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This is a correction article 相似文献
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RGEN STENVIK METTE SERINE WESMAJERVI KJERSTI TURID FJALESTAD B
RGE DAMSGRD MADJID DELGHANDI 《Molecular ecology resources》2006,6(4):1105-1107
Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety‐two novel microsatellite loci (tetra‐, tri‐ and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene‐associated polymorphic microsatellite markers (11 tri‐ and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02–0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers. 相似文献
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Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater 总被引:1,自引:0,他引:1
Cusick RD Bryan B Parker DS Merrill MD Mehanna M Kiely PD Liu G Logan BE 《Applied microbiology and biotechnology》2011,89(6):2053-2063
A pilot-scale (1,000 L) continuous flow microbial electrolysis cell was constructed and tested for current generation and
COD removal with winery wastewater. The reactor contained 144 electrode pairs in 24 modules. Enrichment of an exoelectrogenic
biofilm required ~60 days, which is longer than typically needed for laboratory reactors. Current generation was enhanced
by ensuring adequate organic volatile fatty acid content (VFA/SCOD ≥ 0.5) and by raising the wastewater temperature (31 ± 1°C).
Once enriched, SCOD removal (62 ± 20%) was consistent at a hydraulic retention time of 1 day (applied voltage of 0.9 V). Current
generation reached a maximum of 7.4 A/m3 by the planned end of the test (after 100 days). Gas production reached a maximum of 0.19 ± 0.04 L/L/day, although most of
the product gas was converted to methane (86 ± 6%). In order to increase hydrogen recovery in future tests, better methods
will be needed to isolate hydrogen gas produced at the cathode. These results show that inoculation and enrichment procedures
are critical to the initial success of larger-scale systems. Acetate amendments, warmer temperatures, and pH control during
startup were found to be critical for proper enrichment of exoelectrogenic biofilms and improved reactor performance. 相似文献
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Debrah A. Fine Orit Rozenblatt-Rosen Megha Padi Anna Korkhin Robert L. James Guillaume Adelmant Rosa Yoon Luxuan Guo Christian Berrios Ying Zhang Michael A. Calderwood Soundarapandian Velmurgan Jingwei Cheng Jarrod A. Marto David E. Hill Michael E. Cusick Marc Vidal Laurence Florens Michael P. Washburn Larisa Litovchick James A. DeCaprio 《PLoS pathogens》2012,8(10)
The small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins for efficient viral replication. The SV40 large T antigen (LT) contains several discrete functional domains including the LXCXE or RB-binding motif, the DNA binding and helicase domains that contribute to the viral life cycle. In addition, the LT C-terminal region contains the host range and adenovirus helper functions required for lytic infection in certain restrictive cell types. To understand how LT affects the host cell to facilitate viral replication, we expressed full-length or functional domains of LT in cells, identified interacting host proteins and carried out expression profiling. LT perturbed the expression of p53 target genes and subsets of cell-cycle dependent genes regulated by the DREAM and the B-Myb-MuvB complexes. Affinity purification of LT followed by mass spectrometry revealed a specific interaction between the LT C-terminal region and FAM111A, a previously uncharacterized protein. Depletion of FAM111A recapitulated the effects of heterologous expression of the LT C-terminal region, including increased viral gene expression and lytic infection of SV40 host range mutants and adenovirus replication in restrictive cells. FAM111A functions as a host range restriction factor that is specifically targeted by SV40 LT. 相似文献
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Palmer ND Fort S Starkey IR Shaw TR Northridge DB 《International journal of cardiovascular interventions》2000,3(3):161-165
BACKGROUND: The technique of coronary stenting has evolved over recent years, with improved stent technology and effective antiplatelet therapies to prevent stent thrombosis. In Europe, reductions in stent and equipment costs have resulted from increased market competition. The impact of these changes on the in-hospital procedural cost of percutaneous coronary intervention (PCI) in the current clinical setting is not known. METHODS: We compared the initial equipment and pharmaceutical costs of one hundred consecutive, unselected patients undergoing PCI in 1998 to a similar population who underwent PCI in 1994. RESULTS: Similar patient characteristics were noted, yet more complex disease (multivessel, AHA type B2/C lesions) was treated in the 1998 population. The stent utilization rate (83% vs 15%, p < 0.0001) and use of intravenous and/or oral antiplatelet therapy (abciximab, ticlopidine) (64% vs 4%, p < 0.0001) was higher in 1998. Similar angiographic success was achieved in each group with low complication rates. Mean hospital stay was reduced in the 1998 group (2.6 +/- 2.8 vs 4.3 +/- 3.8 days, p < 0.001). Repeat PCI was required more frequently in the 1994 population (26% vs 9%, p < 0.001). Overall there was no significant difference in the mean equipment cost between the two groups ( pound 1551 vs pound 1422, p=ns). CONCLUSION: Despite the widespread use of coronary stenting and antiplatelet therapies there appears to be no difference in current in-hospital equipment costs for PCI compared to 1994. Improved clinical outcomes in the 1998 population imply that stenting is a cost-effective therapy. 相似文献
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HANNE Salmenkari ANITA LAITINEN RICHARD A. FORSGÅRD MERVI HOLAPPA JERE LINDÉN LAURI PASANEN MATTI KORHONEN RIITTA KORPELA JOHANNA NYSTEDT 《Cytotherapy》2019,21(2):175-188