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Igwe David Okeh Ihearahu Onyinye Constance Osano Anne Adhiambo Acquaah George Ude George Nkem 《Plant Molecular Biology Reporter》2021,39(4):801-820
Plant Molecular Biology Reporter - Sixty-six accessions of Musa genus with different genomic groups that consisted of wild relatives and cultivated lines were obtained from the International... 相似文献
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M. J. Pettenati Andrew J. Sweatt Patrick Lantz Constance A. Stanton James Reynolds P. Nagesh Rao Richard M. Davis 《Human genetics》1997,101(1):26-29
Structurally and functionally, the human cornea is a highly specialized tissue. The corneal stromal collagen matrix is uniquely
transparent and yet maintains a mechanically tough and chemically impermeable barrier between the eye and environment. We
report for the first time that stromal keratocytes of the human cornea show cytogenetic abnormalities with a frequency that
is unprecedented among normal tissues. The abnormalities are acquired, clonal and nonclonal, primarily aneuploid in nature,
and present in normal as well as diseased corneas.
Received: 10 February 1997 / Accepted: 21 May 1997 相似文献
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Regulated Membrane Localization of Tiam1, Mediated by the NH2-terminal Pleckstrin Homology Domain, Is Required for Rac-dependent Membrane Ruffling and C-Jun NH2-terminal Kinase Activation 总被引:10,自引:0,他引:10
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Andréa N Moreno Gabriela Pereira-da-Silva Constance Oliver Maria Célia Jamur Ademilson Panunto-Castelo Maria Cristina Roque-Barreira 《The journal of histochemistry and cytochemistry》2005,53(6):715-723
The macrophage-derived neutrophil chemotactic factor (MNCF) is a D-galactose-binding lectin that induces neutrophil migration in vitro and in vivo. Neutrophil recruitment induced by MNCF is resistant to glucocorticoid treatment and is inhibited by the lectin-specific sugar, D-galactose. In the present study, we characterized the binding of MNCF to neutrophils and the responses triggered by this binding. Exposure to MNCF resulted in cell polarization, formation of a lamellipodium, and deep ruffles on the cell surface. By confocal microscopy, we observed that MNCF was evenly distributed on the cell surface after 30 min of incubation. The labeling intensity progressively diminished with longer incubations. Internalization kinetics showed that MNCF/ligand complexes were rapidly internalized, reaching maximum intracellular concentrations at 120 min and then decreased thereafter. The binding and internalization of MNCF were selectively inhibited by D-galactose. MNCF-induced neutrophil chemotaxis was inhibited by pertussis toxin. This fact strongly suggests that the MNCF-ligand on the neutrophil surface is a G-protein-coupled receptor (GPCR), similar to receptors for well-established neutrophil attractants. Our observations on the ability of MNCF to activate neutrophils are consistent with the increasing evidence for the participation of animal lectins in the innate immune response. 相似文献
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Presterilized mictotiter plates (96 wells) with BHK-21 cells on 5-mm cover slips were successfully used for cell culture isolation of trachoma from 15 infected conjunctival scrapings. 相似文献