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排序方式: 共有218条查询结果,搜索用时 15 毫秒
101.
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Pieter Uvin Wouter Everaerts Silvia Pinto Yeranddy A. Alpízar Mathieu Boudes Thomas Gevaert Thomas Voets Bernd Nilius Karel Talavera Dirk De Ridder 《Journal of visualized experiments : JoVE》2012,(66)
The lower urinary tract (LUT) functions as a dynamic reservoir that is able to store urine and to efficiently expel it at a convenient time. While storing urine, however, the bladder is exposed for prolonged periods to waste products. By acting as a tight barrier, the epithelial lining of the LUT, the urothelium, avoids re-absorption of harmful substances. Moreover, noxious chemicals stimulate the bladder''s nociceptive innervation and initiate voiding contractions that expel the bladder''s contents. Interestingly, the bladder''s sensitivity to noxious chemicals has been used successfully in clinical practice, by intravesically infusing the TRPV1 agonist capsaicin to treat neurogenic bladder overactivity1. This underscores the advantage of viewing the bladder as a chemosensory organ and prompts for further clinical research. However, ethical issues severely limit the possibilities to perform, in human subjects, the invasive measurements that are necessary to unravel the molecular bases of LUT clinical pharmacology. A way to overcome this limitation is the use of several animal models2. Here we describe the implementation of cystometry in mice and rats, a technique that allows measuring the intravesical pressure in conditions of controlled bladder perfusion.After laparotomy, a catheter is implanted in the bladder dome and tunneled subcutaneously to the interscapular region. Then the bladder can be filled at a controlled rate, while the urethra is left free for micturition. During the repetitive cycles of filling and voiding, intravesical pressure can be measured via the implanted catheter. As such, the pressure changes can be quantified and analyzed. Moreover, simultaneous measurement of the voided volume allows distinguishing voiding contractions from non-voiding contractions3.Importantly, due to the differences in micturition control between rodents and humans, cystometric measurements in these animals have only limited translational value4. Nevertheless, they are quite instrumental in the study of bladder pathophysiology and pharmacology in experimental pre-clinical settings. Recent research using this technique has revealed the key role of novel molecular players in the mechano- and chemo-sensory properties of the bladder. 相似文献
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Maria-J. Uriz Josep-Maria Gili Covadonga Orejas Alicia-R. Perez-Porro 《Polar Biology》2011,34(2):243-255
Sponges are the dominant invertebrates in many Antarctic communities, where they play a decisive structural function thanks
to their abundance and large sizes. However, current knowledge on Antarctic sponges remains poor even with respect to basic
aspects such as taxonomy. Here, we report on an Antarctic species of the genus Stylocordyla, which has been recorded for a long time under the name of the boreal S. borealis due to spicule and growth habit similarities. A thorough study of dense populations of the only Stylocordyla species known up to now from the eastern zone of the Weddell Sea as well as the re-examination of several specimens (including
the type material) of S. borealis has allowed us to assess the variability of the boreal species and to confirm that the austral species is not S. borealis (Lovén, 1868) but a new species of Stylocordyla, different from the other congeners recorded from southern latitudes. The new species S. chupachups commonly dwells on horizontal or slighted sloped hard bottoms of the continental shelf of Weddell Sea, from 100 m to below
400 m depth, although the densest populations usually occur between 150 and 300 m. It is a pioneer species in areas that have
been scoured by icebergs, and thus its presence may be considered an indicator of recent colonisation. 相似文献
105.
106.
Regina Berjano Fernando Roa Salvador Talavera Marcelo Guerra 《Plant Systematics and Evolution》2009,280(3-4):219-227
Aristolochia is the largest genus of the family Aristolochiaceae and the only one with large chromosome number variation. A combination of fluorochrome banding and in situ hybridization of 5S and 45S rDNA probes was used to evaluate the structural karyotype variability of representatives of two subgenera: Siphisia, which seems to have a single chromosome number (2n = 32), probably derived from an old polyploidization event, and Aristolochia, including the Old World section Diplolobus and the New World Gymnolobus. Based on chromosome morphology and on the degree of diploidization of rDNA sites, A. serpentaria (Siphisia) was identified as an old hexaploid, whereas A. paucinervis (Diplolobus) seemed to be a recent hexaploid (2n = 34). The karyotypes of the five analyzed species of section Gymnolobus were structurally more stable than those from Diplolobus, which varied considerably in the type of heterochromatin, chromosome number, and morphology. These data indicate that fluorochrome banding and rDNA localization may substantially improve the cytotaxonomical analysis of this genus. 相似文献
107.
Phylogeography of the invasive weed Hypochaeris radicata (Asteraceae): from Moroccan origin to worldwide introduced populations 总被引:2,自引:1,他引:1
Ortiz MA Tremetsberger K Terrab A Stuessy TF García-Castaño JL Urtubey E Baeza CM Ruas CF Gibbs PE Talavera S 《Molecular ecology》2008,17(16):3654-3667
In an attempt to delineate the area of origin and migratory expansion of the highly successful invasive weedy species Hypochaeris radicata, we analysed amplified fragment length polymorphisms from samples taken from 44 populations. Population sampling focused on the central and western Mediterranean area, but also included sites from Northern Spain, Western and Central Europe, Southeast Asia and South America. The six primer combinations applied to 213 individuals generated a total of 517 fragments of which 513 (99.2%) were polymorphic. The neighbour-joining tree presented five clusters and these divisions were supported by the results of Bayesian analyses: plants in the Moroccan, Betic Sierras (Southern Spain), and central Mediterranean clusters are all heterocarpic. The north and central Spanish, southwestern Sierra Morena, and Central European, Asian and South American cluster contain both heterocarpic (southwestern Sierra Morena) and homocarpic populations (all other populations). The Doñana cluster includes two homocarpic populations. Analyses of fragment parameters indicate that the oldest populations of H. radicata are located in Morocco and that the species expanded from this area in the Late Quaternary via at least three migratory routes, the earliest of which seems to have been to the southwestern Iberian Peninsula, with subsequent colonizations to the central Mediterranean area and the Betic Sierras. Homocarpic populations originated in the southwestern Iberian Peninsula and subsequently spread across north and central Spain, Central Europe and worldwide, where they became a highly successful weed. 相似文献
108.
Fruit production and arrangement within the raceme were studied in two dioecious populations of Ceratonia siliqua (Leguminosae: Caesalpinioideae), an arboreal species that produces caulogenous racemes (emerging only from the old branches) with numerous flowers. Fruit production per raceme was low and similar between years and populations and even between individuals. During flowering, there were considerable flower losses from predation and lack of pollination. A mean of nine flowers per raceme began the transformation into fruits, of which 77% aborted. The final fruit production per raceme increased significantly following hand pollination, but was always very much lower than the availability of flowers in the raceme. The results suggest that fruit production of each raceme is limited by both availability of resources and a deficient pollination. In racemes setting fruit arrangement follows a definite pattern that remains constant between years and populations: fruit production was significantly higher in the apical zone of the raceme and lower in the basal zone. The pollinators of C. siliqua (flies and wasps) showed a clear preference for beginning their visits at the apex of a raceme. As a result, the pollen load deposited on the stigmas decreased from apex to base of the raceme. In most of the flowers situated in the central and basal zone of the raceme, the number of pollen grains deposited on their stigmas was lower than the number of their ovules. The high number of seeds in developed fruits suggests that the plant selectively aborts flowers that receive a smaller pollen load. The results indicate that the final pattern of fruit arrangement within the raceme is a direct result of pollinator activity. 相似文献
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110.