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The fine structure of Nosema oryzaephili is very similar to that of the closely related Nosema whitei. The typical number of coils in the polar filament in the spore is slightly different in the two species. The number of coils and their angle of tilt to the long axis of the spore distinguished the two species from many others. 相似文献
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Lysosomal aryl sulphatase in pulmonary alveolar cells 总被引:2,自引:0,他引:2
Summary Lysosomal aryl sulphatase has been localised in the lung at the electron microscopic level using a nitrocatechol sulphate barium chloride medium. Variations in fixative concentration and incubation time were found to be important in minimising lysosomal leakage. The distribution of aryl sulphatase in the lung corresponded closely to that of acid phosphatase. Large amounts were found in alveolar macrophages and small quantities in the type II alveolar epithelial cell. In the latter cell the enzyme was found in the lamellar vacuoles thought to represent the site of surfactant production. The significance of this in regard to the function of these organelles is discussed. 相似文献
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Patrick W. M. Janssen Roger G. Lentle Corrin Hulls Velmurugu Ravindran Ahmed M. Amerah 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2009,179(5):593-604
We studied the caecal contractile activity of the chicken (Gallus gallus) using single caeca that had been cannulated at their proximal and distal ends, and in paired caeca, maintained in situ on
excised segments of gut that were cannulated at the colonic and small intestinal ends. Longitudinal and circular contractile
patterns were characterised using high-definition spatiotemporal mapping. Low amplitude longitudinal contraction waves of
frequency 14.1 cycles/min occurred in the absence of major contractile events. These were termed fast phasic and appeared
to be mediated by slow waves. The nature of major spontaneous contractions occurring in the single caecum varied with the
level of caecal distension. Type A contractions occurred when the caecum was not distended, originated from variable sites
and propagated in both directions. Type B or C contractile events occurred when the caecum was moderately or fully distended,
originated from a predominantly distal site and propagated proximally. On diameter maps, each type B event comprised a succession
of contractions which had similar propagation speeds, frequency and direction to fast phasic contractions. Type C events were
comprised of a succession of higher amplitude contractions with no appreciable propagation. Perfusion of saline via the colon
resulted in fluid entering both caeca and the onset of aborad contractions in their proximal canals. Saline was also seen
to flow between caeca during contractile events however no saline was seen to enter the small intestine as has been postulated
by other workers. 相似文献
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Julia J. Mack Abigail A. Corrin Sergio L. dos Santos e Lucato James C.Y. Dunn Benjamin W. Wu Brian N. Cox 《Biotechnology and bioengineering》2013,110(3):936-946
A novel magnetically actuated scaffold was used to explore the effects of strain stimulus on the proliferation and spatial distribution of smooth muscle cells and improve cell viability in the scaffold interior by pumping nutrients throughout the structure. Magnetically actuable scaffolds were fabricated in a tube shape by winding electrospun sheets of a biodegradable polymer modified with magnetic Fe2O3 nanoparticles. Prior to rolling, the sheets were seeded with smooth muscle cells and wound into tubes with diameter 5.2 mm and wall thickness 0.2 mm. The tubular scaffolds were actuated by a magnetic field to induce a cyclic crimping deformation, which applies strain stimulus to the cells and pumps nutrient fluid through the porous tube walls. Comparison with non‐actuated controls shows that magnetic actuation increases the total cell count throughout the scaffold after 14 days of incubation. Furthermore, whereas cell density as a function of position through the tube wall thickness showed a minimum in the mid‐interior in the controls after 14 days due to cell starvation, the actuated scaffolds displayed a maximum cell density. Comparison of cell distributions with the expected spatial variations in strain amplitude and nutrient flux implies that both strain stimulus and nutrient pumping are significant factors in cell proliferation. Biotechnol. Bioeng. 2013; 110: 936–946. © 2012 Wiley Periodicals, Inc. 相似文献
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Understanding how natural selection drives evolution is a key challenge in evolutionary biology. Most studies of adaptation focus on how a single environmental factor, such as increased temperature, affects evolution within a single species. The biological relevance of these experiments is limited because nature is infinitely more complex. Most species are embedded within communities containing many species that interact with one another and the physical environment. To understand the evolutionary significance of such ecological complexity, experiments must test the evolutionary impact of interactions among multiple species during adaptation. Here we highlight an experiment that manipulates species composition and tracks evolutionary responses within each species, while testing for the mechanisms by which species interact and adapt to their environment. We also discuss limitations of previous studies of adaptive evolution and emphasize how an experimental evolution approach can circumvent such shortcomings. Understanding how community composition acts as a selective force will improve our ability to predict how species adapt to natural and human-induced environmental change. 相似文献
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Evolutionary divergence of platelet-derived growth factor alpha receptor signaling mechanisms
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Hamilton TG Klinghoffer RA Corrin PD Soriano P 《Molecular and cellular biology》2003,23(11):4013-4025
Receptor tyrosine kinases (RTKs) direct diverse cellular and developmental responses by stimulating a relatively small number of overlapping signaling pathways. Specificity may be determined by RTK expression patterns or by differential activation of individual signaling pathways. To address this issue we generated knock-in mice in which the extracellular domain of the mouse platelet-derived growth factor alpha receptor (PDGFalphaR) is fused to the cytosolic domain of Drosophila Torso (alpha(Tor)) or the mouse fibroblast growth factor receptor 1 (alpha(FR)). alpha(Tor) homozygous embryos exhibit significant rescue of neural crest and angiogenesis defects normally found in PDGFalphaR-null embryos yet fail to rescue skeletal or extraembryonic defects. This phenotype was associated with the ability of alpha(Tor) to stimulate the mitogen-activated protein (MAP) kinase pathway to near wild-type levels but failure to completely activate other pathways, such as phosphatidylinositol (PI) 3-kinase. The alpha(FR) chimeric receptor fails to rescue any aspect of the PDGFalphaR-null phenotype. Instead, alpha(FR) expression leads to a gain-of-function phenotype highlighted by ectopic bone development. The alpha(FR) phenotype was associated with a failure to limit MAP kinase signaling and to engage significant PI3-kinase response. These results suggest that precise regulation of divergent downstream signaling pathways is critical for specification of RTK function. 相似文献
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