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71.
Cytosolic protein binding to band-3 protein inhibits endocytosis of isolated human erythrocyte membranes.
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Recent studies of haemoglobin binding to the cytoplasmic side of the erythrocyte membrane have shown that the predominant high-affinity interaction occurs with the major integral membrane protein known as band-3 protein and that this interaction may occur within the intact erythrocyte in a manner regulated by cell pH. We report here that haemoglobin and glyceraldehyde 3-phosphate dehydrogenase binding to band-3 protein in isolated membranes can inhibit endocytosis during vesiculation in vitro. The specificity of this effect was demonstrated by showing that myoglobin, which has an affinity for the membrane fully one to two orders of magnitude lower than that for haemoglobin, does not inhibit endocytosis. 相似文献
72.
The O(R) regions from several lambdoid bacteriophages contain the three regulatory sites O(R)1, O(R)2 and O(R)3, to which the Cro and CI proteins can bind. These sites show imperfect dyad symmetry, have similar sequences, and generally lie on the same face of the DNA double helix. We have developed a computational method, which analyzes the O(R) regions of additional phages and predicts the location of these three sites. After tuning the method to predict known O(R) sites accurately, we used it to predict unknown sites, and ultimately compiled a database of 32 known and predicted O(R) binding site sets. We then identified sequences of the recognition helices (RH) for the cognate Cro proteins through manual inspection of multiple sequence alignments. Comparison of Cro RH and consensus O(R) half-site sequences revealed strong one-to-one correlations between two amino acids at each of three RH positions and two bases at each of three half-site positions (H1-->2, H3-->5 and H6-->6). In each of these three cases, one of the two amino acid/base-pairings corresponds to a contact observed in the crystal structure of a lambda Cro/consensus operator complex. The alternate amino acid/base combinations were rationalized using structural models. We suggest that the pairs of amino acid residues act as binary switches that efficiently modulate specificity for different consensus half-site variants during evolution. The observation of structurally reasonable amino acid-to-base correlations suggests that Cro proteins share some common rules of recognition despite their functional and structural diversity. 相似文献
73.
Nup153 affects entry of messenger and ribosomal ribonucleoproteins into the nuclear basket during export
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Soop T Ivarsson B Björkroth B Fomproix N Masich S Cordes VC Daneholt B 《Molecular biology of the cell》2005,16(12):5610-5620
A specific messenger ribonucleoprotein (RNP) particle, Balbiani ring (BR) granules in the dipteran Chironomus tentans, can be visualized during passage through the nuclear pore complex (NPC). We have now examined the transport through the nuclear basket preceding the actual translocation through the NPC. The basket consists of eight fibrils anchored to the NPC core by nucleoprotein Nup153. On nuclear injection of anti-Nup153, the transport of BR granules is blocked. Many granules are retained on top of the nuclear basket, whereas no granules are seen in transit through NPC. Interestingly, the effect of Nup153 seems distant from the antibody-binding site at the base of the basket. We conclude that the entry into the basket is a two-step process: an mRMP first binds to the tip of the basket fibrils and only then is it transferred into the basket by a Nup153-dependent process. It is indicated that ribosomal subunits follow a similar pathway. 相似文献
74.
Effects of ENU dosage on mouse strains 总被引:15,自引:0,他引:15
Monica J. Justice Don A. Carpenter Jack Favor Angelika Neuhauser-Klaus Martin Hrabé de Angelis Dian Soewarto Amy Moser Sabine Cordes Darla Miller Verne Chapman John S. Weber Eugene M. Rinchik Patricia R. Hunsicker William L. Russell Vernon C. Bode 《Mammalian genome》2000,11(7):484-488
The germline supermutagen, N-ethyl-N-nitrosourea (ENU), has a variety of effects on mice. ENU is a toxin and carcinogen as well as a mutagen, and strains differ
in their susceptibility to its effects. Therefore, it is necessary to determine an appropriate mutagenic, non-toxic dose of
ENU for strains that are to be used in experiments. In order to provide some guidance, we have compiled data from a number
of laboratories that have exposed male mice from inbred and non-inbred strains or their F1 hybrids to ENU. The results show that most F1 hybrid animals tolerate ENU well, but that inbred strains of mice vary in their longevity and in their ability to recover
fertility after treatment with ENU.
Received: 11 February 2000 / Accepted: 11 February 2000 相似文献
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