全文获取类型
收费全文 | 145篇 |
免费 | 9篇 |
出版年
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 6篇 |
2014年 | 4篇 |
2013年 | 10篇 |
2012年 | 11篇 |
2011年 | 8篇 |
2010年 | 3篇 |
2009年 | 4篇 |
2008年 | 2篇 |
2007年 | 4篇 |
2006年 | 9篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 7篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 5篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1989年 | 2篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 3篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1965年 | 2篇 |
1964年 | 3篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1958年 | 1篇 |
1957年 | 1篇 |
1921年 | 1篇 |
排序方式: 共有154条查询结果,搜索用时 15 毫秒
101.
102.
Host-guest study of left-handed polyproline II helix formation. 总被引:4,自引:0,他引:4
M A Kelly B W Chellgren A L Rucker J M Troutman M G Fried A F Miller T P Creamer 《Biochemistry》2001,40(48):14376-14383
103.
Moulton V Gardner PP Pointon RF Creamer LK Jameson GB Penny D 《Journal of molecular evolution》2000,51(4):416-421
Opinion is strongly divided on whether life arose on earth under hot or cold conditions, the hot-start and cold-start scenarios,
respectively. The origin of life close to deep thermal vents appears as the majority opinion among biologists, but there is
considerable biochemical evidence that high temperatures are incompatible with an RNA world. To be functional, RNA has to
fold into a three-dimensional structure. We report both theoretical and experimental results on RNA folding and show that
(as expected) hot conditions strongly reduce RNA folding. The theoretical results come from energy-minimization calculations
of the average extent of folding of RNA, mainly from 0–90°C, for both random sequences and tRNA sequences. The experimental
results are from circular-dichroism measurements of tRNA over a similar range of temperatures. The quantitative agreement
between calculations and experiment is remarkable, even to the shape of the curves indicating the cooperative nature of RNA
folding and unfolding. These results provide additional evidence for a lower temperature stage being necessary in the origin
of life.
Received: 1 March 2000 / Accepted: 14 June 2000 相似文献
104.
The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can adopt this structure. A scale of relative P(II)-helix-forming propensities has been generated but only for single guest residues in a proline-based host system. Here, we present multiple guest residues in a proline-based host system. Using circular dichroism spectroscopy, we have shown that not only single residues, but also short sequences of non-proline residues can adopt the P(II) conformation. 相似文献
105.
106.
Ouabain-insensitive salt and water movements in duck red cells. II. The role of chloride in the volume response
下载免费PDF全文
![点击此处可从《The Journal of general physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
This paper describes the effect of external chloride on the typical swelling response induced in duck red cells by hypertonicity or norepinephrine. Lowering chloride inhibits swelling and produces concomitant changes in net movements of sodium and potassium in ouabain-treated cells, which resemble the effect of lowering external sodium or potassium. Inhibition is the same whether chloride is replaced with gluconate or with an osmotic equivalent of sucrose. Since changes in external chloride also cause predictable changes in cell chloride, pH, and water, these variables were systematically investigated by varying external pH along with chloride. Lowering pH to 6.60 does not abolish the response if external chloride levels are normal, although the cells are initially swollen due to the increased acidity. Cells deliberately preswollen in hypotonic solutions with appropriate ionic composition can also respond to norepinephrine by further swelling. These results rule out initial values of cell water, chloride, and pH as significant variables affecting the response. Initial values of the chloride equilibrium potential do have marked effect on the direction and rate of net water movement. If chloride is lowered by replacement with the permeant anion, acetate, E(Cl) is unchanged and a normal response to norepinephrine, which is inhibited by furosemide, is observed. Increasing internal sodium by the nystatin technique also inhibits the response. A theory is developed which depicts that the cotransport carrier proposed in the previous paper (W.F. Schmidt and T.J. McManus. 1977b. J. Gen. Physiol. 70:81-97) moves in response to the net electrochemical potential difference driving sodium and potassium across the membrane. Predictions of this theory fit the data for both cations and anions. 相似文献
107.
108.
109.
Trevor P. Creamer 《Proteins》1998,33(2):218-226
The left-handed polyproline II helix (PPII) is believed to be the preferred conformation for proline-rich regions of sequence in proteins. Such regions have been postulated to be protein-protein interaction domains. The formation of this structure is studied here using simple Monte Carlo computer simulations employing the hard sphere potential. It is found that polyproline sequences adopt only the PPII structure in the simulations. Non-proline, non-glycine residues inserted as guests into polyproline host peptides are conformationally restricted by the following proline residues and tend to be part of the PPII helix. It is found through insertion of two alanine residues into polyproline that the PPII structure is not propagated through more than one non-proline residue. This finding calls into question the hypothesis that proline-rich regions will preferentially adopt this structure since many such sequences are comprised of less than 50% proline residues. Proteins 33:218–226, 1998. © 1998 Wiley-Liss, Inc. 相似文献
110.
B. Creamer 《BMJ (Clinical research ed.)》1964,2(5421):1373-1375