全文获取类型
收费全文 | 574篇 |
免费 | 70篇 |
出版年
2022年 | 6篇 |
2021年 | 6篇 |
2019年 | 7篇 |
2018年 | 10篇 |
2017年 | 13篇 |
2016年 | 19篇 |
2015年 | 33篇 |
2014年 | 32篇 |
2013年 | 30篇 |
2012年 | 29篇 |
2011年 | 37篇 |
2010年 | 23篇 |
2009年 | 17篇 |
2008年 | 24篇 |
2007年 | 23篇 |
2006年 | 21篇 |
2005年 | 23篇 |
2004年 | 17篇 |
2003年 | 13篇 |
2002年 | 34篇 |
2001年 | 24篇 |
2000年 | 16篇 |
1999年 | 13篇 |
1998年 | 14篇 |
1997年 | 7篇 |
1996年 | 12篇 |
1995年 | 8篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 11篇 |
1991年 | 8篇 |
1990年 | 3篇 |
1989年 | 11篇 |
1988年 | 12篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 8篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 4篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1967年 | 2篇 |
1965年 | 2篇 |
1934年 | 1篇 |
排序方式: 共有644条查询结果,搜索用时 31 毫秒
21.
Occurrence of 16SrIV Subgroup A Phytoplasmas in Roystonea regia and Acrocomia mexicana Palms with Lethal Yellowing‐like Syndromes in Yucatán,Mexico 下载免费PDF全文
María Narvaez Iván Córdova‐Lara Celso Reyes‐Martínez Carlos Puch‐Hau Luis Mota‐Narvaez Ana Collí Goretti Caamal Nigel Harrison Luis Sáenz Carlos Oropeza 《Journal of Phytopathology》2016,164(11-12):1111-1115
The lethal yellowing (LY) disease and LY‐type syndromes affecting several palm species are associated with 16SrIV phytoplasmas in the Americas. In Mexico, palms of the species Roystonea regia and the native Acrocomia mexicana were found to exhibit LY‐type symptoms, including leaf decay, starting with mature leaves, necrosis and atrophy of inflorescences. DNA extracts obtained from these palms could be amplified by nested‐PCR using phytoplasma‐universal primer pair P1/P7 followed by LY‐group‐specific primer pair LY16Sr/LY16Sf. Blast analysis of the sequences obtained revealed an identity of 100% for R. regia and 99.27% for A. mexicana with 16SrIV‐A strain associated with LY in Florida, USA (Acc. AF498309 ). Computer‐simulated RFLP analysis showed that the patterns for the phytoplasma DNA of the two palm species were highly similar to that for 16SrIV subgroup A strain. A neighbour‐joining tree was constructed, and the sequences of the two palm species clustered in the same clade of group 16SrIV subgroup A. The results therefore support that LY‐type syndromes observed in palms of R. regia and A. mexicana in the Yucatan region of Mexico are associated with 16SrIV subgroup A phytoplasmas. 相似文献
22.
23.
Manzano Carolina Melchert Nicolás A. Coll Araoz Maria V. Virla Eduardo G. Luft Albarracin Erica 《BioControl》2022,67(1):27-37
BioControl - Cosmocomoidea annulicornis (Ogloblin) (Hymenoptera: Mymaridae) is a solitary egg parasitoid of the sharpshooter Tapajosa rubromarginata (Signoret) (Hemiptera: Cicadellidae), vector of... 相似文献
24.
Hernandez-Trejo A B Estrada-Drouaillet JA López-Santillán C Rios-Velasco SE Varela-Fuentes R Rodríguez-Herrera E Osorio-Hernández 《Phyton》2019,88(1):47-54
The control of Spodoptera frugiperda is based
on synthetic insecticides, so some alternatives are the use of
entomopathogenic fungi (EF) and neem extract. The objective of
the study was to evaluate in vitro effectiveness of native EF and
neem extracts on S. frugiperda larvae. Six EF were identified by
DNA sequencing of ITS regions from three EF (Fusarium solani,
Metarrhizium robertsii, Nigrospora spherica and Penicillium
citrinum). They were evaluated in concentrations of 1 × 10⁸ spores/
mL. In addition, a second bioassay was carried out evaluating
only F. solani, M. robertsii and N. sphaerica and the addition
of vegetable oil. On the other hand, extraction of secondary
metabolites from neem seed (Azadirachta indica) was carried
out by performing, mass (g) and solvent volume (mL ethanol
and water) combinations, which were subjected to microwaves
and ultrasound. Subsequently, these extracts were evaluated
in concentrations of 3%, 4% and 5%. A survival analysis was
performed for each of the bioassays. With respect to the results
of the first bioassay, F. solani obtained a probability of survival of
0.476 on the seventh day, while in the second bioassay, M. robertsii
obtained 0.488 survival probability. This suggests that the expected
percentage of larvae that stay alive on the sixth day is 48.8%.
However, in the evaluation of the neem extract the combination
1:12/70% to 4% caused 84% mortality of larvae. The use of native
HE and neem extracts has potential for the control of S. frugiperda. 相似文献
25.
Wu Z Fraley ME Bilodeau MT Kaufman ML Tasber ES Balitza AE Hartman GD Coll KE Rickert K Shipman J Shi B Sepp-Lorenzino L Thomas KA 《Bioorganic & medicinal chemistry letters》2004,14(4):909-912
3,7-Diarylsubstituted imidazopyridines were designed and developed as a new class of KDR kinase inhibitors. A variety of imidazopyridines were synthesized and potent inhibitors of KDR kinase activity were identified with good aqueous solubility. 相似文献
26.
27.
Transfection of epithelioma papulosum cyprini (EPC) carp cells 总被引:1,自引:0,他引:1
The variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) with the genes for -galactosidase from E. coli, for luciferases from firefly or renilla, for the G protein of viral haemorrhagic septicemia virus or for green fluorescent protein under the cytomegalovirus, the SV40 or the T7 polymerase promoters have been studied. Fugene was selected among 10 transfection different reagents because it is simpler to use and it induced maximum efficiences of transfection of 37% equivalent to 10–15 ng -galactosidase per 500000 EPC cells. 相似文献
28.
Eichman BF Ortiz-Lombardía M Aymamí J Coll M Ho PS 《Journal of molecular biology》2002,320(5):1037-1051
Holliday junctions are four-stranded DNA complexes that are formed during recombination and related DNA repair events. Much work has focused on the overall structure and properties of four-way junctions in solution, but we are just now beginning to understand these complexes at the atomic level. The crystal structures of two all-DNA Holliday junctions have been determined recently from the sequences d(CCGGGACCGG) and d(CCGGTACCGG). A detailed comparison of the two structures helps to distinguish distortions of the DNA conformation that are inherent to the cross-overs of the junctions in this crystal system from those that are consequences of the mismatched dG.dA base-pair in the d(CCGGGACCGG) structure. This analysis shows that the junction itself perturbs the sequence-dependent conformational features of the B-DNA duplexes and the associated patterns of hydration in the major and minor grooves only minimally. This supports the idea that a DNA four-way junction can be assembled at relatively low energetic cost. Both structures show a concerted rotation of the adjacent duplex arms relative to B-DNA, and this is discussed in terms of the conserved interactions between the duplexes at the junctions and further down the helical arms. The interactions distant from the strand cross-overs of the junction appear to be significant in defining its macroscopic properties, including the angle relating the stacked duplexes across the junction. 相似文献
29.
30.
Paris R Morales A Coll O Sánchez-Reyes A García-Ruiz C Fernández-Checa JC 《The Journal of biological chemistry》2002,277(51):49870-49876
Ganglioside GD3 (GD3) has emerged as a modulator of cell death pathways due to its ability to interact with mitochondria and disable survival pathways. Because NF-kappaB activation contributes to cancer therapy resistance, this study was undertaken to test whether GD3 modulates the response of human hepatoblastoma HepG2 cells to radio- and chemotherapy. NF-kappaB was activated in HepG2 cells shortly after therapeutic doses of ionizing radiation or daunorubicin treatment that translated into up-regulation of kappaB-dependent genes. These effects were accompanied by minimal killing of HepG2 cells by either ionizing radiation or daunorubicin. However, GD3 pretreatment blocked the nuclear translocation of active kappaB members, without effect on Akt phosphorylation, induced by either treatment. The suppression of kappaB-dependent gene induction by GD3 was accompanied by enhanced apoptotic cell death caused by these therapies. Furthermore, the combination of GD3 plus ionizing radiation stimulated the formation of reactive species followed by the mitochondrial release of cytochrome c and Smac/Diablo and caspase 3 activation. Pretreatment with cyclosporin A before radiotherapy protected HepG2 cells from the therapeutic combination of GD3 plus ionizing radiation. These findings underscore a key role of mitochondria in the response of tumor cells to cancer therapy and highlight the potential relevance of GD3 to overcome resistance to cancer therapy by combining its dual action as a mitochondria-interacting and NF-kappaB-inactivating agent. 相似文献