Effect of starting stance on initial sprint performance

The effect of different starting stances from a standing position on short sprint times and the subsequent variability in times was investigated in this study. A dual-beam timing light system was used to measure 5- and 10-m times for 3 different standing starts commonly found in the sporting environment: parallel (feet parallel to the start line), split (lead left foot on start line, right leg back), and false (initial parallel start, right leg drops back to split start when movement initiated). The parallel start was found to be significantly (alpha < 0.05) slower than the other 2 stances for both the 5- ( approximately 8.3%) and the 10-m (approximately 5.9%) distances. Within the trial, variation of the different starting stances was equally consistent; however, there was less variability for the 10-m distance (CV = 1.16-1.67%) than the 5-m distance (CV = 1.43-2.15%) for each start for both men and women. The split and false start seem to offer the best option as a movement strategy for minimizing short-distance sprint times. However, the benefits of these 2 starts are less clear if total movement time is the variable of interest.     

Free radicals and antioxidants in normal physiological functions and human disease

Reactive oxygen species (ROS) and reactive nitrogen species (RNS, e.g. nitric oxide, NO(*)) are well recognised for playing a dual role as both deleterious and beneficial species. ROS and RNS are normally generated by tightly regulated enzymes, such as NO synthase (NOS) and NAD(P)H oxidase isoforms, respectively. Overproduction of ROS (arising either from mitochondrial electron-transport chain or excessive stimulation of NAD(P)H) results in oxidative stress, a deleterious process that can be an important mediator of damage to cell structures, including lipids and membranes, proteins, and DNA. In contrast, beneficial effects of ROS/RNS (e.g. superoxide radical and nitric oxide) occur at low/moderate concentrations and involve physiological roles in cellular responses to noxia, as for example in defence against infectious agents, in the function of a number of cellular signalling pathways, and the induction of a mitogenic response. Ironically, various ROS-mediated actions in fact protect cells against ROS-induced oxidative stress and re-establish or maintain "redox balance" termed also "redox homeostasis". The "two-faced" character of ROS is clearly substantiated. For example, a growing body of evidence shows that ROS within cells act as secondary messengers in intracellular signalling cascades which induce and maintain the oncogenic phenotype of cancer cells, however, ROS can also induce cellular senescence and apoptosis and can therefore function as anti-tumourigenic species. This review will describe the: (i) chemistry and biochemistry of ROS/RNS and sources of free radical generation; (ii) damage to DNA, to proteins, and to lipids by free radicals; (iii) role of antioxidants (e.g. glutathione) in the maintenance of cellular "redox homeostasis"; (iv) overview of ROS-induced signaling pathways; (v) role of ROS in redox regulation of normal physiological functions, as well as (vi) role of ROS in pathophysiological implications of altered redox regulation (human diseases and ageing). Attention is focussed on the ROS/RNS-linked pathogenesis of cancer, cardiovascular disease, atherosclerosis, hypertension, ischemia/reperfusion injury, diabetes mellitus, neurodegenerative diseases (Alzheimer's disease and Parkinson's disease), rheumatoid arthritis, and ageing. Topics of current debate are also reviewed such as the question whether excessive formation of free radicals is a primary cause or a downstream consequence of tissue injury.     

Aspidosperma (Apocynaceae) plant cytotoxicity and activity towards malaria parasites. Part II: experimental studies with Aspidosperma ramiflorum in vivo and in vitro

Several species of Aspidosperma plants are used to treat diseases in the tropics, including Aspidosperma ramiflorum, which acts against leishmaniasis, an activity that is experimentally confirmed. The species, known as guatambu-yellow, yellow peroba, coffee-peroba andmatiambu, grows in the Atlantic Forest of Brazil in the South to the Southeast regions. Through a guided biofractionation of A. ramiflorum extracts, the plant activity against Plasmodium falciparum was evaluated in vitro for toxicity towards human hepatoma G2 cells, normal monkey kidney cells and nonimmortalised human monocytes isolated from peripheral blood. Six of the seven extracts tested were active at low doses (half-maximal drug inhibitory concentration < 3.8 µg/mL); the aqueous extract was inactive. Overall, the plant extracts and the purified compounds displayed low toxicity in vitro. A nonsoluble extract fraction and one purified alkaloid isositsirikine (compound 5) displayed high selectivity indexes (SI) (= 56 and 113, respectively), whereas compounds 2 and 3 were toxic (SI < 10). The structure, activity and low toxicity of isositsirikine in vitro are described here for the first time in A. ramiflorum, but only the neutral and precipitate plant fractions were tested for activity, which caused up to 53% parasitaemia inhibition of Plasmodium berghei in mice with blood-induced malaria. This plant species is likely to be useful in the further development of an antimalarial drug, but its pharmacological evaluation is still required.     

The reliability of linear position transducer and force plate measurement of explosive force-time variables during a loaded jump squat in elite athletes

The best method of assessing muscular force qualities during isoinertial stretch shorten cycle lower body movements remains a subject of much debate. This study had 2 purposes: Firstly, to calculate the interday reliability of peak force (PF) measurement and a variety of force-time measures, and, secondly, to compare the reliability of the 2 most common technologies for measuring force during loaded jump squats, the linear position transducer (PT), and the force plate (FP). Twenty-five male elite level rugby union players performed 3 rebound jump squats with a 40-kg external load on 2 occasions 1 week apart. Vertical ground reaction forces (GRFs) were directly measured via an FP, and force was differentiated from position data collected using a PT. From these data, a number of force-time variables were calculated for both the FP and PT. Intraclass correlation coefficient (ICC), coefficient of variation (CV), and percent change in the mean were used as measures of between-session reliability. Additionally, Pearson's product moment correlation coefficients were used to investigate intercorrelations between variables and technologies. Both FP and PT were found to be a reliable means of measuring PF (ICC = 0.88-0.96, CV = 2.3-4.8%), and the relationship between the 2 technologies was very high and high for days 1 and 2, respectively (r = 0.67-0.88). Force-time variables calculated from FP data tended to have greater relative and absolute consistency (ICC = 0.70-0.96, CV = 5.1-51.8%) than those calculated from differentiated PT data (ICC = 0.18-0.95, CV = 7.7-93.6%). Intercorrelations between variables ranged from trivial to practically perfect (r = 0.00-1.00). It was concluded that PF can be measured reliably with both FP and PT technologies, and these measurements are related. A number of force-time values can also be reliably calculated via the use of GRF data. Although some of these force-time variables can be reliably calculated using position data, variation of measurement is generally greater when using position data to calculate force.     

Characterization of an oxidoreductase from the arylamine N-acetyltransferase operon in Mycobacterium smegmatis

Mycobacterium?tuberculosis, the most successful bacterial pathogen, causes tuberculosis, a disease that still causes more than 2 million deaths per year. Arylamine N-acetyltransferase is an enzyme that is conserved in most Mycobacterium spp. The nat gene belongs to an operon that is important for the intracellular survival of M. tuberculosis within macrophages. The nat operon in Mycobacterium smegmatis and other fast-growing mycobacterial species has a unique organization containing genes with uncharacterized function. Here, we describe the biochemical, biophysical and structural characterization of the MSMEG_0308 gene product (MS0308) of the M. smegmatis nat operon. While characterizing the function of MS0308, we validated the oxidoreductase property; however, we found that the enzyme was not utilizing dihydrofolate as its substrate, hence we first report that MS0308 is not a dihydrofolate reductase, as annotated in the genome. The structure of this oxidoreductase was solved at 2.0 ? in complex with the cofactor NADPH and has revealed the hydrophobic pocket where the endogenous substrate binds.     

Bicycle seat interface pressure: reliability, validity, and influence of hand position and workload

Bicycle seat pressure is often examined by researchers exploring solutions for reducing seat injuries as it is thought a critical determinant; however, a reliable and valid methodology for this undertaking has not been reported. The current study was designed to address this shortcoming and to establish baseline interface pressure measurements for females and males. Participants completed two separate identical bicycle ergometer trials at 118 W in the tops and drops and at 300+/-82.4 W in top handlebar positions. Seat pressures were quantified from a pressure-sensing mat and the validity of the pressure system was examined through the relationship between known seat weights and the sum of seat pressures. Within trial intraclass correlation coefficients (ICCs) ranged between 0.90 and 0.99 and the between trial ICC values ranged between 0.02 and 0.96. The relationship (Pearson correlation coefficient) between seat weight and the sum of pressures was 0.97. Significant peak pressures were 39% greater in the 118 W top than 300 W top handlebar condition and 29% greater than the 118 W drop handlebar condition (p < 0.05). The gender comparison revealed that significant male peak pressure values were greater (24%) and that female values were less influenced by the hand position factor. Select within trial pressure measurements were found to be reliable during pedaling and valid statically. Additionally, the results indicated that workrate and hand position were factors influencing seat pressure and that males and females responded differently to adjustments of these factors.     

Variation in oxytocin is related to variation in affiliative behavior in monogamous, pairbonded tamarins

Oxytocin plays an important role in monogamous pairbonded female voles, but not in polygamous voles. Here we examined a socially monogamous cooperatively breeding primate where both sexes share in parental care and territory defense for within species variation in behavior and female and male oxytocin levels in 14 pairs of cotton-top tamarins (Saguinus oedipus). In order to obtain a stable chronic assessment of hormones and behavior, we observed behavior and collected urinary hormonal samples across the tamarins’ 3-week ovulatory cycle. We found similar levels of urinary oxytocin in both sexes. However, basal urinary oxytocin levels varied 10-fold across pairs and pair-mates displayed similar oxytocin levels. Affiliative behavior (contact, grooming, sex) also varied greatly across the sample and explained more than half the variance in pair oxytocin levels. The variables accounting for variation in oxytocin levels differed by sex. Mutual contact and grooming explained most of the variance in female oxytocin levels, whereas sexual behavior explained most of the variance in male oxytocin levels. The initiation of contact by males and solicitation of sex by females were related to increased levels of oxytocin in both. This study demonstrates within-species variation in oxytocin that is directly related to levels of affiliative and sexual behavior. However, different behavioral mechanisms influence oxytocin levels in males and females and a strong pair relationship (as indexed by high levels of oxytocin) may require the activation of appropriate mechanisms for both sexes.