Diet of nestling Linnets (Acanthis cannabina L.)

Summary The diet of nestling linnets (Acanthis cannabina) living on a heathland in Brittany (France) was studied by faecal pellet analysis during the 1982 and 1983 breeding seasons. Seeds composed the main part of the diet. According to month,Taraxacum officinale (Compositae),Stellaria media (Caryophyllaceae),Brassica napus (Cruciferae),Anthoxantum odoratum (Gramineae) andEuphorbia helioscopia (Euphorbiaceae) dominated. Nestling age and brood size influenced parents' food selection, especially seed size selection. The younger the nestlings, the larger the selected seeds. Increased food demands of larger broods were compensated by larger seeds selected. Adults generally fed seeds within given size limits, but these limits were not imperative. Adults were opportunistic, raising their broods with the most available seeds, such as rape (Brassica napus) seed, but at the same time provisioning them with weed seeds from fallow lands or meadows.

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Zur Nahrung nestjunger Hänflinge (Acanthis cannabina L.)

Zusammenfassung Die Nahrung nestjunger Hänflinge in einem Heidegebiet der Bretagne (Frankreich) in den Jahren 1982 und 1983 bestand vornehmlich aus Sämereien. Je nach Monat dominiertenTaraxacum officinale (Compositae),Stellaria media (Caryophyllaceae),Brassica napus (Cruciferae),Anthoxantum odoratum (Gramineae) undEuphorbia helioscopia (Euphorbiaceae). Die von den Elternvögeln verfütterte Nahrung und ihre Größe variierte mit dem Alter der Nestlinge und der Brutgröße. Jüngere Nestlinge wurden mit größerer Sämereien gefüttert als ältere. Der höhere Nahrungsbedarf großer Bruten wurde durch die Wahl größerer Objekte kompensiert. Die Nahrungswahl der Elternvögel war opportunistisch nach den jeweils am zahlreichsten verfügbaren Sämereien, z. B. Raps (Brassica napus). Die Nahrungssuche erfolgte sowohl im Brachland wie auf Wiesen.

     

Interaction of α-lactalbumin with dimyristoylphosphatidylcholine vesicles. III. Influence of the temperature and of the lipid-to-protein molar ratio on the complex formation

We investigated the interaction between α-lactalbumin and sonicated dimyristoylphosphatidylcholine at pH 4 and different temperatures. (1) At 23°C and lipid-to-protein molar ratios below 170, the interaction results in a disruption of the original vesicles to form smaller complex particles. By the sedimentation velocity method we determined for this particle a molar mass of (1.05 ± 0.16) · 10⁶ g·mol^?1. The lipid-to-protein molar ratio within the complex particle is 70/1, as earlier estimated. It follows that there are approximately 1200 lipid and 17 α-lactalbumin molecules per particle. At molar ratios above 170, α-lactalbumin strongly associates with the vesicles. In this case the vesicle entity remains. The ability of α-lactalbumin to break up the vesicles at this temperature is determined by the number of protein molecules which are required in the complex particle. (2) By means of fluorescence polarization of the lipophilic probe 1,6-diphenyl-1,3,5-hexatriene and energy transfer of the tryptophan groups of the protein to 1,3-(1,1′-dipyrenyl)propane located in the hydrocarbon region of the vesicles, it is shown that with increasing temperature above 25°C, complexes of decreasing internal lipid-to-protein molar ratio are formed. However, by electron microscopy we show that the overall size of these complexes remains approximately the same, i.e., bars with dimensions $\text{70 × 220}\text{A}\text{?}$. A temperature-reversible transformation occurs between these complexes, which cannot be isolated by gel chromatography. In contrast, the complex of molar ratio 70/1 remains stable at lower temperatures.     

Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex

Mycobacterium tuberculosis and Mycobacterium leprae, the two main mycobacterial pathogens in humans, produce highly specific long chain beta-diols, the dimycocerosates of phthiocerol, and structurally related phenolic glycolipid (PGL) antigens, which are important virulence factors. In addition, M. tuberculosis also secretes glycosylated p-hydroxybenzoic acid methyl esters (p-HBAD) that contain the same carbohydrate moiety as the species-specific PGL of M. tuberculosis (PGL-tb). The genes involved in the biosynthesis of these compounds in M. tuberculosis are grouped on a 70-kilobase chromosomal fragment containing three genes encoding putative glycosyltransferases: Rv2957, Rv2958c, and Rv2962c. To determine the functions of these genes, three recombinant M. tuberculosis strains, in which these genes were individually inactivated, were constructed and biochemically characterized. Our results demonstrated that (i) the biosynthesis of PGL-tb and p-HBAD involves common enzymatic steps, (ii) the Rv2957, Rv2958c, and Rv2962c genes are involved in the formation of the glycosyl moiety of the two classes of molecules, and (iii) the product of Rv2962c catalyzes the transfer of a rhamnosyl residue onto p-hydroxybenzoic acid ethyl ester or phenolphthiocerol dimycocerosates, whereas the products of Rv2958c and Rv2957 add a second rhamnosyl unit and a fucosyl residue to form the species-specific triglycosyl appendage of PGL-tb and p-HBAD. The recombinant strains produced provide the tools to study the role of the carbohydrate domain of PGL-tb and p-HBAD in M. tuberculosis pathogenesis.     

Molar enamel thickness in the chacma baboon, Papio ursinus (Kerr 1792)

Modern humans exhibit increasing relative enamel thickness from M1 to M3. Some biomechanical (basic lever) models predict that the more distal molars in humans encounter higher occlusal forces, and it has been postulated that this provides a functional explanation for the observed gradient in relative enamel thickness. However, constrained three-dimensional models and experimental observations suggest that there is a reduction in bite force potential from M1 to M3, which would be consistent with the tendency for humans to reduce the size of the distal molars. In this regard, it has been postulated that the distal increase in enamel thickness is a consequence of crown size reduction; thus, it is unnecessary to invoke functional scenarios to explain this phenomenon. We assess these competing proposals by examining relative enamel thickness in a catarrhine primate (Papio ursinus) that exhibits crown size increase from M1 to M3. The molar row of P. ursinus is positioned relatively far forward of the temporomandibular joint, which results in the baboon being able to exert relatively greater muscle forces during posterior biting in comparison to modern humans. Thus, a significant distalward gradient of increasing enamel thickness would be expected in P. ursinus according to the hypothesis that posits it to be functionally related to bite force. The present study reveals no significant difference in relative enamel thickness along the molar row in P. ursinus. This finding lends support to the notion that the relatively thicker enamel of human distal molars is related primarily to their reduction in size. This carries potential implications for the interpretation of enamel thickness in phylogenetic reconstructions: the relatively thick molar enamel shared by modern humans and some of our fossil relatives may not be strictly homologous, in that it may result from different underlying developmental mechanisms.     

Glycosylation of Rapana thomasiana hemocyanin. Comparison with other prosobranch (gastropod) hemocyanins

The carbohydrate content and composition of hemocyanins (Hcs) of three prosobranchs (gastropods), Rapana thomasiana, Megathura crenulata and Haliotis tuberculata, were compared. The analyses were performed by gas-liquid chromatography after methanolysis, re-N-acetylation and trimethylsilylation. The two structural subunits of R. thomasiana Hc, RtH1 and RtH2, both showed 2.6% (w/w) carbohydrate content with very similar monosaccharide composition, indicative for N-glycosylation. The two isoforms of M. crenulata Hc (KLH), KLH1 and KLH2, on the other hand, definitely differed in glycosylation: KLH2 (3.4% carbohydrate, w/w) comprised relatively less mannose and more N-acetylgalactosamine than KLH1 (3.0% carbohydrate, w/w), in agreement with the fact that O-glycosylation has been observed in a functional unit (FU) of KLH2. For the Hc of the abalone H. tuberculata, with 4.5% (w/w) carbohydrate, appreciable amounts of 3-O-methyl-d-mannose and 3-O-methyl-d-galactose were detected, showing that the occurrence of methylated sugars is not restricted to the Hcs of pulmonates. From the structural subunit RtH2 of Rapana Hc the FUs RtH2-b and RtH2-d were isolated. On the basis of amino acid sequence analysis and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of the respective native and PNGase-F-treated glycopeptides, one N-glycosylation site was found for each FU. This site was located at Asn-405 for RtH2-b and at Asn-394 for RtH2-d; the carbohydrate moiety corresponded to GlcNAc2Man6 and GlcNAc2Man5, respectively. A comparison was made with the N-glycosylation sites of other FUs of Rapana Hc.     

In vitro antioxidant properties,free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica

Background

Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC.

Results

This study demonstrated that M. myristica has scavenging properties against DPPH^•, OH^•, NO^•, and ABTS^• radicals which vary in a dose depending manner. It also showed an antioxidant potential that was comparable with that of Butylated Hydroxytoluene (BHT) and vitamin C used as standard. The aqueous ethanol extract of M. myristica barks (AEH); showed a significantly higher content in polyphenolic compounds (21.44 ± 0.24 mg caffeic acid/g dried extract) and flavonoid (5.69 ± 0.07 quercetin equivalent mg/g of dried weight) as compared to the other studied extracts. The HPLC analysis of the barks and leaves revealed the presence of several polyphenols. The acids (3,4-OH-benzoic, caffeic, gallic, O- and P- coumaric, syringic, vanillic), alcohols (tyrosol and OH-tyrosol), theobromine, quercetin, rutin, catechine and apigenin were the identified and quantified polyphenols. All the tested extracts demonstrated a high protective potential on the superoxide dismutase (SOD), catalase and peroxidase activities.

Conclusion

Finally, the different extracts from M. myristica and specifically the aqueous ethanol extract reveal several properties such as higher free radical scavenging properties, significant antioxidant capacities and protective potential effects on liver enzymes.     

The effect of BTX compounds on the biodegradation of ETBE by an ETBE degrading bacterial consortium

Ethyl tert-butyl ether (ETBE) is a fuel oxygenate that is commonly used in Europe to achieve complete combustion of automobile fuels and to control air pollution. It is potentially toxic and can enter the human system via contaminated water bodies. In the present study, we have identified an enriched bacterial consortium from a gasoline-contaminated site that can degrade ETBE. Bacterial consortium A was able to degrade 47% of the added ETBE in 4 days and it continued to degrade up to 51% in 9 days. Consortium A consisted of Xanthomonas sp., Methylibium sp., Methylobacillus sp., and Methylovorus sp. which were identified as the participating bacteria during ETBE degradation by DGGE — 16S rDNA analysis. In addition to ETBE, this consortium degraded benzene, toluene and xylene isomers (BTX) when they were present as the sole carbon source. The degradation efficiency increased predominantly when ETBE was included as an additional carbon source. Interestingly, the degradation of ETBE decreased to 14% in 9 days when present with BTX compounds. We report that ETBE degradation is slowed down or inhibited when BTX compounds are present. This is a crucial observation for ETBE degradation in the natural environment.     

Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus

Vector population control using insecticides is a key element of current strategies to prevent malaria transmission in Africa. The introduction of effective insecticides, such as the organophosphate pirimiphos-methyl, is essential to overcome the recurrent emergence of resistance driven by the highly diverse Anopheles genomes. Here, we use a population genomic approach to investigate the basis of pirimiphos-methyl resistance in the major malaria vectors Anopheles gambiae and A. coluzzii. A combination of copy number variation and a single non-synonymous substitution in the acetylcholinesterase gene, Ace1, provides the key resistance diagnostic in an A. coluzzii population from Côte d’Ivoire that we used for sequence-based association mapping, with replication in other West African populations. The Ace1 substitution and duplications occur on a unique resistance haplotype that evolved in A. gambiae and introgressed into A. coluzzii, and is now common in West Africa primarily due to selection imposed by other organophosphate or carbamate insecticides. Our findings highlight the predictive value of this complex resistance haplotype for phenotypic resistance and clarify its evolutionary history, providing tools to for molecular surveillance of the current and future effectiveness of pirimiphos-methyl based interventions.     

Strong, long-term temporal dynamics of an ecological network

Nature is organized into complex, dynamical networks of species and their interactions, which may influence diversity and stability. However, network research is, generally, short-term and depict ecological networks as static structures only, devoid of any dynamics. This hampers our understanding of how nature responds to larger disturbances such as changes in climate. In order to remedy this we studied the long-term (12-yrs) dynamics of a flower-visitation network, consisting of flower-visiting butterflies and their nectar plants. Global network properties, i.e. numbers of species and links, as well as connectance, were temporally stable, whereas most species and links showed a strong temporal dynamics. However, species of butterflies and plants varied bimodally in their temporal persistance: Sporadic species, being present only 1-2(-5) years, and stable species, being present (9-)11-12 years, dominated the networks. Temporal persistence and linkage level of species, i.e. number of links to other species, made up two groups of species: Specialists with a highly variable temporal persistence, and temporally stable species with a highly variable linkage level. Turnover of links of specialists was driven by species turnover, whereas turnover of links among generalists took place through rewiring, i.e. by reshuffling existing interactions. However, in spite of this strong internal dynamics of species and links the network appeared overall stable. If this global stability-local instability phenomenon is general, it is a most astonishing feature of ecological networks.