Ergothioneine attenuates varicocele-induced testicular damage by upregulating HSP90AA1 in rats

This study investigates the therapeutic effect and the underlying mechanisms of ergothioneine (EGT) on the testicular damage caused by varicocele (VC) in vivo, in vitro, and in silico. This preclinical study combines a series of biological experiments and network pharmacology analyses. A total of 18 Sprague Dawley (SD) male rats were randomly and averagely divided into three groups: the sham-operated, VC model, and VC model with EGT treatment (VC + EGT) groups. The left renal vein of the VC model and the VC + EGT groups were half-ligated for 4 weeks. Meanwhile, the VC + EGT group was intragastrically administrated with EGT (10 mg/kg). GC1 and GC2 cells were exposed to H₂O₂ with or without EGT treatment to re-verify the conclusion. The structure disorder of seminiferous tubules ameliorated the apoptosis decrease in the VC rats receiving EGT. EGT can also increase the sperm quality of the VC model rats (p < 0.05). The exposure to H₂O₂ decreased proliferation and increased apoptosis of GC1 and GC2 cells, which was revisable by adding EGT to the plates (p < 0.05). The network pharmacology and molecular docking were conducted to explore the potential targets of EGT in VC, and HSP90AA1 was identified as the pivotal gene, which was validated by western blot, immunohistochemistry, and RT-qPCR both in vivo and in vitro (p < 0.05). Overall, EGT attenuates the testicular injury in the VC model both in vivo and in vitro by potentially potentiating the expression of HSP90AA1.     

闲置鱼塘演变为雨水花园的景观再造——第十二届中国(南宁)国际园林博览会“芦草叠塘”景区设计

以南宁园博园“芦草叠塘”景区设计为例,探讨如何利用场地中闲置的坑塘景观,将其改造为与园博会功能相适应且具有未来弹性发展空间的景观,为类似项目的开发设计提供参考与借鉴。以低影响的生态理念,消纳雨水、过滤江水并融入人的活动,将水的表现形式作为主线贯穿景观设计始终,营造具有艺术氛围的雨水花园。景区成为园博盛会最受欢迎的景区之一,设计很好地平衡了会时展览与会后发展的关系,为打造生态可持续园博会提出新思路。