Modular Skeletal Evolution in Sticklebacks Is Controlled by Additive and Clustered Quantitative Trait Loci

Understanding the genetic architecture of evolutionary change remains a long-standing goal in biology. In vertebrates, skeletal evolution has contributed greatly to adaptation in body form and function in response to changing ecological variables like diet and predation. Here we use genome-wide linkage mapping in threespine stickleback fish to investigate the genetic architecture of evolved changes in many armor and trophic traits. We identify >100 quantitative trait loci (QTL) controlling the pattern of serially repeating skeletal elements, including gill rakers, teeth, branchial bones, jaws, median fin spines, and vertebrae. We use this large collection of QTL to address long-standing questions about the anatomical specificity, genetic dominance, and genomic clustering of loci controlling skeletal differences in evolving populations. We find that most QTL (76%) that influence serially repeating skeletal elements have anatomically regional effects. In addition, most QTL (71%) have at least partially additive effects, regardless of whether the QTL controls evolved loss or gain of skeletal elements. Finally, many QTL with high LOD scores cluster on chromosomes 4, 20, and 21. These results identify a modular system that can control highly specific aspects of skeletal form. Because of the general additivity and genomic clustering of major QTL, concerted changes in both protective armor and trophic traits may occur when sticklebacks inherit either marine or freshwater alleles at linked or possible “supergene” regions of the stickleback genome. Further study of these regions will help identify the molecular basis of both modular and coordinated changes in the vertebrate skeleton.     

Isolation and Characterization of Desulfovibrio dechloracetivorans sp. nov., a Marine Dechlorinating Bacterium Growing by Coupling the Oxidation of Acetate to the Reductive Dechlorination of 2-Chlorophenol

Strain SF3, a gram-negative, anaerobic, motile, short curved rod that grows by coupling the reductive dechlorination of 2-chlorophenol (2-CP) to the oxidation of acetate, was isolated from San Francisco Bay sediment. Strain SF3 grew at concentrations of NaCl ranging from 0.16 to 2.5%, but concentrations of KCl above 0.32% inhibited growth. The isolate used acetate, fumarate, lactate, propionate, pyruvate, alanine, and ethanol as electron donors for growth coupled to reductive dechlorination. Among the halogenated aromatic compounds tested, only the ortho position of chlorophenols was reductively dechlorinated, and additional chlorines at other positions blocked ortho dechlorination. Sulfate, sulfite, thiosulfate, and nitrate were also used as electron acceptors for growth. The optimal temperature for growth was 30°C, and no growth or dechlorination activity was observed at 37°C. Growth by reductive dechlorination was revealed by a growth yield of about 1 g of protein per mol of 2-CP dechlorinated, and about 2.7 g of protein per mole of 2,6-dichlorophenol dechlorinated. The physiological features and 16S ribosomal DNA sequence suggest that the organism is a novel species of the genus Desulfovibrio and which we have designated Desulfovibrio dechloracetivorans. The unusual physiological feature of this strain is that it uses acetate as an electron donor and carbon source for growth with 2-CP but not with sulfate.     

Effects of Transcranial Direct Current Stimulation on the Control of Finger Force during Dexterous Manipulation in Healthy Older Adults

The contribution of poor finger force control to age-related decline in manual dexterity is above and beyond ubiquitous behavioral slowing. Altered control of the finger forces can impart unwanted torque on the object affecting its orientation, thus impairing manual performance. Anodal transcranial direct current stimulation (tDCS) over primary motor cortex (M1) has been shown to improve the performance speed on manual tasks in older adults. However, the effects of anodal tDCS over M1 on the finger force control during object manipulation in older adults remain to be fully explored. Here we determined the effects of anodal tDCS over M1 on the control of grip force in older adults while they manipulated an object with an uncertain mechanical property. Eight healthy older adults were instructed to grip and lift an object whose contact surfaces were unexpectedly made more or less slippery across trials using acetate and sandpaper surfaces, respectively. Subjects performed this task before and after receiving anodal or sham tDCS over M1 on two separate sessions using a cross-over design. We found that older adults used significantly lower grip force following anodal tDCS compared to sham tDCS. Friction measured at the finger-object interface remained invariant after anodal and sham tDCS. These findings suggest that anodal tDCS over M1 improved the control of grip force during object manipulation in healthy older adults. Although the cortical networks for representing objects and manipulative actions are complex, the reduction in grip force following anodal tDCS over M1 might be due to a cortical excitation yielding improved processing of object-specific sensory information and its integration with the motor commands for production of manipulative forces. Our findings indicate that tDCS has a potential to improve the control of finger force during dexterous manipulation in older adults.     

Defending the nest: variation in the alarm aggression response and nest mound damage in the harvester ant Pogonomyrmex occidentalis

A number of studies have found that ant colonies vary in many colony-level phenotypes, including the level of aggression towards non-nestmates. The extent of a colony’s aggression and defense of the nest in response to attacks by predators is likely to affect its survival and reproduction, but the degree to which colonies vary in their defensive response is poorly known. We documented considerable variation in damage to the external nest mound of Pogonomyrmex occidentalis (Cresson) at our long-term study site in 2012. Heavily damaged colonies formed several spatial clusters, consistent with predation within a home range. We tested two competing hypotheses for the variation in nest damage: (1) colonies vary in their level of aggression, those with stronger defensive responses are better protected, and suffer less damage, versus (2) colonies have similar levels of aggression, but those that suffer predator-induced damage subsequently display a stronger defense response. We measured the alarm/aggression response in colonies exposed to a standardized stimulus and determined whether the level of aggression was correlated to the level of damage. Colonies with strong defensive responses were significantly less likely to exhibit damage than those with weak responses, suggesting that aggression level is a colony phenotype.     

Non‐native mangroves support carbon storage,sediment carbon burial,and accretion of coastal ecosystems

Mangrove forests play an important role in climate change adaptation and mitigation by maintaining coastline elevations relative to sea level rise, protecting coastal infrastructure from storm damage, and storing substantial quantities of carbon (C) in live and detrital pools. Determining the efficacy of mangroves in achieving climate goals can be complicated by difficulty in quantifying C inputs (i.e., differentiating newer inputs from younger trees from older residual C pools), and mitigation assessments rarely consider potential offsets to CO₂ storage by methane (CH₄) production in mangrove sediments. The establishment of non‐native Rhizophora mangle along Hawaiian coastlines over the last century offers an opportunity to examine the role mangroves play in climate mitigation and adaptation both globally and locally as novel ecosystems. We quantified total ecosystem C storage, sedimentation, accretion, sediment organic C burial and CH₄ emissions from ~70 year old R. mangle stands and adjacent uninvaded mudflats. Ecosystem C stocks of mangrove stands exceeded mudflats by 434 ± 33 Mg C/ha, and mangrove establishment increased average coastal accretion by 460%. Sediment organic C burial increased 10‐fold (to 4.5 Mg C ha^?1 year^?1), double the global mean for old growth mangrove forests, suggesting that C accumulation from younger trees may occur faster than previously thought, with implications for mangrove restoration. Simulations indicate that increased CH₄ emissions from sediments offset ecosystem CO₂ storage by only 2%–4%, equivalent to 30–60 Mg CO₂‐eq/ha over mangrove lifetime (100 year sustained global warming potential). Results highlight the importance of mangroves as novel systems that can rapidly accumulate C, have a net positive atmospheric greenhouse gas removal effect, and support shoreline accretion rates that outpace current sea level rise. Sequestration potential of novel mangrove forests should be taken into account when considering their removal or management, especially in the context of climate mitigation goals.     

The heparan sulfate proteoglycan agrin modulates neurite outgrowth mediated by FGF-2

Although the role of agrin in the formation of the neuromuscular junction is well established, other functions for agrin have remained elusive. The present study was undertaken to assess the role of agrin in neurite outgrowth mediated by the heparin-binding growth factor basic fibroblast growth factor (FGF-2), which we have shown previously to bind to agrin with high affinity and that has been shown to mediate neurite outgrowth from a number of neuronal cell types. Using both an established neuronal cell line, PC12 cells, and primary chick retina neuronal cultures, we find that agrin potentiates the ability of FGF-2 to stimulate neurite outgrowth. In PC12 cells and retinal neurons agrin increases the efficacy of FGF-2 stimulation of neurite outgrowth mediated by the FGF receptor, as an inhibitor of the FGF receptor abolished neurite outgrowth in the presence of agrin and FGF-2. We also examined possible mechanisms by which agrin may modulate neurite outgrowth, analyzing ERK phosphorylation and c-fos phosphorylation. These studies indicate that agrin augments a transient early phosphorylation of ERK in the presence of FGF-2, and augments and sustains FGF-2 mediated increases in c-fos phosphorylation. These data are consistent with established mechanisms where heparan sulfate proteoglycans such as agrin may increase the affinity between FGF-2 and the FGF receptor. In summary, our studies suggest that neural agrin contributes to the establishment of axon pathways by modulating the function of neurite promoting molecules such as FGF-2.     

Two deep-sea spiny eels, <Emphasis Type="Italic">Notacanthus abbotti</Emphasis> and <Emphasis Type="Italic">Lipogenys gillii</Emphasis> (Albuliformes: Notacanthidae), from the Hawaiian Archipelago and Emperor Seamounts with notes on their identification and biogeography

Deep-sea spiny eels (Notacanthidae) were previously reported from the Hawaiian Archipelago; however, these reports lacked detailed information to confirm the identity of the species. We provide collection and taxonomic data for the earlier records. The first central Pacific specimen of Lipogenys gillii is reported from Hawai’i Island. A record of Notacanthus abbotti from the Hancock Seamounts, at the northern end of the Archipelago, is confirmed. Specimens from Maui, main Hawaiian Islands, previously reported as N. chemnitzii, are reidentified as N. abbotti. The Hawaiian records of notacanthids are the only reports of the family from the Pacific tectonic plate.     

Environmentally-induced shifts in behavior intensify indirect competition by an invasive gecko in Mauritius

Most recorded extinctions have occurred on oceanic islands, mainly as a result of introduced mammalian predators. The impact of introduced non-mammalian competitors, however, is poorly understood. The house gecko, Hemidactylus frenatus, is one of the most successful invasive reptiles and has been implicated in the decline of endemic geckos and other taxa on a number of tropical and subtropical islands. We investigated the patterns of niche utilization between the house gecko and endemic ornate day gecko, Phelsuma ornata, in Mauritius, two species which were not believed to compete because they had different diel activity periods. The dietary and temporal niche partitioning of the two species were examined in relation to seasonal invertebrate prey abundance for three seasons. Dietary overlap between the two species was least when prey abundance was lowest and temporal overlap in activity greatest. Exploitative competition was therefore inferred, whereby changes in dietary overlap were attributed to shifts in prey selection by the day, but not the house, gecko, which was hypothesized to deplete prey. The compensatory response of the day gecko may have been to increase its tendency for cannibalism, such that the smaller house gecko was indirectly responsible for population reduction of its larger competitor. This is the first study to show how an invasive nocturnal gecko may be affecting a predominantly diurnal species.