Sample size for testing differences in proportions for the paired-sample design

Miettinen (1968, Biometrics 24, 339-352) presented an approximation for power and sample size for testing the differences between proportions in the matched-pair case. Duffy (1984, Biometrics 40, 1005-1015) gave the exact power for this case and showed that Miettinen's approximation tends to slightly overestimate the power or underestimate the sample size necessary for the design power. A simple alternative approximation that is more conservative is presented here. In many cases, the sample size for the independent-sample case provides a conservative approximation for the matched-pair design.     

Hydro-sedimentology of the Johnstone River estuary

This paper examines the physical consequences of increased catchment sediment yields on the sediment budget and the hydrodynamic setting of the South Johnstone River estuary in North Queensland. A combined study involving hydrological monitoring, assessment of sediment sources, estimation of riverine sediment budget and hydro-sedimentological numerical modelling for estuarine sediment transport is currently underway. Initial field and laboratory observations indicate that the sediment delivery from highly erosion-prone sugar cane cultivations in the tropical catchment has increased dramatically during the last 10 years. This has subsequently given rise to elevated flood levels in both the lower and upper catchment areas, as well as significant modifications to the river bed morphology.     

Siblings with chromosome mosaicism,microcephaly, and growth retardation: the phenotypic expression of a human mitotic mutant?

Summary We report male and female siblings with extreme microcephaly and mental retardation, growth retardation, and multiple chromosome mosaicism. Mental retardation associated with chromosome mosaicism does not always carry a low recurrence risk.     

Capacity of siderophore — producing alkalophilic bacteria to accumulate iron,gallium and aluminum

Summary The ability of alkalophilic bacteria to remove iron, gallium and aluminium from culture media is reported. Of six bacterial strains grown in the presence of iron, gallium or aluminium (10 M), five were able to accumulate iron or gallium, but only two depleted the aluminium stock. A comparison of gallium removal under low (< 1 gmM) or high (10 gmM) iron conditions showed that two isolates accumulated gallium only under low-Fe conditions. One isolate, a coryneform bacterium, was able to grow in the presence of 1, 10 and 100 mg gallium/l, but growth and siderophore production were affected at high gallium concentration. Similar concentrations of gallium were accumulated from cultures initially containing 1 or 100 mg gallium/l. Offsprint requests to: D. J. Gascoyne     

Increased Acetylcholine Synthesis and Release in Brains of Cats with GM1 Gangliosidosis

Cholinergic processes were measured in motor cortex, hippocampus, and striatum of cats in the terminal stages of GM1 gangliosidosis and compared to those of control cats. The greatest difference observed was elevation in the rate of K+-stimulated release of acetylcholine (ACh) from brain slices prepared from affected cats. The K+-stimulated release of endogenous ACh was increased by 31-43% and of newly synthesized ACh by 19-80% in brain slices from different brain regions. All regions that were examined were affected but the greatest effects occurred in cortex. The rate of synthesis of ACh was elevated in cortical and hippocampal slices. Choline acetyltransferase activity in brain regions of cats with GM1 gangliosidosis was not significantly different from that in controls, whereas high-affinity choline transport in cortical synaptosomes was elevated. Muscarinic receptor binding sites were reduced in the cortex, hippocampus, and striatum of GM1 mutant cats, whereas the apparent affinity was not altered. These results indicate that there are major alterations of cholinergic function in the brains of cats with GM1 gangliosidosis.     

Photochemical reactions in interstellar grains photolysis of co,NH3, and H2O

A simulation of the organic layer accreted onto interstellar dust particles was prepared by slow deposition of a CO:NH₃:H₂O gas mixture on an Al block at 10K, with concomitant irradiation with vacuum UV. The residues were analyzed by GC-MS, HPLC, and near IR; a reaction pathway leading from NH₃ to complex alcohol, fatty acid, and amide products in 27 stages is postulated. The astronomical relevance and significance of the observations are discussed.     

A reliable method for detecting the intracellular accumulation of fermentation products: Application to intracellular ethanol analysis

Summary Intracellular ethanol concentrations inSaccharomyces cerevisiae were measured using a high cell density fermentation technique which permits samples to be fixed for analysis in less than two seconds. No accumulation of intracellular ethanol was detected, regardless of the stage of fermentation. The technique eliminates artifacts associated with concentrating the cell sample and provides a reliable means for detecting whether other fermentation products accumulate intracellularly.