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41.
Chapelle Frédérique Steiman Regine Seigle-Murandi Françoise Luu-Duc Cuong 《Applied microbiology and biotechnology》1986,23(6):430-433
Summary The ability of 610 strains of micromycetes to introduce a hydroxy-group into benzimidazole substrate has been investigated.
Among them, 232 micromycetes have been selected and cultivated in a liquid synthetic medium. Convenient hydroxylation of benzimidazole
was obtained with 78 fungi. Finally, 8 strains of micromycetes have been chosen for further investigation and optimization
of this bioconversion. 相似文献
42.
An unusual m-hydroxyacetophenone, named viscidone, and three new chroman-4-one derivatives were isolated from Chrysothamnus viscidiflorus ssp. lanceolatus, which also contained a known p-hydroxyacetophenone derivative. Their structures were determined by spectroscopic (1H and 13C NMR, UV, IR and MS) and chemical means. 相似文献
43.
44.
5-hydroxylation of benzimidazole by micromycetes II. Optimisation of production with Absidia spinosa
Françoise Seigle-Murandi Régine Steiman Frédérique Chapelle Cuong Luu Duc 《Applied microbiology and biotechnology》1986,25(1):8-13
Summary The bioconversion of benzimidazole to 5-hydroxybenzimidazole was investigated as a function of the physiological state of the fungus and culture conditions. Using an inoculum composed only of spores, the addition of benzimidazole at different times of development led to a better definition of optimal physiological conditions for obtaining a good hydroxylation yield. The relationship between the degree of hydroxylation and that of culture medium aeration, was shown by the use of four different conditions: flowing medium on an inert support, in static, agitated or highly aerated medium. 相似文献
45.
The Vietnam Initiative on Zoonotic Infections (VIZIONS): A Strategic Approach to Studying Emerging Zoonotic Infectious Diseases 总被引:1,自引:0,他引:1
Maia A. Rabaa Ngo Tri Tue Tran My Phuc Juan Carrique-Mas Karen Saylors Matthew Cotten Juliet E. Bryant Ho Dang Trung Nghia Nguyen Van Cuong Hong Anh Pham Alessandra Berto Voong Vinh Phat Tran Thi Ngoc Dung Long Hoang Bao Ngo Thi Hoa Heiman Wertheim Behzad Nadjm Corina Monagin H. Rogier van Doorn Motiur Rahman My Phan Vu Tra James I. Campbell Maciej F. Boni Pham Thi Thanh Tam Lia van der Hoek Peter Simmonds Andrew Rambaut Tran Khanh Toan Nguyen Van Vinh Chau Tran Tinh Hien Nathan Wolfe Jeremy J. Farrar Guy Thwaites Paul Kellam Mark E. J. Woolhouse Stephen Baker 《EcoHealth》2015,12(4):726-735
46.
Cao C Kim JP Kim BW Chae H Yoon HC Yang SS Sim SJ 《Biosensors & bioelectronics》2006,21(11):2106-2113
A biochip based on surface plasmon resonance was fabricated to detect prostate specific antigen-alpha(1)-antichymotrypsin (PSA-ACT complex) in both HBS buffer and human serum. To reduce non-specific binding and steric hindrance effect, the chemical surface of the sensor chips was constructed by using various oligo(ethylene glycol) mixtures of different molar ratios of HS(CH2)11(OCH2CH2)6OCH2COOH and HS(CH2)11(OCH2CH2)3OH. The self-assembled monolayers were biotinylated to facilitate the immobilization of streptavidin. Using the chip surfaces, PSA-ACT complex in HBS buffer and human serum was detected at 20.7 and 47.5 ng/ml by primary immunoresponse, respectively. However, the limit of detection could be simply enhanced by a sandwich strategy to improve the sensitivity and specificity of the immunoassay. An intact PSA polyclonal antibody was used as an amplifying agent in the strategy. As a result, PSA-ACT complex concentrations as low as 10.2 and 18.1 ng/ml were found in the HBS buffer and human serum sample, respectively. The result indicates that this approach could satisfy our goal without modifying the secondary interactant. 相似文献
47.
Nestbox provision is a technique used to increase nest-site availability for secondary cavity-nesting birds. However, little is known about the demographic consequences of nestbox provision in different habitat types. To assess how nestbox provision affects the density of hole-nesting birds simultaneously in two contrasting habitats, we compared the breeding density of Great Tits along transects without nestboxes with that in transects where nestboxes were provided. Although the initial density of breeders was considerably higher in the deciduous habitat than in the coniferous habitat, provision of nestboxes increased density by a similar number of additional pairs in each habitat type. Thus, the provision of nestboxes in managed coniferous forests may be as effective in increasing the breeding opportunities of cavity nesters as in deciduous stands. Moreover, previous research showed that pairs in deciduous habitat with nestboxes have consistently lower breeding success than those in coniferous habitat with nestboxes. It is possible that the addition of nestboxes in the preferred habitat increased density to such an extent that density-dependent effects became apparent. 相似文献
48.
Pham Thanh Ky Pham Thanh Huong Than Kieu My Pham Tuan Anh Phan Van Kiem Chau Van Minh Nguyen Xuan Cuong Nguyen Phuong Thao Nguyen Xuan Nhiem Jae-Hee Hyun Hee-Kyoung Kang Young Ho Kim 《Phytochemistry》2010,71(8-9):994-1001
Dammarane-type saponins, gypenosides VN1–VN7 (1–7), were isolated from the total saponin extract of Gynostemma pentaphyllum aerial parts, with their structures elucidated on the basis of spectroscopic and chemical methods. These compounds showed moderate cytotoxic activity against four human cancer cell lines, A549 (lung), HT-29 (colon), MCF-7 (breast), and SK-OV-3 (ovary), with IC50 values ranging from 19.6 ± 1.1 to 43.1 ± 1.0 μM. Regarding the HL-60 (acute promyelocytic leukemia) cell line, compounds 1, 5, and 6 showed weakly active with IC50 values of 62.8 ± 1.9, 72.6 ± 3.6, and 82.4 ± 3.2 nM, respectively, while 2, 3, 4, and 7 were less active with IC50 values >100 μM. 相似文献
49.
Thao NT Hung TM Cuong TD Kim JC Kim EH Jin SE Na M Lee YM Kim YH Choi JS Min BS 《Bioorganic & medicinal chemistry letters》2010,20(24):7435-7439
Four new 28-nor-oleanane-type triterpene oligoglycosides, camellenodiol 3-O-β-D-galactopyranosyl(1→2)[β-D-xylopyranosyl(1→2)-β-D-galactopyranosyl(1→3)]-β-D-glucuronopyranoside (2), camellenodiol 3-O-4'-O-acetyl-β-D-galactopyranosyl(1→2)[β-D-xylopyranosyl(1→2)-β-D-galactopyranosyl(1→3)]-β-D-glucuronopyranoside (4), camellenodiol 3-O-(β-D-galactopyranosyl(1→2)[β-D-xylopyranosyl(1→2)-β-D-galactopyranosyl(1→3)]-6'-methoxy-β-D- glucuronopyranoside (5), and maragenin II 3-O-(β-D-galactopyranosyl(1→2)[β-D-xylopyranosyl(1→2)-β-D-galactopyranosyl(1→3)]-6'-methoxy-β-D-glucuronopyranoside (6), along with two known compounds, (1 and 3), were isolated from the stem bark of Camellia japonica. Their chemical structures were established mainly by 2D NMR techniques and mass spectrometry. The isolated compounds showed inhibitory effects on NO production in RAW264.7 macrophages. 相似文献
50.
Nguyen Xuan Cuong Nguyen Xuan Nhiem Nguyen Phuong Thao Nguyen Hoai Nam Nguyen Tien Dat Hoang Le Tuan Anh Le Mai Huong Phan Van Kiem Chau Van Minh Ji-Hee Won Won-Yoon Chung Young Ho Kim 《Bioorganic & medicinal chemistry letters》2010,20(16):4782-4784
Ten phenolic compounds (1–10) were isolated from a methanol extract of Lawsonia inermis leaves including two new ones, lawsoniasides A (1) and B (2). Their structures were elucidated by spectroscopic methods (NMR and FTICRMS) in combination with acid hydrolysis and GC analyses. Compounds 4 and 5 showed a significant inhibition on receptor activator for nuclear factor-κB ligand-induced osteoclast formation in murine bone-marrow macrophages. 相似文献