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981.
Michael J Cwik Maozhi Liang Kelly Deyo Carlotta Andrews James Fischer 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,693(2):1572
A reversed-phase high-performance liquid chromatographic assay for the simultaneous determination of phenytoin and fosphenytoin, a prodrug for phenytoin, in human plasma and plasma ultrafiltrate is described. For plasma, the method involves simple extraction of drugs with diethyl ether and evaporation of solvent, followed by injection of the reconstituted sample onto a reversed-phase C18 column. Plasma ultrafiltrate is injected directly into the HPLC column. Compounds are eluted using an ion-pair mobile phase containing 20% acetonitrile. The eluent is monitored by UV absorbance at 210 nm. The fosphenytoin standard curves are linear in the concentration range 0.4 to 400 μg/ml for plasma and 0.03 to 80 μg/ml for ultrafiltrate. Phenytoin standard curves are linear from 0.08 to 40 μg/ml for plasma and from 0.02 to 5.0 μg/ml for ultrafiltrate. No interferences with the assay procedure were found in drug-free blank plasma or plasma ultrafiltrate. Relative standard deviation for replicate plasma or ultrafiltrate samples was less than 5% at concentrations above the limit of quantitation for both within- and between-run calculations. 相似文献
982.
A. Bresinsky Margit Jarosch M. Fischer Ines Schönberger Birgit Wittmann-Bresinsky 《Plant biology (Stuttgart, Germany)》1999,1(3):327-333
Abstract: This study aimed to test the monophyly and present a generic concept of Paxillus s. I. Of special interest were the Southern hemisphere representatives of this genus. Using the primer combination LR0R-LR5, DNA sequence data were generated from the nuclear encoded 28S gene. A data set containing 900 bases was created for 30 different species. Phylogenetic analysis showed 3 main monophyletic groups, also supported by morphological, anatomical, ecological and chemical data. Taxonomic interpretation of these clearly differentiated groups corresponded to the genera PaxilIus s. str. and Tapinella and lead to the proposal of a new Southern hemisphere genus, Austro-paxillus , mycorrhizal with Nothofagus . New combinations are proposed. 相似文献
983.
Luminita Göbbel Martin S. Fischer Timothy D. Smith John R. Wible Kunwar P. Bhatnagar 《Acta zoologica》2004,85(1):41-52
The vomeronasal organ (VNO) is a chemosensory structure of the nasal septum found in most tetrapods. Although potential behavioural correlates of VNO function have been shown in two of the three elephant species, its morphology in Loxodonta africana has not been studied. The development of the VNO and its associated structures in the African elephant are described in detail using serially sectioned material from fetal stages. The results show that many components of the VNO complex (e.g. neuroepithelium, receptor‐free epithelium, vomeronasal nerve, paravomeronasal ganglia, blood vessels, vomeronasal cartilage) are well developed even in a 154‐day‐old fetus, in which the VNO opens directly into the oral cavity with only a minute duct present. However, the vomeronasal glands and their ducts associated with the VNO were developed only in the 210‐day‐old fetus. Notably, in this fetus, the vomeronasal–nasopalatine duct system had acquired a pathway similar to that described in the adult Asian elephant; the VNOs open into the oral cavity via the large palatal parts of the nasopalatine ducts, which are lined by a stratified squamous epithelium. The paired palatal ducts initially coursed anteriorly at an angle of 45° from the oral recess and/or the oral cavity mucosa, and merged into the vomeronasal duct. This study confirms the unique characteristics of the elephant VNO, such as its large size, the folded epithelium of the VNO tube, and the dorsomedial position of the neuroepithelium. The palatal position and exclusive communication of the VNO with the oral cavity, as well as the partial reduction of the nasopalatine duct, might be related to the unique elephantid craniofacial morphogenesis, especially the enormous growth of the tusk region, and can be seen as autapomorphies. 相似文献
984.
D Engelbrecht Zantut-Wittmann L Persoli M A Tambascia E Fischer D Franco Maldonado A M Costa E Jo?o Pavin 《Hormones et métabolisme》2004,36(7):492-500
Autoimmune chronic lymphocytic thyroiditis appears in two forms, goitrous and atrophic. The evidence available is not enough to prove that the goitrous precedes the atrophic form, but immunogenetic analysis suggests that they may be distinct entities. The distribution of HLA class II alleles DRB1* and DQB1* was verified in patients from the region of Campinas, S?o Paulo, Brazil with both forms of thyroiditis. Ninety-one patients with primary hypothyroidism through autoimmune thyroiditis were classified as goitrous - 54 patients, 42.27 +/- 11.72 years old, having had hypothyroidism for 8.57 +/- 6.63 years - or atrophic - 37 patients, 42.72 +/- 12.01 years old, hypothyroidism for 6.73 +/- 4.07 years. The distribution of class II alleles was determined, DRB1* and DQB1* were genotyped after purifying DNA blood samples using the DNAzol technique, and the low-resolution PCR-SSP system was utilized for determination of generic alleles. Chi-square and Fisher's exact test were utilized to compare the distribution frequency of HLA alleles and the significant p-values were subjected to Bonferroni correction. We have demonstrated that the DRB1*04 allele is associated with autoimmune thyroiditis, and that there are genotypic differences regarding the presentation forms with a strong association between DRB1*04 and DQB1*03 and the atrophic form only. 相似文献
985.
A Fischer 《Cellular and molecular biology, including cyto-enzymology》2001,47(8):1269-1275
Gene therapy is raising incredible hopes. The prospects of treating numbers of severe pathologies (hereditary, cancerous, degenerative or infectious) are vast. Nevertheless, the technological bolts to lift are still numerous, whether they be bringing the vectors into focus, the systems of expression of transgenes or the neutralization of immune responses of the host against the vector, the product of transgenes, or the knowledge of the considered pathologies of physiopathology. Solving these difficulties entails the gathering of multiple disciplines, from chemistry to medicine, passing through virology and immunology. 相似文献
986.
Federico Fischer Debora Raimondi Alessandro Aliverti Giuliana Zanetti 《European journal of biochemistry》2002,269(12):3005-3013
The gene fprA of Mycobacterium tuberculosis, encoding a putative protein with 40% identity to mammalian adrenodoxin reductase, was expressed in Escherichia coli and the protein purified to homogeneity. The 50-kDa protein monomer contained one tightly bound FAD, whose fluorescence was fully quenched. FprA showed a low ferric reductase activity, whereas it was very active as a NAD(P)H diaphorase with dyes. Kinetic parameters were determined and the specificity constant (kcat/Km) for NADPH was two orders of magnitude larger than that of NADH. Enzyme full reduction, under anaerobiosis, could be achieved with a stoichiometric amount of either dithionite or NADH, but not with even large excess of NADPH. In enzyme titration with substoichiometric amounts of NADPH, only charge transfer species (FAD-NADPH and FADH2-NADP+) were formed. At NADPH/FAD ratios higher than one, the neutral FAD semiquinone accumulated, implying that the semiquinone was stabilized by NADPH binding. Stabilization of the one-electron reduced form of the enzyme may be instrumental for the physiological role of this mycobacterial flavoprotein. By several approaches, FprA was shown to be able to interact productively with [2Fe-2S] iron-sulfur proteins, either adrenodoxin or plant ferredoxin. More interestingly, kinetic parameters of the cytochrome c reductase reaction catalyzed by FprA in the presence of a 7Fe ferredoxin purified from M. smegmatis were determined. A Km value of 30 nm and a specificity constant of 110 microM(-1) x s(-1) (10 times greater than that for the 2Fe ferredoxin) were determined for this ferredoxin. The systematic name for FprA is therefore NADPH-ferredoxin oxidoreductase. 相似文献
987.
D Ch Wirtz H Fischer M Neuss J Ammenwerth F U Niethard R Marx 《Biomedizinische Technik》2003,48(6):154-161
The main cause of aseptic loosening of cemented stems in total hip arthroplasty is the hydrolytic degradation of the metal-cement interface. In order to prevent this debonding a new multilayer method of coating the implant surface involving the use of a silica-/silane technique to create a durable adhesive bond between metal stem and bone cement has been developed. The biocompatibility of all the elements of the multilayer system was confirmed using a human osteoblast cell culture test. For sterilization purposes, gamma irradiation with a 25 kGy effective dose proved to be the method of choice. The proven biocompatibility and successful sterilization of the coated implants are the main prerequisites for in-vivo usage. On the technical side, the bonding effectiveness of the multilayer coating system was demonstrated by the tensile test, which revealed a significant improvement in the adhesive strength of the cement-metal bond under prolonged moist conditions similar to those met with in the human body. 相似文献
988.
Alfonso G. Ober Leovigildo Quijano Lowell E. Urbatsch Nikolaus H. Fischer 《Phytochemistry》1984,23(6):1289-1292
Chemical analysis of Calea subcordata yielded three new guaianolides, 8-epi-isobutyrylrupicolin A, 8-epi-isobutyrylrupicolin B and subcordatolide A, which represent the first guaianolide-type sesquiterpene lactones reported in the genus Calea. The structures of the new compounds were established by chemical and spectroscopic methods. 相似文献
989.
Montanoa revealii and M. mollissima yielded a series of germacra-12,6β-olides similar to those previously reported from M. hibiscifolia and M. pteropoda. 相似文献
990.
K. Fischer H. Hölzel K. Kral 《Journal of Zoological Systematics and Evolutionary Research》2006,44(4):285-289
The external morphology of the compound eyes of 13 species of the Ascalaphidae family (Insecta, Neuroptera) from Africa, Asia and Europe was studied in relation to the habitat, phylogeny and time of activity during the day. The six species with undivided eyes (Haplogleniinae) are nocturnal; four inhabit more or less open terrain, while two inhabit more or less dense vegetation. Of the seven species with divided eyes (Ascalaphinae), three are diurnal, one is crepuscular and nocturnal, and three are nocturnal. It was found that two of the diurnal species inhabit open terrain and open forest, and one inhabits dense vegetation; the crepuscular and nocturnal species inhabits open terrain; and two of the nocturnal species inhabit open terrain, while one inhabits dense vegetation. The results are discussed in relation to the hypothesis that divided eyes evolved from undivided eyes, originally serving as an adaptation to daytime vision in open terrain. 相似文献