全文获取类型
收费全文 | 566篇 |
免费 | 50篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2019年 | 11篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 15篇 |
2014年 | 16篇 |
2013年 | 21篇 |
2012年 | 27篇 |
2011年 | 29篇 |
2010年 | 19篇 |
2009年 | 22篇 |
2008年 | 24篇 |
2007年 | 27篇 |
2006年 | 32篇 |
2005年 | 30篇 |
2004年 | 26篇 |
2003年 | 19篇 |
2002年 | 14篇 |
2001年 | 32篇 |
2000年 | 19篇 |
1999年 | 16篇 |
1998年 | 18篇 |
1997年 | 13篇 |
1996年 | 6篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 3篇 |
1992年 | 14篇 |
1991年 | 9篇 |
1990年 | 16篇 |
1989年 | 8篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1979年 | 6篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1971年 | 4篇 |
1970年 | 3篇 |
1969年 | 5篇 |
1968年 | 5篇 |
1967年 | 2篇 |
排序方式: 共有616条查询结果,搜索用时 15 毫秒
141.
Abstract. Terminally differentiated HL-60 cells undergoing programmed cell death (apoptosis) in culture were found to have a disrupted microtubular network. Treatment of undifferentiated HL-60 cells with microtubule-disrupting agents alone was found to induce apoptosis en masse in these cells. In contrast, disruption of microfilaments did not induce apoptosis; instead these cells underwent necrosis, the pathological mode of cell death. Apoptosis in response to microtubule disruption in HL-60 cells was characterized by cell shape changes, nuclear condensation followed by fragmentation and the separation of the cell into numerous intact fragments, termed apoptotic bodies. Apoptosis of these cells was further confirmed by DNA analysis, which demonstrated the activation of an endogenous endonuclease which cleaved the DNA of these cells into oligonucleosomal fragments. Microtubule disrupting agents were found to exert these effects over a wide range of doses. Apoptosis was also inducible in HL-60 cells, in a dose-dependant manner, by the calcium ionophore A23187. Since microtubules are known to be highly sensitive to intracellular calcium fluctuations, this suggests that calcium influx could act at the microtubule level in efTftctino annntnsis 相似文献
142.
Summary Copper is a required trace element for growth of microorganisms since it is a cofactor for numerous enzymes. Also, proteins containing copper are important electron transfer carriers. However, at elevated concentrations, copper can be highly toxic to microorganisms. This review examines copper toxicity and uptake in microorganisms, with an emphasis on copper-resistance mechanisms. 相似文献
143.
144.
145.
146.
147.
148.
149.
Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocytes can themselves be disrupted by using smaller glass beads. 相似文献
150.
Transport of yeast vacuolar trehalase to the vacuole 总被引:4,自引:0,他引:4
We have tested yeast secretory mutants, which define different stages of the secretory pathway, for their levels of vacuolar trehalase activity. Mutations that cause accumulation of secretory proteins in the endoplasmic reticulum or in the Golgi body lead to diminished vacuolar trehalase activity. Mutations that cause accumulation of secretory vesicles have no effect on vacuolar trehalase activity. None of the mutations affects cytoplasmic trehalase activity. These results provide further evidence for the existence of a compartmentalized trehalase in yeast, and demonstrate that the enzyme enters the secretory pathway. 相似文献