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251.
Silke Uhrlaß Peter Mayser Roman Schwarz Daniela Koch Constanze Krüger Irene Korfmann Pietro Nenoff 《Mycopathologia》2018,183(2):391-398
Nannizzia praecox, formerly known as Microsporum praecox, is a geophilic dermatophyte. Up to now 31 cases of human tinea have been reported in the literature, most of them with an inflammatory course. Three recent cases diagnosed in Germany within 1 year suggest that the fungus might be a more common cause of human dermatophytosis than reported so far. This might be based on the fact that N. praecox is often found in an equine environment and that horse riding is becoming more popular recently. 相似文献
252.
Wook Joon Chung Stefan Kindler Constanze Seidenbecher Craig C. Garner 《Journal of neurochemistry》1996,66(3):1273-1281
Abstract: MAP2, a dendritically localized microtubule-associated protein (MAP), consists of a pair of high molecular mass (280 kDa) polypeptides, MAP2a and MAP2b, and several low molecular mass (70 kDa) proteins called MAP2c. Although MAP2b and MAP2c have been shown to arise via alternative splicing, it was not clear whether MAP2a is also created by alternative splicing or by posttranslational modification. Using epitope peptide mapping, we have demonstrated that an element specific to MAP2a is situated at its N-terminal end. A cDNA clone from an adult rat brain library was found to contain an additional 246 nucleotides situated at the 5' end of the 9-kb MAP2 mRNA. Antibodies generated against the encoded protein sequence recognize specifically MAP2a in rat brain homogenates. Moreover, although MAP2a, like MAP2b, is found in dendrites and cell bodies, its temporal appearance and cell type-specific distribution in rat brain differs from MAP2b. 相似文献
253.
Inducible chromatin priming is associated with the establishment of immunological memory in T cells
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254.
Michael Koch Constanze Breithaupt StefanIlka GerhardtHaase Stefan Weber Mark Cushman Robert Huber Adelbert Bacher Markus Fischer 《European journal of biochemistry》2004,271(15):3208-3214
The amino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeast Schizosaccharomyces pombe was replaced by tyrosine. The structures of the W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, were determined by X-ray crystallography at resolutions of 2.7-2.8 A. Whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin. These findings provide an explanation for the absence of the long wavelength shift in optical absorption spectra of riboflavin bound to the mutant enzyme. The structures of the mutants are important tools for the interpretation of the unusual physical properties of riboflavin in complex with lumazine synthase. 相似文献
255.
Though observations on re-colonisation of post-fire sites in the Mediterranean Basin are plentiful, there still is an ongoing
debate on the interrelation of fire regimes and species traits related to fire adaptation. Most of the studies found are restricted
to particular species or claim to present community attributes. Therefore they often lack information for the evaluation of
evolutionary evidence and historical contingency of the local fire regime and other abiotic conditions, which may act as selective
pressure for plant regeneration strategies. Indeed, knowledge about the success of regeneration mechanisms and their interrelation
with ecological factors is essential for the interpretation of the high spatio-temporal variability found in post-fire species
performance. Such knowledge would be necessary to assess the potential of different regeneration mechanisms to cope with ongoing
land-use and climate change—a crucial scientific challenge.
A summary is given of the knowledge about the limits and potential of plant regeneration mechanisms after fire in the Mediterranean
Basin, along with corresponding studies conducted in other parts of the world with similar climatic conditions in order to
present the fullest possible picture. Moreover, the positive or negative impacts of particular parameters of a fire regime
on different regeneration strategies (post-fire seeders, resprouters, and facultative resprouters) are explained and discussed
in the light of published literature. To conclude, reference is made to scientific gaps that need to be filled in order to
analyse species resistance and community resilience absorbing possible climate or land use changes. 相似文献
256.
The Pu.1 locus is differentially regulated at the level of chromatin structure and noncoding transcription by alternate mechanisms at distinct developmental stages of hematopoiesis
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257.
Complex bird clocks. 总被引:8,自引:0,他引:8
E Gwinner R Brandst?tter 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2001,356(1415):1801-1810
The circadian pacemaking system of birds comprises three major components: (i) the pineal gland, which rhythmically synthesizes and secretes melatonin; (ii) a hypothalamic region, possibly equivalent to the mammalian suprachiasmatic nuclei; and (iii) the retinae of the eyes. These components jointly interact, stabilize and amplify each other to produce a highly self-sustained circadian output. Their relative contribution to overt rhythmicity appears to differ between species and the system may change its properties even within an individual depending, for example, on its state in the annual cycle or its photic environment. Changes in pacemaker properties are partly mediated by changes in certain features of the pineal melatonin rhythm. It is proposed that this variability is functionally important, for instance, for enabling high-Arctic birds to retain synchronized circadian rhythms during the low-amplitude zeitgeber conditions in midsummer or for allowing birds to adjust quickly their circadian system to changing environmental conditions during migratory seasons. The pineal melatonin rhythm, apart from being involved in generating the avian pacemaking oscillation, is also capable of retaining day length information after isolation from the animal. Hence, it appears to participate in photoperiodic after-effects. Our results suggest that complex circadian clocks have evolved to help birds cope with complex environments. 相似文献
258.
259.
Target Structures of the CD8+-T-Cell Response to Human Cytomegalovirus: the 72-Kilodalton Major Immediate-Early Protein Revisited 总被引:3,自引:0,他引:3
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Florian Kern Ingolf Pascal Surel Nicole Faulhaber Claudia Frmmel Jens Schneider-Mergener Constanze Schnemann Petra Reinke Hans-Dieter Volk 《Journal of virology》1999,73(10):8179-8184
Cell-mediated immunity plays an essential role in the control of infection with the human cytomegalovirus (HCMV). However, only a few CD8(+)-T-cell epitopes are known, with the majority being contained in the pp65 phosphoprotein, which is believed to dominate the CD8(+)-T-cell response to HCMV. Here, we have readdressed the issue of CD8(+) T cells specific for the 72-kDa major immediate-early protein (IE-1), which is nonstructural but is found very early and throughout the replicative cycle. Using a novel flow-cytometric assay, we were able to identify CD8(+)-T-cell epitopes (by IE-1 peptide-specific induction of cytokine synthesis) and simultaneously measure the frequency of cells directed against them. For this purpose, 81 pentadecamer peptides covering the complete 491-amino-acid sequence of IE-1 were tested on peripheral blood mononuclear cells of anti-HCMV immunoglobulin G-seropositive donors. At least 10 new epitopes were identified, and the fine specificity and presenting HLA molecule of the first of them was determined. The frequencies of CD8(+) T cells directed against IE-1 were similar to those directed against pp65 in donors tested with known pp65-derived peptides. Importantly, additional testing of a corresponding set of peptides covering the complete sequence of pp65 on 10 of these donors identified individuals whose CD8(+) T cells recognized IE-1 but not pp65 and vice versa, clearly illustrating that either protein may be a major target. In summary, our results suggest that IE-1 is far more important as a CD8(+)-T-cell target than current opinion suggests. 相似文献
260.
Merijn A G de Bakker Wessel van der Vos Kaylah de Jager Wing Yu Chung Donald A Fowler Esther Dondorp Stephan N F Spiekman Keng Yih Chew Bing Xie Rafael Jimnez Constanze Bickelmann Shigeru Kuratani Radim Blazek Peter Kondrashov Marilyn B Renfree Michael K Richardson 《Molecular biology and evolution》2021,38(10):4222