全文获取类型
收费全文 | 807篇 |
免费 | 68篇 |
出版年
2022年 | 6篇 |
2021年 | 11篇 |
2020年 | 4篇 |
2019年 | 10篇 |
2018年 | 10篇 |
2017年 | 9篇 |
2016年 | 24篇 |
2015年 | 45篇 |
2014年 | 31篇 |
2013年 | 42篇 |
2012年 | 48篇 |
2011年 | 69篇 |
2010年 | 38篇 |
2009年 | 34篇 |
2008年 | 37篇 |
2007年 | 49篇 |
2006年 | 35篇 |
2005年 | 33篇 |
2004年 | 29篇 |
2003年 | 28篇 |
2002年 | 34篇 |
2001年 | 15篇 |
1999年 | 8篇 |
1998年 | 12篇 |
1997年 | 14篇 |
1996年 | 12篇 |
1995年 | 6篇 |
1994年 | 10篇 |
1993年 | 5篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 11篇 |
1973年 | 4篇 |
1972年 | 5篇 |
1970年 | 4篇 |
1966年 | 4篇 |
1965年 | 4篇 |
1964年 | 7篇 |
1963年 | 5篇 |
排序方式: 共有875条查询结果,搜索用时 15 毫秒
71.
Higham JP Brent LJ Dubuc C Accamando AK Engelhardt A Gerald MS Heistermann M Stevens M 《Behavioral ecology》2010,21(4):739-746
Animal coloration has provided many classical examples of both natural and sexual selection. Methods to study color signals range from human assessment to models of receiver vision, with objective measurements commonly involving spectrometry or digital photography. However, signal assessment by a receiver is not objective but linked to receiver perception. Here, we use standardized digital photographs of female rhesus macaque (Macaca mulatta) face and hindquarter regions, combined with estimates of the timing of the female fertile phase, to assess how color varies with respect to this timing. We compare objective color measures (camera sensor responses) with models of rhesus vision (retinal receptor stimulation and visual discriminability). Due to differences in spectral separation between camera sensors and rhesus receptors, camera measures overestimated color variation and underestimated luminance variation compared with rhesus macaques. Consequently, objective digital camera measurements can produce statistically significant relationships that are probably undetectable to rhesus macaques, and hence biologically irrelevant, while missing variation in the measure that may be relevant. Discrimination modeling provided results that were most meaningful (as they were directly related to receiver perception) and were easiest to relate to underlying physiology. Further, this gave new insight into the function of such signals, revealing perceptually salient signal luminance changes outside of the fertile phase that could potentially enhance paternity confusion. Our study demonstrates how, even for species with similar visual systems to humans, models of vision may provide more accurate and meaningful information on the form and function of visual signals than objective color measures do. 相似文献
72.
Joseph Karem Stephen A. Woods Francis Drummond Constance Stubbs 《Biocontrol Science and Technology》2010,20(3):257-274
This was the first study to have surveyed the spatial and temporal structure of Apocrita wasps in lowbush blueberry fields, a unique native agricultural landscape in Maine and eastern Canada. The relative abundances of wasps associated with lowbush blueberry (Vaccinium angustifolium Ait.) were investigated in 33 blueberry fields throughout Washington County, Maine, USA. Native wasps were captured during the springs and summers of 1997 and 1998 in Malaise traps erected along a transect in each field. Vegetation sampling was also conducted along these transects to quantify available floral resources. Data indicate the abundance of the total wasp community was positively associated with the abundance of sheep laurel (Kalmia angustifolia L.). Relationships between trap capture of 13 wasp morphospecies and other flowering weeds were also investigated. Most taxa in 1998 were positively associated with one or more of the following flowering plants: bunchberry (Cornus canadensis L.), bush honeysuckle (Diervilla lonicera P. Mill.), dogbane (Apocynum androsaemifolium L.), sheep laurel, and witherod (Viburnum nudum var. cassinoides L.). Similar results were not evident in 1997 because the method used to sample vegetation was not as extensive as that used in 1998. However, sheep laurel was positively associated with the wasp genera Microplitis spp. and Phanerotoma spp. during both years. 相似文献
73.
Vanya P. Bogoeva Antonio Varriale Constance M. John Sabato D'Auria 《Proteomics》2010,10(10):1946-1953
Human galectin‐3 (hGal‐3) is a mammalian lectin involved in regulation of RNA splicing, apoptosis, cell differentiation, and proliferation. Multimerized extracellular hGal‐3 is thought to crosslink cells by binding to glycoproteins and glycosylated cancer antigens on the cell surface or extracellular matrix. Fluorescence spectroscopy and circular dichroism were used to study the interaction of hGal‐3 with two anticancer agents: bohemine and Zn porphyrin (ZnTPPS4). The dissociation constant (kD) for binding of bohemine with hGal‐3 was kD 0.23±0.05 μM. The hyperbolic titration curve indicated the presence of a single bohemine binding site. The binding of ZnTPPS4 to hGal‐3 (with and without lactose) is of high affinity having kD=0.18–0.20 μM and is not inhibited by lactose, indicating that ZnTPPS4 and carbohydrate bind different sites. Circular dichroism spectra of the hGal‐3 complexes suggested that the binding of the hydrophobic compounds changed the hGal‐3 secondary structure. In summary, we show that two compounds with anticancer activity, bohemine and ZnTPPS4, have high affinity for hGal‐3 at a site that is distinct from its carbohydrate site. Since hGal‐3 binds to several carbohydrate cancer antigens, the results suggest that it may have utility in the targeted delivery of drugs for cancer. 相似文献
74.
Séverine Vuilleumier Benjamin M. Bolker Olivier Lévêque 《Theoretical population biology》2010,78(3):225-45
Ocean currents, prevailing winds, and the hierarchical structures of river networks are known to create asymmetries in re-colonization between habitat patches. The impacts of such asymmetries on metapopulation persistence are seldom considered, especially rarely in theoretical studies. Considering three classical models (the island, the stepping stone and the distance-dependent model), we explore how metapopulation persistence is affected by (i) asymmetry in dispersal strength, in which the colonization rate between two patches differs in direction, and (ii) asymmetry in connectivity, in which the overall colonization pattern displays asymmetry (circulating or dendritic networks). Viability can be drastically reduced when directional bias in dispersal strength is higher than 25%. Re-colonization patterns that allow for strong local connectivity provide the highest persistence compared to systems that allow circulation. Finally, asymmetry has relatively weak effects when metapopulations maintain strong general connectivity. 相似文献
75.
76.
77.
78.
Signal transduction and cell‐type specific regulation of matrix metalloproteinase gene expression: Can MMPs be good for you? 总被引:3,自引:0,他引:3
An abundance of literature over the past several years indicates a growing interest in the role of matrix metalloproteinases (MMPs) in normal physiology and in disease pathology. MMPs were originally defined by their ability to degrade the extracellular matrix, but it is now well documented that their substrates extend far beyond matrix components. Recent reviews discuss the structure and function of the MMP family members, as well as the promoter sequences that control gene expression. Thus, we focus on the signal transduction pathways that confer differential cell-type expression of MMPs, as well as on some novel non-matrix degrading functions of MMPs, particularly their intracellular location where they may contribute to apoptosis. In addition, increasing data implicate MMPs as "good guys", protective agents in some cancers and in helping to resolve acute pathologic conditions. Despite the intricate and complicated roles of MMPs in physiology and pathology, the goal of designing therapeutics that can selectively target MMPs remains a major focus. Developing MMP inhibitors with targeted specificity will be difficult; success will depend on understanding the role of these enzymes in homeostasis and on the careful delineation of mechanisms by which this family of enzymes mediates disease pathology. 相似文献
79.
The role of Stn1p in Saccharomyces cerevisiae telomere capping can be separated from its interaction with Cdc13p
下载免费PDF全文
![点击此处可从《Genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The function of telomeres is twofold: to facilitate complete chromosome replication and to protect chromosome ends against fusions and illegitimate recombination. In the budding yeast Saccharomyces cerevisiae, interactions among Cdc13p, Stn1p, and Ten1p are thought to be critical for promoting these processes. We have identified distinct Stn1p domains that mediate interaction with either Ten1p or Cdc13p, allowing analysis of whether the interaction between Cdc13p and Stn1p is indeed essential for telomere capping or length regulation. Consistent with the model that the Stn1p essential function is to promote telomere end protection through Cdc13p, stn1 alleles that truncate the C-terminal 123 residues fail to interact with Cdc13p and do not support viability when expressed at endogenous levels. Remarkably, more extensive deletions that remove an additional 185 C-terminal residues from Stn1p now allow cell growth at endogenous expression levels. The viability of these stn1-t alleles improves with increasing expression level, indicating that increased stn1-t dosage can compensate for the loss of Cdc13p-Stn1p interaction. However, telomere length is misregulated at all expression levels. Thus, an amino-terminal region of Stn1p is sufficient for its essential function, while a central region of Stn1p either negatively regulates the STN1 essential function or destabilizes the mutant Stn1 protein. 相似文献
80.
Molecular and immunological evaluation of the expression of cancer/testis gene products in human colorectal cancer 总被引:1,自引:0,他引:1
Alves PM Lévy N Bouzourene H Viatte S Bricard G Ayyoub M Vuilleumier H Givel JC Halkic N Speiser DE Romero P Lévy F 《Cancer immunology, immunotherapy : CII》2007,56(6):839-847
Tumor-specific gene products, such as cancer/testis (CT) antigens, constitute promising targets for the development of T cell
vaccines. Whereas CT antigens are frequently expressed in melanoma, their expression in colorectal cancers (CRC) remains poorly
characterized. Here, we have studied the expression of the CT antigens MAGE-A3, MAGE-A4, MAGE-A10, NY-ESO-1 and SSX2 in CRC
because of the presence of well-described HLA-A2-restricted epitopes in their sequences. Our analyses of 41 primary CRC and
14 metastatic liver lesions confirmed the low frequency of expression of these CT antigens. No increased expression frequencies
were observed in metastatic tumors compared to primary tumors. Histological analyses of CRC samples revealed heterogeneous
expression of individual CT antigens. Finally, evidence of a naturally acquired CT antigen-specific CD8+ T cell response could be demonstrated. These results show that the expression of CT antigens in a subset of CRC patients
induces readily detectable T cell responses. 相似文献