Individual variation in sleep behaviour in blue tits Cyanistes caeruleus: assortative mating and associations with fitness‐related traits

Sleep is ubiquitous in animals, but there is great inter‐ and intraspecific variation in the daily amount of sleep that is needed. Chronic sleep curtailment or experimental sleep deprivation are known to impair health and performance of individuals, but not much is known about the fitness consequences of naturally occurring variation in sleep behaviour. Here we test for assortative mating in sleep behaviour and for correlations between sleep phenotypes and reproductive success and survival in a free‐living blue tit population. We found that partners of a social breeding pair were mated assortatively in regard to standardized awakening time, i.e. awakening time relative to that of other birds of the same sex in the population. We found no evidence for assortative mating for other sleep parameters. In female blue tits no sleep parameter that we measured was significantly correlated with lay date or clutch size. Females that had extra‐pair young in their brood did not differ in awakening time, or in any other sleep parameter, compared to females without extra‐pair young. In males, the probability of siring extra‐pair young was related to sleep onset and sleep duration, but not as predicted. Males that began to sleep earlier and slept longer were more likely to sire extra‐pair offspring. None of the sleep parameters were significantly correlated with local survival of first‐year birds. Our results suggest that there is no strong effect of variation in sleep behaviour on fitness in blue tits, at least under natural conditions. Such a relationship might only become evident when natural sleep patterns are experimentally disturbed, or when sleep quality is affected by anthropogenic noise or light pollution.     

Effects of crop residues in soil on phymatotrichum root rot of cotton

The effects of cropping pea or barley and the decomposition of their residues on Phymatotrichum root rot of cotton were studied for five years. Little or no changes occurred in pH, content of carbon or moisture in the soil, but there were significant increases in populations of competitive microflora. Best control was obtained in infested soil which was cropped to either pea or barley in winter or in soil which received the higher tonnage of Papago pea plants. Reduction in incidence of root rot may be attributed to premature germination of overwintering propagules making hyphae vulnerable to the chemicals of decomposition and to competition of microflora in the soil.Former Graduate Assistant in Research, Associate Professor, Professor, and Former Professor of Plant Pathology     

Artefacts and collectors in the tropics of North Queensland

This paper outlines some of the ways early artefact collecting contributed to the definition of the Australian region now known and marketed as the ‘World Heritage Wet Tropics’. While others have collected in this region, we focus on the collecting activities of Hermann Klaatsch and the work of Norman Tindale to explore some factors that contributed to their claims that certain artefacts represent a region and its history. We argue that these understandings of region and the past, along with the now widely dispersed artefacts, maintain a lively, albeit transformed, presence in current debates about Aboriginal regional culture, linking assertions of rights to lost and stolen cultural property with notions of large‐scale environmental management within the ‘Wet Tropics’.     

Targeted integration by homologous recombination enables in situ tagging and replacement of genes in the marine microeukaryote Diplonema papillatum

Diplonemids are a group of highly diverse and abundant marine microeukaryotes that belong to the phylum Euglenozoa and form a sister clade to the well-studied, mostly parasitic kinetoplastids. Very little is known about the biology of diplonemids, as few species have been formally described and just one, Diplonema papillatum, has been studied to a decent extent at the molecular level. Following up on our previous results showing stable but random integration of delivered extraneous DNA, we demonstrate here homologous recombination in D. papillatum. Targeting various constructs to the intended position in the nuclear genome was successful when 5′ and 3′ homologous regions longer than 1 kbp were used, achieving N-terminal tagging with mCherry and gene replacement of α- and β-tubulins. For more convenient genetic manipulation, we designed a modular plasmid, pDP002, which bears a protein-A tag and used it to generate and express a C-terminally tagged mitoribosomal protein. Lastly, we developed an improved transformation protocol for broader applicability across laboratories. Our robust methodology allows the replacement, integration as well as endogenous tagging of D. papillatum genes, thus opening the door to functional studies in this species and establishing a basic toolkit for reverse genetics of diplonemids in general.     

Nutrient emission reduction scenarios in the North Sea: An abatement cost and ecosystem integrity analysis

Economic cost–benefit appraisal (and its sub-set cost-effectiveness) of ecosystem conservation and/or pollution abatement strategies have proved to be powerful decision-making aids. But the monetary economic valuation of ecosystem goods and services (gains and losses) can only provide a good indication of social welfare impacts under certain conditions and in selective contexts. The values derived through this appraisal process will, for a number of measures, be underestimates of the full total system value [Turner, R.K., Paavola, J., Cooper, P. Farber, S., Jessamy, V., Georgiou, S., 2003. Valuing nature: lessons learned and future research directions. Ecol. Econ. 46, 493–510]. The economic analysis is best suited to assessing the value of ‘marginal’ gains and losses in ecosystem goods/services and not the total destruction of whole systems (including life support systems, the value of which is not commensurate with monetary values and/or is infinitely high). In this study economic costs and what we call ‘ecological risk’ analysis are used to appraise the implementation costs and ecological benefits of selected measures for combating eutrophication. Ecological risk is expressed in terms of ecosystem integrity and resilience. The paper presents three regional case studies dealing with the issue of nutrient emission reduction to the southern North Sea, namely the catchments/estuaries of the Humber (UK), the Rhine (Germany and The Netherlands) and the Elbe (Czech Republic and Germany). On the basis of these comparative regional examples, wider implications in the light of international management of the North Sea are presented.A range of nutrient reduction scenarios have been deployed within the overall OSPAR target agreement of 50% nitrogen and phosphorous reduction compared with 1985 levels. Each scenario assumes pollution reduction measures, characterised in terms of their overall implementation costs and nutrient-reduction effects. Specific policy instruments analysed were: the creation of more intertidal habitat via managed coastal realignment in the Humber area, farm-based land cover changes in the Rhine catchment and a mix of agricultural regime and wastewater treatment plant (WWTP) improvements in the Elbe area. The ecological consequences associated with each reduction scenario have been modelled [using the ERSEM model, see Baretta, J.W., Ebenhöh, W., Ruardij, P., 1995. An overview over the European Regional Sea Ecosystem Model, a complex marine ecosystem model. Neth. J. Sea Res. 33 (3/4), 233–246] for the coastal zones supplied from the rivers Elbe, Humber and Rhine. The modelled ecological quality indicators, which describe scenario effects on the coastal zone ecosystem, are then aggregated in terms of ecosystem integrity and ecological risk. The results are presented in terms of two selected key-indicators: implementation costs of the abatement measures and changes in ecological risk status, across the different catchments and assuming different scenarios. They thus provide a possible basis for international agreement negotiations at the North Sea scale.     

Mechanism of mate detection in parasitoid wasps: sound and vibratory cues change with the developmental progress of future mates inside host pupal cases

Insects including parasitoid wasps use acoustic and vibratory signals in the context of sexual communication, mate recognition, courtship and mating. Males of the parasitoid wasp Pimpla disparis Viereck (Hymenoptera: Ichneumonidae) detect insect host pupae parasitized by a conspecific female, learn their location, visit them repeatedly and remain on or near them when the prospective mate nears emergence. In the present study, the acoustic and vibratory cues that males exploit to detect the presence and track the developmental progress of a future mate inside a host pupal case are investigated. Responses are acquired from developing parasitoids (DePa) by airborne sound and laser Doppler vibrometer recordings, after gently stimulating each of 20 wax moth host pupae with a paintbrush on days 1–23 post parasitism. Sound and vibratory cues produced by DePa are detectable from day 7 onward and relate mostly to spinning movements. Parameters of sound and vibratory cues (amplitude, dominant frequency, upper limit of frequency band) change significantly over time and thus could ‘inform’ a visiting adult male about the stage of development of DePa. Adult males antennating a parasitized pupa and flying around it also induce vibrations, which in turn may inform DePa about the presence of a male. There is no experimental evidence for true signalling and rapid information exchange between DePa and adult males. Delaying reply signals may help DePa avoid attacks by illicit receivers of such signals, including female (hyper)parasitoids and invertebrate predators.