Summer dispersal and survival of juvenile Great Tits in southern Sweden

Summary Movements and survival of 506 first-brood Great Tit nestlings that fledged in mid-June in an oak wood in southern Sweden were studied by intensive trapping in that wood and in several neighbouring woods between 17 July and 10 September 1977. A total of 1177 captures of 508 individuals was made.Two periods of summer dispersal can be distinguished: the first period, one to one- and-one-half months after fledging, lasts longer in females and results in females moving farther away from their birthplace than males; and the second period in early September. There was no effect of brood-size, fledging date or size on dispersal movements. There is, however, a nest effect in that siblings tend to be more alike in the distance moved than non-siblings.The summer mortality rate is constant but high (13% per week). It is not affected by brood size or fledging date, but during the first month after fledging large individuals survive better than smaller ones. The summer mortality rate of dispersers (birds moving between woods) is not higher than that of non-dispersers.The results contradict two hypotheses proposed to explain when and how postfledging mortality occurs. The mortality rate is not higher during the first month after fledging, as Perrins' and Lack's hypothesis predicts. Since only 22% of the young are still alive at the beginning of September, autumn territorial behaviour cannot be the main factor causing juvenile losses, as proposed by Kluyver.There is some circumstantial evidence that Great Tits compete for food during the summer, and that food therefore could be in short supply, as suggested by Perrins.The observed differential dispersal of adults and young, and of male and female juveniles, may be the result of the dominance relationships in the family flock and later in the summer flocks, with subordinate individuals moving farthest.     

Xanthones et C-glucosides flavoniques du genre Gentiana (section Cyclostigma)

Xanthones with a uniform 1,3,7,8-oxidation pattern, the C-glycosides isoorientin and isovitexin, and in some cases mangiferin, were isolated from the aerial parts of G. nivalis, G. brachyphylla, G. favrati, G. rostani, G. utriculosa and G. schleicheri Kunz. The distribution of these compounds within the section Cyclostigma is given. Comparison of phenolic patterns in other sections of Gentiana is made.     

Absence of Detectable Mitochondrial Recombination in Paramecium

An extensive search for recombination between mitochondrial markers was carried out in Paramecium tetraurelia. Thirty-two combinations, altogether involving 24 different markers, were studied. The markers belonged to the three main categories of mitochondrial mutations presently available in this organism, (a) Spontaneous or UV-induced antibiotic resistance mutations, most probably affecting mitochondrial ribosomes, (b) nitrosoguanidine-induced antibiotic resistance markers displaying thermosensitivity or slow growth, enabling easy selection of possible wild-type recombinants, and (c) mitochondrial partial suppressors of a nuclear gene, probably corresponding to molecular alterations distinct from the preceding two categories. In addition, different genetic configurations were analyzed (i.e., mutant X mutant, double-mutant X wild-type, etc.).--None of the combinations yielded any evidence for the occurrence of recombined genomes despite the fact that: (1) all of them were studied on a large scale involving the screening of at least several thousand mitochondrial genomes (often several millions), (2) in many of them the detection level was sufficiently high to enable the isolation of spontaneous mutants in control cells, and (3) in several of them, reconstitution experiments carried out in parallel show that the conditions were fully adequate to detect recombinant genotypes. The results are in marked contrast with those obtained on the few other organisms in which mitochondrial recombination has been studied, particularly Saccharomyces cerevisiae, in which mitochondrial recombination is intense.--The most likely basis for the various manifestations of mitochondrial genetic autonomy in Paramecium, described in this as well as in previous publications, is that the chondriome of this organism is made up of thousands of structurally discrete, noninteracting units.     

Synchronous growth of a freshwater diatom Melosira italica under natural environment

Summary A filamentous diatom Melosira italica was collected at the beginning of rainy season from a shallow lake in the tropical savanna region in Brazil. Even the sample taken from surface water contained empty cells in high percentages. The number of cells per filament of M. italica showed a peculiar pulse-like frequency distribution with peak values at 4, 8, 12 and 16. Evidences of the synchronous cell division in this planktonic diatom under natural environment are discussed.     

Ultrastructural alteration of plant plasma membranes induced by auxin and calcium ions

Ultrastructural changes in isolated and in situ plasma membranes of etiolated soybean hypocotyls (Glycine max L. cv. Wayne) were induced by indole-3-acetic acid (IAA), other auxins, and calcium chloride. Fixed and embedded preparations were stained by a phosphotungstate-chromate procedure to identify and accentuate plasma membrane. Measurements were on micrographs obtained with an electron optical system calibrated and corrected for reproducible and accurate size measurements. Plasma membranes treated for 20 minutes with 1 mum IAA were 10 to 15% thinner than controls. The response to IAA was rapid, reproducible, auxin-specific, temperature-dependent, and reversible. Comparable responses were obtained with isolated and in situ membranes. Membranes treated with 0.5 m calcium chloride for 20 minutes were 15 to 20% thicker than controls. Multiple cycles of alternating calcium and IAA treatments yielded membranes with dimensions that reflected the last treatment of the series. The findings show a direct response of plasma membranes to growth regulating agents and provide evidence for a cell-free response of isolated plasma membranes to a hormone.     

La criocerine: Nouvel alcaloide isole des tiges de Crioceras dipladeniiflorus

Seven indolic alkaloids have been isolated from the stem bark of Crioceras dipladeniiflorus. One of them is new and has been called criocerine. Its structure, 7, has been established by use of physical data and chemical correlation with [Δ¹⁴]-vincamine.     

Outer pore topology of the ECaC-TRPV5 channel by cysteine scan mutagenesis

The substituted cysteine accessibility method (SCAM) was used to map the external vestibule and the pore region of the ECaC-TRPV5 calcium-selective channel. Cysteine residues were introduced at 44 positions from the end of S5 (Glu515) to the beginning of S6 (Ala560). Covalent modification by positively charged MTSET applied from the external medium significantly inhibited whole cell currents at 15/44 positions. Strongest inhibition was observed in the S5-linker to pore region (L520C, G521C, and E522C) with either MTSET or MTSES suggesting that these residues were accessible from the external medium. In contrast, the pattern of covalent modification by MTSET for residues between Pro527 and Ile541 was compatible with the presence of a alpha-helix. The absence of modification by the negatively charged MTSES in that region suggests that the pore region has been optimized to favor the entrance of positively charged ions. Cysteine mutants at positions -1, 0, +1, +2 around Asp542 (high Ca2+ affinity site) were non-functional. Whole cell currents of cysteine mutants at +4 and +5 positions were however covalently inhibited by external MTSET and MTSES. Altogether, the pattern of covalent modification by MTS reagents globally supports a KcsA homology-based three-dimensional model whereby the external vestibule in ECaC-TRPV5 encompasses three structural domains consisting of a coiled structure (Glu515 to Tyr526) connected to a small helical segment of 15 amino acids (527PTALFSTFELFLT539) followed by two distinct coiled structures Ile540-Pro544 (selectivity filter) and Ala545-Ile557 before the beginning of S6.