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A sterile germination study with seeds of some common phanerogamic water plants showed almost 100 per cent germination for seeds of Alisma plantago–aquatica, Baldellia ranunculoides and Nymphaea alba. Seeds of Potamogeton lucens could be germinated to about 40 per cent, seeds of Polygonum amphibium germinated sporadically while those of Cladium mariscus could not be germinated at all. Freshly harvested seeds of Alisma and Baldellia showed an ability to germinate at both 20°C and 35°C. A stratification period of one month at +4°C gave germination of all species tested, with the exception of Cladium. Potamogeton germinated in light only, the other species both in light and darkness. Treatment times for surface sterilization in disinfectants are given. 相似文献
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Measurement of Serotonin Turnover Rate in Rat Dorsal Raphe Nucleus by In Vivo Electrochemistry 总被引:1,自引:1,他引:0
5-Hydroxytryptamine (5-HT; serotonin) turnover rate in dorsal raphe nucleus of the urethane-anesthetized rat was estimated by using the in vivo electrochemical detector to measure the decay of extraneuronal 5-hydroxyindole acetic acid (5-HIAA) after monoamine oxidase inhibition. Carbon paste electrodes were scanned by semiderivative voltammetry and revealed two peaks: one at +0.15 V and the other at +0.25 V. The higher potential peak is composed primarily of the 5-HT metabolite 5-HIAA. After administration of pargyline, 75 mg/kg i.p., this peak declined exponentially. Regression analysis of these data by an exponential decay model yielded the fractional rate constant 0.82 +/- 0.06 h-1 (mean +/- SEM). This rate constant of 5-HIAA disappearance measured by in vivo electrochemistry is identical to the rate constant found by others measuring 5-HIAA disappearance by direct tissue assay methods. In animals not treated with pargyline, tissue 5-HIAA concentrations in the dorsal raphe nucleus were measured by HPLC with electrochemical detection. The average 5-HT turnover rate calculated as the product of the fractional rate constant and steady-state tissue 5-HIAA concentration was 12.6 nmol/g/h. These results demonstrate that electrochemical detection of extraneuronal 5-HIAA combined with monoamine oxidase inhibition can be used to measure neurotransmitter turnover in vivo in a discrete brain region. 相似文献
126.
Curt D. Busse 《International journal of primatology》1984,5(3):247-261
A method is presented for measuring the location of individuals with respect to the center or perimeter of a primate group.
The method estimates an individual’s domain of danger: the area in which a hidden predator is closer to the individual than
to other group members (cf. Hamilton, 1971). A domain is determined by the directions and distances of particular neighbors
from a given individual. Animals at peripheral locations have relatively large domains, whereas animals at central locations
have relatively small domains. Domains of danger were sampled for members of two groups of chacma baboons (Papio ursinus)in northern Botswana, to test for nonrandom spatial patterns throughout the day. Females with infants had significantly smaller
domains than did females without infants. Domains also were correlated with social ranks of females;high-ranking females had smaller domains than did low-ranking females, possibly because high-ranking females were more likely
to have infant offspring. For adult males, however,domain sizes were not significantly correlated with social ranks. Immigration status of adult males, rather than social rank,
better accounted for spatial positioning. 相似文献
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During a study of chacma baboons, Papio ursinus, in the Moremi Wildlife Reserve, Botswana, the mothers of 10 infants disappeared. Pre-reproductive males and females, all 4 to 5 years old, adopted nine natural orphans, and one older orphan was not adopted. Compared with other troop members, 4- and 5-year-old caregivers would incur the lowest costs of adoption in terms of interference with their direct reproductive potential. In the five instances in which relatedness of natural orphans could be assessed, caregivers were probably close relatives of orphans they cared for. By comparison, 5-year-old males immediately adopted two experimentally introduced orphans. 相似文献
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