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91.
The high molecular weight microtubule-associated proteins MAP 1 and MAP 2 are major components of brain cytosol and can be readily identified using polyacrylamide gel electrophoresis on the basis of heat-stability and co-sedimentation with microtubules. An examination of synaptosomal cytosol, synaptic plasma membrane and postsynaptic density fractions showed that MAP 2 is absent from these fractions and thus from both pre- and postsynaptic sites. All of the fractions contained polypeptides that comigrated with MAP 1 and a MAP 1 like polypeptide was identified in a microtubule preparation from synaptosomal cytosol. The absence of MAP 2 from synaptosomal cytosol was confirmed by immunoblotting using an antibody directed against MAP 2. Immunocytochemistry using this antibody showed that MAP 2 was present in cell bodies and dendrites but absent from axons. 相似文献
92.
A set of surface proteins common to the circulating human platelet and lymphocyte (Short Communication) 总被引:1,自引:0,他引:1
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M. J. A. Tanner D. H. Boxer Jane Cumming J. Verrier-Jones 《The Biochemical journal》1974,141(3):909-911
The surface proteins of the circulating human platelet and lymphocyte were labelled by using the lactoperoxidase iodination method. Polyacrylamide-gel electrophoresis showed that four corresponding labelled proteins are found on the surface of each cell type. The most intensely labelled protein contains little or no carbohydrate, but the remaining labelled proteins are all glycoproteins. The major labelled band from each cell was isolated and comparative peptide ;maps' showed that the two proteins are closely similar. The surface proteins of the lymphocyte and platelet are distinct from those on the erythrocyte, the remaining major type of circulating cell. 相似文献
93.
Models of the human bronchial tree 总被引:18,自引:0,他引:18
94.
Selective substrates and inhibitors have been used to measure kinases phosphorylating endogenous proteins in rat liver nuclei during growth and regeneration after partial hepatectomy. Peaks in activity were found at 5, 22, and 29 hours after partial hepatectomy. Administration of 1 and adrenergic blockers suggested that the Be2+ sensitive and cyclic AMP-dependent protein kinases were interdependently regulated by Ca2+ and cyclic AMP. 相似文献
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Gordon R. Cumming 《CMAJ》1963,88(16):827-832
Angiotensin II was given to over 200 patients during heart catheterization studies. Left to right shunts through atrial and ventricular septal defects were increased. Shunts not detected under control conditions could then be recognized. In normal subjects left atrial pressure increased about 5-10 mm. Hg, producing a secondary rise in pulmonary artery pressure. The response of the pulmonary vessels to angiotensin was usually passive. Cardiac output declined in the normal subject after angiotensin administration. Right to left shunts decreased after angiotensin administration in some patients with cyanotic congenital heart disease, thus decreasing cyanosis. This finding has possible therapeutic applications. The pulmonary arterioles of patients with pulmonary hypertension may respond to angiotensin in a different manner from those with normal pulmonary arterioles. 相似文献
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