATP-citrate lyase (Acly) is one of two cytosolic enzymes that synthesize acetyl-coenzyme A (CoA). Because acetyl-CoA is an essential building block for cholesterol and triglycerides, Acly has been considered a therapeutic target for hyperlipidemias and obesity. To define the phenotype of Acly-deficient mice, we created Acly knockout mice in which a beta-galactosidase marker is expressed from Acly regulatory sequences. We also sought to define the cell type-specific expression patterns of Acly to further elucidate the in vivo roles of the enzyme. Homozygous Acly knockout mice died early in development. Heterozygous mice were healthy, fertile, and normolipidemic on both chow and high fat diets, despite expressing half-normal amounts of Acly mRNA and protein. Fibroblasts and hepatocytes from heterozygous Acly mice contained half-normal amounts of Acly mRNA and protein, but this did not perturb triglyceride and cholesterol synthesis or the expression of lipid biosynthetic genes regulated by sterol regulatory element-binding proteins. The expression of acetyl-CoA synthetase 1, another cytosolic enzyme for producing acetyl-CoA, was not up-regulated. As judged by beta-galactosidase staining, Acly was expressed ubiquitously but was expressed particularly highly in tissues with high levels of lipogenesis, such as in the livers of mice fed a high-carbohydrate diet. beta-Galactosidase staining was intense in the developing brain, in keeping with the high levels of de novo lipogenesis of the tissue. In the adult brain, beta-galactosidase staining was in general much lower, consistent with reduced levels of lipogenesis; however, beta-galactosidase expression remained very high in cholinergic neurons, likely reflecting the importance of Acly in generating acetyl-CoA for acetylcholine synthesis. The Acly knockout allele is useful for identifying cell types with a high demand for acetyl-CoA synthesis. 相似文献
999.
Phosphoramidates are modified deoxyoligonucleotides that feature nitrogen in place of the 3'-oxygen of a phosphodiester linkage. Noted for stability against nuclease activity, these linkages are of both mechanistic and therapeutic interest. While a number of studies characterizing the properties of oligonucleotides composed entirely of phosphoramidate linkages have been published, little is known about how singly substituted phosphoramidate substitutions affect the thermodynamics and structure of protein-oligonucleotide interactions. We chose to investigate these interactions with PvuII endonuclease, the DNA binding behavior of which is well-characterized. Oligonucleotide duplexes containing a phosphoramidate substitution at the scissile phosphates were resistant to cleavage by the enzyme, even after extended incubations. However, the enzyme was able to cleave the native strand in a native:phosphoramidate heteroduplex at a rate comparable to that observed with the native substrate. Ca(II)-stimulated PvuII binding for a phosphoramidate-substituted oligonucleotide is comparable to that of the native duplex (K(d) approximately 200 pM). K(d) values obtained in the presence of Mg(II) are somewhat weaker (K(d) approximately 10 nM). Under metal-free conditions, the enzyme exhibited a remarkable approximately 50-fold greater affinity for the modified oligonucleotide relative to the native substrate (5 vs 240 nM). While (31)P NMR spectra indicate increased chemical shift dispersion in the free phosphoramidate duplex, the spectrum of the enzyme-bound duplex is similar to that of the native duplex. (1)H-(15)N HSQC analysis indicates that enzyme conformations in the presence of these oligonucleotides are also comparable. The tight binding of the phosphoramidate duplex under metal-free conditions and its resistance to cleavage are attributed to local conformational adjustments propagating from the O-->N substitution. 相似文献
1000.