全文获取类型
收费全文 | 5479篇 |
免费 | 695篇 |
国内免费 | 3篇 |
专业分类
6177篇 |
出版年
2021年 | 54篇 |
2019年 | 55篇 |
2018年 | 63篇 |
2017年 | 57篇 |
2016年 | 76篇 |
2015年 | 172篇 |
2014年 | 162篇 |
2013年 | 213篇 |
2012年 | 248篇 |
2011年 | 233篇 |
2010年 | 156篇 |
2009年 | 158篇 |
2008年 | 184篇 |
2007年 | 198篇 |
2006年 | 205篇 |
2005年 | 165篇 |
2004年 | 178篇 |
2003年 | 170篇 |
2002年 | 200篇 |
2001年 | 165篇 |
2000年 | 198篇 |
1999年 | 147篇 |
1998年 | 73篇 |
1997年 | 75篇 |
1996年 | 51篇 |
1995年 | 55篇 |
1994年 | 68篇 |
1993年 | 58篇 |
1992年 | 129篇 |
1991年 | 99篇 |
1990年 | 117篇 |
1989年 | 123篇 |
1988年 | 128篇 |
1987年 | 114篇 |
1986年 | 91篇 |
1985年 | 97篇 |
1984年 | 97篇 |
1983年 | 81篇 |
1982年 | 86篇 |
1981年 | 54篇 |
1979年 | 81篇 |
1978年 | 75篇 |
1977年 | 54篇 |
1976年 | 55篇 |
1975年 | 55篇 |
1974年 | 68篇 |
1973年 | 61篇 |
1972年 | 56篇 |
1970年 | 50篇 |
1968年 | 49篇 |
排序方式: 共有6177条查询结果,搜索用时 15 毫秒
91.
Abstract In newly invaded communities, interspecific competition is thought to play an important role in determining the success of the invader and its impact on the native community. In southern Australia, the native Polistes humilis was the predominant social wasp prior to the arrival of the exotic Vespula germanica (Hymenoptera: Vespidae). Both species forage for similar resources (water, pulp, carbohydrate and protein prey), and concerns have arisen about potential competition between them. The aim of this study was to identify the protein foods that these wasps feed on. As many prey items are masticated by these wasps to the degree that they cannot be identified using conventional means, morphological identification was complemented by sequencing fragments of the mitochondrial 16S rRNA gene. GenBank searches using blast and phylogenetic analyses were used to identify prey items to at least order level. The results were used to construct complete prey inventories for the two species. These indicate that while P. humilis is restricted to feeding on lepidopteran larvae, V. germanica collects a variety of prey of invertebrate and vertebrate origin. Calculated values of prey overlap between the two species are used to discuss the implications of V. germanica impacting on P. humilis. Results obtained are compared to those gained by solely 'conventional' methods, and the advantages of using DNA-based taxonomy in ecological studies are emphasized. 相似文献
92.
Cooper Matthew A. Bernstein Irwin S. Fragaszy Dorothy M. de Waal Frans B. M. 《International journal of primatology》2001,22(4):663-683
We examined how aggressive, affiliative, and sexual behavior function to integrate male capuchins (Cebus apella) into a new social group. Nine males were exchanged among four social groups. We performed instantaneous scans and all-occurrence sampling during baseline, introduction, and follow-up periods. The study included three different introduction situations: 1) males familiar to one another were introduced to a group with no other adult male, 2) males unfamiliar to one another were introduced to a group with no other adult male, and 3) males familiar to one another were introduced to a group with an existing elderly, resident male. Severe aggression occurred in situations 2 and 3, but the introductions were peaceful in situation 1. In all cases proceptive females were among the first individuals to affiliate with the males, and males did not appear to compete for access to proceptive females. Following their period of proceptivity, the females that had cycled remained preferred social partners for the males. Immature animals also quickly affiliated with the new males, and the males tolerated the attention from immatures. Affiliative relationships between the males and nonproceptive females developed slowly, and while male-female aggression was mild, aggression among adult males (familiar and unfamiliar) had the potential to be severe. 相似文献
93.
Urea transport in Saccharomyces cerevisiae occurs by two pathways. The first mode of uptake is via an active transport system which: (i) has an apparent Km value of 14 muM, (ii) is absolutely dependent upon energy metabolism, (iii) requires pre-growth of the cultures in the presence of oxaluric acid, gratuitous inducer of the allantoin degradative enzymes, and (iv) is sensitive to nitrogen repression. The second mode of uptake which occurs at external urea concentrations in excess of 0.5 mM is via either passive or facilitated diffusion. 相似文献
94.
95.
Manon Grube Sukhbinder Kumar Freya E. Cooper Stuart Turton Timothy D. Griffiths 《Proceedings. Biological sciences / The Royal Society》2012,279(1746):4496-4504
This work tests the relationship between auditory and phonological skill in a non-selected cohort of 238 school students (age 11) with the specific hypothesis that sound-sequence analysis would be more relevant to phonological skill than the analysis of basic, single sounds. Auditory processing was assessed across the domains of pitch, time and timbre; a combination of six standard tests of literacy and language ability was used to assess phonological skill. A significant correlation between general auditory and phonological skill was demonstrated, plus a significant, specific correlation between measures of phonological skill and the auditory analysis of short sequences in pitch and time. The data support a limited but significant link between auditory and phonological ability with a specific role for sound-sequence analysis, and provide a possible new focus for auditory training strategies to aid language development in early adolescence. 相似文献
96.
Is the virulence of parasites an outcome of optimized infection? Virulence has often been considered an inevitable consequence of parasite reproduction when the cost incurred by the parasite in reducing the fitness of its current host is offset by increased infection of new hosts. More recent models have focused on how competition occurring between parasites during co-infection might effect selection of virulence. For example, if co-infection was common, parasites with higher intrinsic growth rates might be selected, even at the expense of being optimally adapted to infect new hosts. If growth rate is positively correlated with virulence, then competition would select increased virulence. We tested these models using a plasmid-encoded virulence determinant. The virulence determinant did not contribute to the plasmid's reproduction within or between hosts. Despite this, virulent plasmids were more successful than avirulent derivatives during selection in an environment allowing within-host competition. To explain these findings we propose and test a model in which virulent parasites are selected by reducing the reproduction of competitors. 相似文献
97.
98.
Christopher S. Bland Eric T. Wang Anthony Vu Marjorie P. David John C. Castle Jason M. Johnson Christopher B. Burge Thomas A. Cooper 《Nucleic acids research》2010,38(21):7651-7664
Recent genome-wide analyses have elucidated the extent of alternative splicing (AS) in mammals, often focusing on comparisons of splice isoforms between differentiated tissues. However, regulated splicing changes are likely to be important in biological transitions such as cellular differentiation, or response to environmental stimuli. To assess the extent and significance of AS in myogenesis, we used splicing-sensitive microarray analysis of differentiating C2C12 myoblasts. We identified 95 AS events that undergo robust splicing transitions during C2C12 differentiation. More than half of the splicing transitions are conserved during differentiation of avian myoblasts, suggesting the products and timing of transitions are functionally significant. The majority of splicing transitions during C2C12 differentiation fall into four temporal patterns and were dependent on the myogenic program, suggesting that they are integral components of myogenic differentiation. Computational analyses revealed enrichment of many sequence motifs within the upstream and downstream intronic regions near the alternatively spliced regions corresponding to binding sites of splicing regulators. Western analyses demonstrated that several splicing regulators undergo dynamic changes in nuclear abundance during differentiation. These findings show that within a developmental context, AS is a highly regulated and conserved process, suggesting a major role for AS regulation in myogenic differentiation. 相似文献
99.
100.
Tettamanti G Grimaldi A Ferrarese R Palazzi M Perletti G Valvassori R Cooper EL Lanzavecchia G de Eguileor M 《Tissue & cell》2003,35(3):199-212
The aim of the present work is to describe histologically, histochemically and immunocytochemically, the sequence of events that lead to first and second set rejection of allo- or xenograft in leeches. Graft responses of leeches are comparable and are described following specific steps: inflammatory phase, rejection phase and granulation tissue formation (including re-epithelialisation, angiogenesis and fibroplasia).The responses to first and second graft in first set graft rejection as well as to the first transplant in second set graft experiments are identical and in the time span of a week all grafts are destroyed and disappear. In the second set graft rejection experiments the responses against the second transplant are markedly accelerated. The second graft shows massive structural alterations and it is rapidly rejected, within 3-4 days.Our results permit to highlight that in leeches there is a specific responsiveness of immune system similar to those described in highly divergent phyla. 相似文献