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11.
Palumbo ML Fosser NS Rios H Zorrilla Zubilete MA Guelman LR Cremaschi GA Genaro AM 《Journal of neurochemistry》2007,102(1):261-274
Nitric oxide (NO) has been involved in many pathophysiological brain processes. However, the exact role of NO in the cognitive deficit associated to chronic stress exposure has not been elucidated. In this study, we investigated the participation of hippocampal NO production and their regulation by protein kinase C (PKC) in the memory impairment induced in mice subjected to chronic mild stress model (CMS). CMS mice showed a poor learning performance in both open field and passive avoidance inhibitory task respect to control mice. Histological studies showed a morphological alteration in the hippocampus of CMS mice. On the other hand, chronic stress induced a diminished NO production by neuronal nitric oxide synthase (nNOS) correlated with an increment in gamma and zeta PKC isoenzymes. Partial restoration of nNOS activity was obtained after PKC activity blockade. NO production by inducible nitric oxide synthase isoform was not detected. The magnitude of oxidative stress, evaluated by reactive oxygen species production, after excitotoxic levels of NMDA was increased in hippocampus of CMS mice. Moreover, ROS formation was higher in the presence of nNOS inhibitor in both control and CMS mice. Finally, treatment of mice with nNOS inhibitors results in behavioural alterations similar to those observed in CMS animals. These findings suggest a novel role for nNOS showing protective activity against insults that trigger tissue toxicity leading to memory impairments. 相似文献
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15.
Giuliano Meyer Guido Bottà Carlo Rossetti Dario Cremaschi 《The Journal of membrane biology》1990,118(2):107-120
Summary In rabbit gallbladder epithelium, a Na+/H+, Cl–/HCO
3
–
double exchange and a Na+–Cl– symport are both present, but experiments on intact tissue cannot resolve whether the two transport systems operate simultaneously. Thus, isolated apical plasma membrane vesicles were prepared. After preloading with Na+, injection into a sodium-free medium caused a stable intravesicular acidification (monitored with the acridine orange fluorescence quenching method) that was reversed by Na+ addition to the external solution. Although to a lesser extent, acidification took place also in experiments with an electric potential difference (PD) equal to 0. If a preset pH difference (pH) was imposed ([H+]in>[H+]out, PD=0), the addition of Na-gluconate to the external solution caused pH dissipation at a rate that followed saturation kinetics. Amiloride (10–4
m) reduced the pH dissipation rate. Taken together, these data indicate the presence of Na+ and H+ conductances in addition to an amiloride-sensitive, electroneutral Na+/H+ exchange.An inwardly directed [Cl–] gradient (PD=0) did not induce intravesicular acidification. Therefore, in this preparation, there was no evidence for the presence of a Cl–/OH– exchange.When both [Na+] and [Cl–] gradients (outwardly directed, PD=0) were present, fluorescence quenching reached a maximum 20–30 sec after vesicle injection and then quickly decreased. The decrease was not observed in the presence of a [Na+] gradient alone or the same [Na+] gradient with Cl– at equal concentrations at both sides. Similarly, the decrease was abolished in the presence of both Na+ and Cl– concentration gradients and hydrochlorothiazide (5×10–4
m). The decrease was not influenced by an inhibitor of Cl–/OH– exchange (10–4
m furosemide) or of Na+–K+–2Cl– symport (10–5
m bumetanide).We conclude that a Na+/H+ exchange and a Na+–Cl– symport are present and act simultaneously. This suggests that in intact tissue the Na+–Cl– symport is also likely to work in parallel with the Na+/H+ exchange and does not represent an induced homeostatic reaction of the epithelium when Na+/H+ exchange is inhibited. 相似文献
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17.
Maria Laura Barreiro Arcos Gabriela Gorelik Alicia Klecha Nora Goren Cristina Cerquetti Graciela A Cremaschi 《Nitric oxide》2003,8(2):111-118
Nitric oxide (NO)-derived from T lymphocytes in an autocrine fashion can modulate events in the cell. However, the exact role of NO on the control of lymphocyte growth is controversial since both stimulation and inhibition have been demonstrated. Nitric oxide synthase (NOS) activity in normal and tumor T lymphocyte proliferation was studied here. Resting normal T lymphocytes displayed low levels of NOS activity that were slightly increased upon mitogenic stimulation. In contrast, BW5147 T lymphoma cells displayed higher basal levels than normal T lymphocytes that were significantly augmented when induced to proliferate. This activity was slightly modified in the presence of the calcium chelator EGTA and was blocked by competitive and irreversible NOS inhibitors, as well as by selective blockers of iNOS. Furthermore, tumor but not normal cell proliferation was impaired by NOS and iNOS blockers, while a calcium blocker only affected normal cell growth. iNOS expression, both at the protein and at the mRNA levels, was demonstrated on growing BW5147 cells but not on arrested tumor or normal lymphocytes. The contribution of iNOS to sustained proliferation of tumor cells is discussed. 相似文献
18.
Valeria Ayelli Edgar Ana Mara Genaro Graciela Cremaschi Leonor Sterin-Borda 《Cellular signalling》1998,10(10):721-726
The present study was undertaken to analyse the effect of fluoxetine upon murine T-lymphocyte proliferation. We found that fluoxetine exerted a dual effect, which depended on the degree of lymphocyte activation: at mitogenic concentration (2 μg/mL) of concavalin A (Con A), we observed an inhibitory effect on cellular proliferation, whereas, on submitogenic Con A concentration (1 μg/mL), fluoxetine stimulated the cellular response. Given these facts, we studied PKC activation and calcium mobilisation in both stimulatory and inhibitory effects of fluoxetine on T-cell proliferation. We observed that fluoxetine increased PKC translocation obtained with 1 μg/mL Con A concentration, whereas PKC was degraded when 2 μg/mL was used. This mechanism is thought to be mediated by calcium mobilisation. According to our results, fluoxetine seemed to modulate calcium influx, which, in turn, would influence PKC translocation, modulating the immune response. 相似文献
19.
Summary Bicarbonate presence in the bathing media doubles Na+ and fluid transepithelial transport and in parallel significantly increases Na+ and Cl– intracellular concentrations and contents, decreases K+ cell concentration without changing its amount, and causes a large cell swelling. Na+ and Cl– lumen-to-cell influxes are significantly enhanced, Na+ more so than Cl–. The stimulation does not raise any immediate change in luminal membrane potential and cannot be due to a HCO
3
–
-ATPase in the brush border. The stimulation goes together with a large increase in a Na+-dependent H+ secretion into the lumen. All of these data suggests that HCO
3
–
both activates Na+–Cl– cotransport and H+–Na+ countertransport at the luminal barrier.Thiocyanate inhibits Na+ and fluid transepithelial transport without affecting H+ secretion and HCO
3
–
-dependent Na+ influx. It reduces Na+ and Cl– concentrations and contents, increases the same parameters for K+, causes a cell shrinking, and abolishes the lumen-to-cell Cl– influx. It enters the cell and is accumulated in the cytoplasm with a process which is Na+-dependent and HCO
3
–
-activated. Thus, SCN– is likely to compete for the Cl– site on the cotransport carrier and to be slowly transferred by the cotransport system itself. 相似文献
20.
Dario Cremaschi Silvio Hénin Giuliano Meyer Tiziana Bacciola 《The Journal of membrane biology》1977,34(1):55-71
Summary When amphotericin B is added to the medium bathing the luminal side of a rabbit gallbladder preparation, a serosa positive transmural p.d. (+2 to +8 mV) arises in a few minutes.Some authors have suggested [16] that the antibiotic would reduce tight-junction selectivity and the negative p.d. due to the backdiffusion of Na+ salts from the lateral spaces: then the opposite positive p.d., created by a hypothetical electrogenic Na+ pump, would be revealed. Against such an explanation, the experiments reported here show that, in parallel with the transepithelial p.d. changes, after the antibiotic addition, the luminal membrane potential is largely depolarized and the ratio between the mucosal and serosal cell resistance decreases. Moreover, the dependence on K+ of the luminal membrane potential is strongly reduced. Ten minutes after the antibiotic addition, modifications of cell water, of cell ion concentrations and contents and of net water transport begin to be observed. Conversely, during the first 10-min period of treatment, no alteration in tight-junction selectivity is detectable by imposing dilution potentials across the tissue; by tracer technique a significant decrease in tight-junction selectivity is observed only 30 min after treatment.Choline substitution for Na+ completely abolishes amphotericin B effects, whereas Cl– replacement by SO
4
2–
does not affect the polyene action. As a conclusion, the primary action of the antibiotic consists of an increase of Na+ conductance at the luminal cell barrier. Only a small fraction of the actual emf variation is measured across the whole epithelium because of the shunt in tight junctions. 相似文献