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941.
942.
Wilton P. Cruz Renato A. Sarmento Adenir V. Teodoro Marçal P. Neto Maíra Ignacio 《Experimental & applied acarology》2013,60(4):509-519
Seasonal changes in climate and plant diversity are known to affect the population dynamics of both pests and natural enemies within agroecosystems. In Brazil, spontaneous plants are usually tolerated in small-scale physic nut plantations over the year, which in turn may mediate interactions between pests and natural enemies within this agroecosystem. Here, we aimed to access the influence of seasonal variation of abiotic (temperature, relative humidity and rainfall) and biotic (diversity of spontaneous plants, overall richness and density of mites) factors on the communities of phytophagous and predatory mites found in a physic nut plantation and its associated spontaneous plants. Mite sampling was monthly conducted in dicotyledonous and monocotyledonous leaves of spontaneous plants as well as in physic nut shrubs over an entire year. In the dry season there was a higher abundance of phytophagous mites (Tenuipalpidae, Tarsonemidae and Tetranychidae) on spontaneous plants than on physic nut shrubs, while predatory mites (Phytoseiidae) showed the opposite pattern. The overall density of mites on spontaneous plants increased with relative humidity and diversity of spontaneous plants. Rainfall was the variable that most influenced the density of mites inhabiting physic nut shrubs. Agroecosystems comprising spontaneous plants associated with crops harbour a rich mite community including species of different trophic levels which potentially benefit natural pest control due to increased diversity and abundance of natural enemies. 相似文献
943.
David Cruz‐Garcia Maria Ortega‐Bellido Margherita Scarpa Julien Villeneuve Marko Jovic Marc Porzner Tamas Balla Thomas Seufferlein Vivek Malhotra 《The EMBO journal》2013,32(12):1717-1729
The BAR (Bin/Amphiphysin/Rvs) domain proteins arfaptin1 and arfaptin2 are localized to the trans‐Golgi network (TGN) and, by virtue of their ability to sense and/or generate membrane curvature, could play an important role in the biogenesis of transport carriers. We report that arfaptins contain an amphipathic helix (AH) preceding the BAR domain, which is essential for their binding to phosphatidylinositol 4‐phosphate (PI(4)P)‐containing liposomes and the TGN of mammalian cells. The binding of arfaptin1, but not arfaptin2, to PI(4)P is regulated by protein kinase D (PKD) mediated phosphorylation at Ser100 within the AH. We also found that only arfaptin1 is required for the PKD‐dependent trafficking of chromogranin A by the regulated secretory pathway. Altogether, these findings reveal the importance of PI(4)P and PKD in the recruitment of arfaptins at the TGN and their requirement in the events leading to the biogenesis of secretory storage granules. 相似文献
944.
Chikwendu Ibebunjo Joel M. Chick Tracee Kendall John K. Eash Christine Li Yunyu Zhang Chad Vickers Zhidan Wu Brian A. Clarke Jun Shi Joseph Cruz Brigitte Fournier Sophie Brachat Sabine Gutzwiller QiCheng Ma Judit Markovits Michelle Broome Michelle Steinkrauss Elizabeth Skuba Jean-Rene Galarneau Steven P. Gygi David J. Glass 《Molecular and cellular biology》2013,33(2):194-212
Molecular mechanisms underlying sarcopenia, the age-related loss of skeletal muscle mass and function, remain unclear. To identify molecular changes that correlated best with sarcopenia and might contribute to its pathogenesis, we determined global gene expression profiles in muscles of rats aged 6, 12, 18, 21, 24, and 27 months. These rats exhibit sarcopenia beginning at 21 months. Correlation of the gene expression versus muscle mass or age changes, and functional annotation analysis identified gene signatures of sarcopenia distinct from gene signatures of aging. Specifically, mitochondrial energy metabolism (e.g., tricarboxylic acid cycle and oxidative phosphorylation) pathway genes were the most downregulated and most significantly correlated with sarcopenia. Also, perturbed were genes/pathways associated with neuromuscular junction patency (providing molecular evidence of sarcopenia-related functional denervation and neuromuscular junction remodeling), protein degradation, and inflammation. Proteomic analysis of samples at 6, 18, and 27 months confirmed the depletion of mitochondrial energy metabolism proteins and neuromuscular junction proteins. Together, these findings suggest that therapeutic approaches that simultaneously stimulate mitochondrogenesis and reduce muscle proteolysis and inflammation have potential for treating sarcopenia. 相似文献
945.
Alessandra Ciprandi Wanderson Marques da Silva Agenor Valadares Santos Adriano Monteiro de Castro Pimenta Marta Sofia Peixe Carepo Maria Paula Cruz Schneider Vasco Azevedo Artur Silva 《Current microbiology》2013,67(1):100-106
Chromobacterium violaceum is a beta-proteobacterium with high biotechnological potential, found in tropical environments. This bacterium causes opportunistic infections in both humans and animals, that can spread throughout several tissues, quickly leading to the death of the host. Genomic studies identified potential mechanisms of pathogenicity but no further studies were done to confirm the expression of these systems. In this study 36 unique protein entries were identified in databank from a two-dimensional profile of C. violaceum secreted proteins. Chromobacterium violaceum exoproteomic preliminary studies confirmed the production of proteins identified as virulence factors (such as a collagenase, flagellum proteins, metallopeptidases, and toxins), allowing us to better understand its pathogenicity mechanisms. Biotechnologically interesting proteins (such as chitinase and chitosanase) were also identified among the secreted proteins, as well as proteins involved in the transport and capture of amino acids, carbohydrates, and oxidative stress protection. Overall, the secreted proteins identified provide us important insights on pathogenicity mechanisms, biotechnological potential, and environment adaptation of C. violaceum. 相似文献
946.
Osvaldo D. Castelán-Martínez Carlos Hoyo-Vadillo Emmanuel Sandoval-García Lucila Sandoval-Ramírez Miriam González-Ibarra Gloria Solano-Solano Rita A. Gómez-Díaz Esteban J. Parra Miguel Cruz Adán Valladares-Salgado 《Gene》2013
Allele frequency differences of functional CYP2C9 polymorphisms are responsible for some of the variation in drug response observed in human populations. The most relevant CYP2C9 functional variants are CYP2C9*2 (rs1799853) and CYP2C9*3 (rs1057910). These polymorphisms show variation in allele frequencies among different population groups. The present study aimed to analyze these polymorphisms in 947 Mexican-Mestizo from Mexico City and 483 individuals from five indigenous Mexican populations: Nahua, Teenek, Tarahumara, Purepecha and Huichol. The CYP2C9*2 allele frequencies in the Mestizo, Nahua and Teenek populations were 0.051, 0.007 and 0.005, respectively. As for CYP2C9*3, the allelic frequencies in the Mestizo, Nahua and Teenek populations were 0.04, 0.005 and 0.005, respectively. The CYP2C9*2 and CYP2C9*3 alleles were not observed in the Tarahumara, Purepecha and Huichol populations. These findings are in agreement with previous studies reporting very low allele frequencies for these polymorphisms in American Indigenous populations. 相似文献
947.
H. Ribeiro S. Morales C. Salmerón A. Cruz L. Calado M. I. Rodríguez-García J. D. Alché I. Abreu 《Aerobiologia》2013,29(4):513-521
Olive pollen presents intercultivar variability as regards to its antigenic and allergenic composition. In this study, we report the presence of differences among the SDS-PAGE pollen protein profiles of twelve Portuguese olive cultivars. Though most soluble proteins from these extracts seemed similar, three bands of about 18, 20 and 22 kDa presented sharp differences in intensity among the cultivars analyzed. The dissimilarity of patient’s sera reactivity to these protein extracts and the presence of several allergens already characterized (Ole e 1, Ole e 2, Ole e 5 and Ole e 9) in the extracts were also investigated. Epidemiological data indicated that 53.3 % out of the 428 patients analyzed with reactivity to pollen extracts, presented specific IgE levels to Olea europaea. A representative number of these sera were assayed in immunoblotting experiments. The cultivars ‘Galega’ and ‘Conserva de Elvas’ displayed low reactivity to the sera of atopic patients, whereas the extracts corresponding to the cultivars ‘Cobrançosa’, ‘Ascolana’ and ‘Verdeal de Serpa’ led to higher IgE reactivity. The use of antibodies to the allergens Ole e 1, Ole e 2, Ole e 5 and Ole e 9 in immunoblotting experiments also allowed cultivar discrimination. The cultivar ‘Verdeal de Serpa’ presented the highest Ole e 1, Ole e 5 and Ole e 9 allergen loads but the lowest Ole e 2. ‘Carrasquenha’ was the second cultivar in terms of the higher allergen content. Oppositely, the lowest allergen loads were those of the cultivars ‘Galega’ and ‘Conserva de Elvas’ coincidentally with their low IgE reactivity. These data may help interpret physiological differences in pollen performance for successful olive fertilization and, moreover, to better define future strategies for allergy diagnosis and treatment by specific immunotherapy. 相似文献
948.
Eunice Ramirez Anthony Cruz Diana Rodriguez Lilen Uchima Ruth Pietri Alberto Santana 《Molecular simulation》2013,39(7):715-725
Haemoglobin I from Lucina pectinata is a monomeric protein consisting of 142 amino acids. Its active site contains a peculiar arrangement of phenylalanine residues (PheB10, PheCD1 and PheE11) and a distal Gln at position E7. Active site mutations at positions B10, E7 and E11 were performed in deoxy haemoglobin I (HbI), followed by 10 ns molecular dynamic simulations. The results showed that the mutations induced changes in domains far from the active site producing more flexible structures than the native HbI. Distance analyses revealed that the heme pocket amino acids at positions E7 and B10 are extremely sensitive to any heme pocket residue mutation. The high flexibility observed by the E7 position suggests an important role in the ligand binding kinetics in ferrous HbI, while both positions play a major role in the ligand stabilisation processes. Furthermore, our results showed that E11Phe plays a pivotal role in protein stability. 相似文献
949.
950.
Asun Rodríguez‐Ua Vernica Cruz‐Alonso Zoë Rohrer Loreto Martínez‐Baroja 《Restoration Ecology》2020,28(1):12-15
Restoration ecology is a young scientific discipline whose limitations can compromise the recovery of ecosystem biodiversity and functions. Specifically for limitations on forest restoration, we first recommend considering measures prior to land use changes to deal with the common lack of efforts to anticipate and plan restoration. Second, we suggest using multiple references in restoration planning to avoid simplified reference characterization, and we advise assessing ecosystem recovery with indicators that better incorporate ecosystem complexity in recovery assessments. Finally, we propose initiatives to encourage scientific communication outside academia to diminish the communication gap between scientists and practitioners. 相似文献