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571.
Simone C 《Autophagy》2007,3(5):468-471
Autophagy is a vacuolar process leading to the degradation of long-lived proteins and cytoplasmic organelles in eukaryotes. This process has an important role in normal and cancer cells during adaptation to changing environmental conditions, cellular and tissue remodeling, and cell death. To date, several signaling cascades have been described to regulate autophagy in a cell type-specific and signal-dependent manner. We found that pharmacological blockade of the p38 pathway in colorectal cancer cells, either by the inhibitor SB202190 or by genetic ablation of p38 alpha kinase, causes cell cycle arrest and autophagic cell death. In these cells, a complex network of intracellular kinase cascades controls autophagy and survival since the effect of p38 alpha blockade is differentially affected by the pharmacological inhibition of MEK1, PI(3)K class I and III, and mTOR or by the differentiation status. Collectively, our results suggest an opportunity for exploiting the pharmacological manipulation of the p38 alpha pathway in the treatment of colorectal cancer. Given the number of drugs, currently available or under development, that target the p38 pathway, it stands to reason that elucidating the molecular mechanisms that link p38 and autophagy might have an impact on the clinical translation of these drugs. 相似文献
572.
Marina Barilani Athanassios Sfougaris Alexis Giannakopoulos Nadia Mucci Cristiano Tabarroni Ettore Randi 《Conservation Genetics》2007,8(2):343-354
The nominal subspecies of rock partridge (Alectoris graeca graeca) is widely distributed in Greece, where populations are declining due to over-hunting and habitat changes. Captive-reared
chukars (A. chukar) have been massively released throughout the country, raising fear that introgressive hybridisation might have disrupted
local adaptations leading to further population declines. In this study we used mtDNA control-region sequences and Bayesian
admixture analyses of multilocus genotypes determined at eight microsatellite loci, to assess the extent of introgressive
hybridisation in 319 wild rock partridges collected in Greece. A neighbour-joining tree split the mtDNA haplotypes into three
strongly supported clades, corresponding to rock, red-legged (A.␣rufa) and chukar partridges. We did not detect any case of maternal introgression. In contrast, admixture analyses of microsatellite
genotypes identified from four to 28 putative hybrids (according to different assignment criteria), corresponding to 1.2–8.8%
of the samples, which were widespread throughout all the country. Power and limits of admixture analyses were assessed using
simulated hybrid genotypes, which revealed that a small number of markers can detect all first and second generation hybrids
(F
1 and F
2), and up to 90% of the first generation backrossess. Thus, the true proportion of recently introgressed rock partridges in
Greece might be ca. 20%. These findings indicate that introgressive hybridisation is widespread, suggesting that released
captive-bred partridges have reproduced and hybridised in nature polluting the gene pool of wild rock partridge populations
in Greece. 相似文献
573.
Vendruscolo Raquel Guidetti Fagundes Mariane Bittencourt Maroneze Mariana Manzoni do Nascimento Tatiele Casagrande de Menezes Cristiano Ragagnin Barin Juliano Smanioto Zepka Leila Queiroz Jacob-Lopes Eduardo Wagner Roger 《Bioprocess and biosystems engineering》2019,42(5):727-739
Bioprocess and Biosystems Engineering - Environmental factors directly affect the growth and composition of microalgal biomass. Therefore, the present work analyzed the metabolomics (amino acids,... 相似文献
574.
José Wellington Alves dos Santos Cristiano Feijó Andrade Tiago Silveira de Araújo Lopes Alberto Thomaz Londero 《Mycopathologia》1996,134(3):135-136
A case of unilateral paracoccidioidal pulmonary lesion simulating a neoplasm is reported.This case was presented on the XVII World Congress on Diseases of the Chest, Amsterdam, The Netherland, June 1993. 相似文献
575.
Nuna C.P. Araújo Victoria Barton Michael Jones Paul A. Stocks Stephen A. Ward Jill Davies Patrick G. Bray Alison E. Shone Maria L.S. Cristiano Paul M. O’Neill 《Bioorganic & medicinal chemistry letters》2009,19(7):2038-2043
A novel series of semi-synthetic trioxaquines and synthetic trioxolaquines were prepared, in moderate to good yields. Antimalarial activity was evaluated against both the chloroquine-sensitive 3D7 and resistant K1 strain of Plasmodium falciparum and both series of compounds were shown to be active in the low nanomolar range. For comparison the corresponding 9-amino acridine analogues were also prepared and shown to have low nanomolar activity like their quinoline counterparts. 相似文献
576.
Calistri A Del Vecchio C Salata C Celestino M Celegato M Göttlinger H Palù G Parolin C 《Journal of cellular physiology》2009,218(1):175-182
RNA-enveloped viruses bud from infected cells by exploiting the multivesicular body (MVB) pathway. In this context, ubiquitination of structural viral proteins and their direct interaction with cellular factors involved in the MVB biogenesis through short proline rich regions, named late domains (L-domains), are crucial mechanisms. Here we report that, in contrast with the human immunodeficiency virus (HIV), the feline immunodeficiency virus (FIV), a non-primate lentivirus, is strictly dependent for its budding on a "PSAP"-type L-domain, mapping in the carboxy-terminal region of Gag, irrespective of a functional viral protease. Moreover, we provide evidence that FIV egress is related to Gag ubiquitination, that is, linked to the presence of an active L-domain. Finally, although FIV Gag does not contain a PPxY motif, we show that the Nedd4-2s ubiquitin ligase enhances FIV Gag ubiquitination and it is capable to rescue viral mutants lacking a functional L-domain. In conclusion, our data bring to light peculiar aspects of FIV egress, but we also demonstrate that a non-primate lentivirus shares with HIV-1 a novel mechanism of connection to the cellular budding machinery. 相似文献
577.
Elena Sartori Cristiano Salata Arianna Calistri Giorgio Palù Cristina Parolin 《Journal of cellular physiology》2009,219(3):797-799
The 8th annual meeting of the Italian Society of Virology (SIV) took place in Orvieto, Italy from the 21st to the 23rd of September 2008. The meeting covered different areas of Virology and the scientific sessions focused on: general virology and viral genetics; viral oncology, virus–host interaction and pathogenesis; emerging viruses and zoonotic, foodborne and environmental pathways of transmission; viral immunology and vaccines; viral biotechnologies and gene therapy; medical virology and antiviral therapy. The meeting had an attendance of about 160 virologists from all Italy. In this edition, a satellite workshop on “Viral biotechnologies” was organized in order to promote the role of virologists in the biotechnological research and teaching fields. A summary of the plenary lectures and oral selected presentations is reported. J. Cell. Physiol. 219: 797–799, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
578.
579.
Deise Juliana Kolling Jean Borges Bertoldo Fábio Cristiano Angonesi Brod Javier Vernal Hernán Terenzi Ana Carolina Maisonnave Arisi 《Molecular biotechnology》2010,46(2):168-175
Staphylococcus xylosus AF208229 lipase was expressed in E. coli containing an histidine-tag (WT-Val). In the present work, in order to check the importance of the residue 309 in the specific
activity, the amino acid side chain residue valine 309 was substituted by aspartate or lysine through site-directed mutagenesis.
Both mutant lipases (MUT-Lys and MUT-Asp) were expressed in E. coli and the recombinant histidine-tagged lipases were purified by immobilized metal ion affinity chromatography. The enzyme activity
was determined using p-nitrophenyl butyrate as substrate and secondary structure content was evaluated by circular dichroism. MUT-Lys and MUT-Asp
presented significant increase of lipase activity (P < 0.05) in comparison to WT-Val, although highest activities for the three enzymes were observed at the same pH and temperature
(pH 9.0 and 42°C). The wild type and mutant lipases presented high thermal stability, after 30 min of incubation at 80°C all
enzymes retained their initial activities. 相似文献
580.
The aim of the present study was to evaluate pharmacological and toxicological properties of 1-buthyltelurenyl-2-methylthioheptene (compound 1). In vitro, compound 1 at 1 μM was effective in reducing lipid peroxidation induced by Fe/EDTA. Compound 1 presented neither thiol peroxidase nor thiol oxidase activity and did not change δ-ALA-D (δ-aminolevulinate dehydratase) activity (10-400 μM). Calculated LD50 of compound 1, administered by oral route, was 65.1 μmol/kg. Rats treated with compound 1 did not reveal any motor impairment in the open field. Hepatic, renal and cerebral lipid peroxidation in treated rats did not differ from those in control rats. Conversely, 0.5 μmol/kg of compound 1 decreased lipid peroxidation in spleen. δ-ALA-D activity in liver and spleen was inhibited in rats treated with the higher dose of compound 1 but no significant differences were detected in renal δ-ALA-D activity. AST (aspartate aminotransferase) and ALT (alanine aminotransferase) activities as well as urea and creatinine levels were increased by high doses of compound 1 (50-75 μmol/kg). Compound 1 induced a significant decrease in plasma triglyceride levels but none of the doses tested changed the cholesterol level. This is a promising compound for more detailed pharmacological studies involving organotellurium compounds. 相似文献